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01-04-2019 | Original Article

Assessment of resistance to rice tungro disease in popular rice varieties in India by introgression of a transgene against Rice tungro bacilliform virus

Authors: G. Kumar, M. Jyothsna, P. Valarmathi, S. Roy, A. Banerjee, J. Tarafdar, B. K. Senapati, S. Robin, S. Manonmani, R. Rabindran, I. Dasgupta

Published in: Archives of Virology | Issue 4/2019

Abstract

Rice crops in South and Southeast Asian countries suffer critical yield losses due to rice tungro disease caused by joint infection with rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV). Previously, for generating RNA interference-based transgenic resistance against tungro viruses, RTBV ORF IV was used as a transgene to develop RTBV resistance in a popular high-yielding scented rice variety. The transgene from this line was then introgressed into five popular high-yielding but tungro-susceptible rice varieties by marker-assisted backcross breeding with a view to combine the resistant trait with the agronomic traits. The present work includes a resistance assay of the BC₃F₅ lines of these varieties under glasshouse conditions. Out of a total of 28 lines tested, each consisting of 12 individual plants, eight lines showed significant amelioration in height reduction and 100- to 1000-fold reduction in RTBV titers. The RNAi-mediated resistance was clearly manifested by the presence of virus-derived small RNA (vsRNA) specific for RTBV ORF IV in the transgenic backcrossed lines.

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Title: Assessment of resistance to rice tungro disease in popular rice varieties in India by introgression of a transgene against Rice tungro bacilliform virus
Authors: G. Kumar
M. Jyothsna
P. Valarmathi
S. Roy
A. Banerjee
J. Tarafdar
B. K. Senapati
S. Robin
S. Manonmani
R. Rabindran
I. Dasgupta
Publication date: 01-04-2019
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 4/2019
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-019-04159-3

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