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01-04-2019 | Annotated Sequence Record

Genetic characterization of a novel G9P[23] rotavirus A strain identified in southwestern China with evidence of a reassortment event between human and porcine strains

Authors: Danyu Chen, Long Zhou, Yiming Tian, Xuan Wu, Lan Feng, Xiping Zhang, Zhihui Liu, Shurui Pang, Runmin Kang, Jifeng Yu, Yonggang Ye, Hongning Wang, Xin Yang

Published in: Archives of Virology | Issue 4/2019

Abstract

Group A rotaviruses (RVAs) are important zoonotic pathogens that cause intestinal disease in humans and other mammals. In this study, the novel strain RVA/Pig/China/SC11/2017/G9P[23](SC11) was isolated from fecal samples from a pig farm in Sichuan province, southwestern China. The complete genome was found to be 18,347 bp in length with 11 segments. The genotype constellation of strain SC11 was G9-P[23]-I12-R1-C1-M1-A1-N1-T1-E1-H1, according to whole-genome sequencing analysis. The VP1, VP2, VP4, VP6, NSP1–NSP3, and NSP5 genes of RVA strain SC11 were found to be closely related to those of porcine and/or porcine-like human RVAs. Meanwhile, the VP7 and NSP4 genes of strain SC11 were closely related to genes of human RVAs. However, it was difficult to pinpoint the porcine or human origin of the VP3 gene of strain SC11 based on the available data. These results showed that SC11 originated from a natural reassortment event between human and pig RVA strains, and crossover points for recombination were identified at nucleotides (nt) 109-806 of NSP2. This is the first report of such a reassortant and recombinant RVA strain in the southwestern region of China.

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Title: Genetic characterization of a novel G9P[23] rotavirus A strain identified in southwestern China with evidence of a reassortment event between human and porcine strains
Authors: Danyu Chen
Long Zhou
Yiming Tian
Xuan Wu
Lan Feng
Xiping Zhang
Zhihui Liu
Shurui Pang
Runmin Kang
Jifeng Yu
Yonggang Ye
Hongning Wang
Xin Yang
Publication date: 01-04-2019
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 4/2019
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-019-04188-y

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