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01-09-2019 | Brief Report

Characterization and complete genome sequence analysis of phage GP4, a novel lytic Bcep22-like podovirus

Authors: Ronghuan Wang, Yu Cong, Zhiqiang Mi, Hang Fan, Taoxing Shi, Hui Liu, Yigang Tong

Published in: Archives of Virology | Issue 9/2019

Abstract

We isolated a novel lytic phage of Ralstonia solanacearum, GP4. The GP4 phage has a latent period of ~ 2 h at its optimal multiplicity of infection and is stable at temperatures ranging from 40–70 °C. GP4 lysed 16 strains of R. solanacearum belonging to phylotype IV. High-throughput sequencing revealed that GP4 has a linear dsDNA genome that consists of 61,129 bp, contains 83 open reading frames, and encodes a tRNA for cysteine. The GP4 genome has low similarity to other phage sequences in the GenBank database. Phylogenetic analysis indicated that GP4 can be taxonomically classified as a member of the Bcep22-like subfamily of the family Podoviridae.

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Title: Characterization and complete genome sequence analysis of phage GP4, a novel lytic Bcep22-like podovirus
Authors: Ronghuan Wang
Yu Cong
Zhiqiang Mi
Hang Fan
Taoxing Shi
Hui Liu
Yigang Tong
Publication date: 01-09-2019
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 9/2019
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-019-04309-7

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