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Archives of Virology
Published in: Archives of Virology 11/2018

01-11-2018 | Original Article

Cor interacts with outer membrane proteins to exclude FhuA-dependent phages

Authors: Emma S. Arguijo-Hernández, Javier Hernandez-Sanchez, Saida J. Briones-Peña, Norma Oviedo, Guillermo Mendoza-Hernández, Gabriel Guarneros, Luis Kameyama

Published in: Archives of Virology | Issue 11/2018

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Abstract

Superinfection exclusion (Sie) of FhuA-dependent phages is carried out by Cor in the Escherichia coli mEp167 prophage lysogenic strain. In this work, we present evidence that Cor is an outer membrane (OM) lipoprotein that requires the participation of additional outer membrane proteins (OMPs) to exclude FhuA-dependent phages. Two Cor species of ~13 and ~8.5 kDa, corresponding to the preprolipoprotein/prolipoprotein and lipoprotein, were observed by Western blot. Cell mutants for CorC17F, CorA18D and CorA57E lost the Sie phenotype for FhuA-dependent phages. A copurification affinity binding assay combined with LC_ESI_MS/MS showed that Cor bound to OMPs: OmpA, OmpC, OmpF, OmpW, LamB, and Slp. Interestingly, Sie for FhuA-dependent phages was reduced on Cor overexpressing FhuA+ mutant strains, where ompA, ompC, ompF, ompW, lamB, fhuE, genes were knocked out. The exclusion was restored when these strains were supplemented with plasmids expressing these genes. Sie was not lost in other Cor overexpressing FhuA+ null mutant strains JW3938(btuB-), JW5100(tolB-), JW3474(slp-). These results indicate that Cor interacts and requires some OMPs to exclude FhuA-dependent phages.
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Metadata
Title
Cor interacts with outer membrane proteins to exclude FhuA-dependent phages
Authors
Emma S. Arguijo-Hernández
Javier Hernandez-Sanchez
Saida J. Briones-Peña
Norma Oviedo
Guillermo Mendoza-Hernández
Gabriel Guarneros
Luis Kameyama
Publication date
01-11-2018
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 11/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-018-3954-z

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