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01-09-2018 | Brief Report

Phylogeographic analysis of porcine reproductive and respiratory syndrome virus 1 in Guangdong province, Southern China

Authors: Shao-Lun Zhai, Tao Lin, Xia Zhou, Zhang-Fu Pei, Zu-Zhang Wei, He Zhang, Xiao-Hui Wen, Qin-Ling Chen, Dian-Hong Lv, Wen-Kang Wei

Published in: Archives of Virology | Issue 9/2018

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is considered an important economic pathogen for the international swine industry. At present, both PRRSV-1 and PRRSV-2 have been confirmed to be co-circulating in China. However, there is little available information about the prevalence or distribution of PRRSV-1 in Guangdong province, southern China. In this study, we performed molecular detection of PRRSV-1 in 750 samples collected from 50 farms in 15 major pig farming regions in this province. After RT-PCR testing, 64% (32/50) of farms were confirmed as PRRSV-1-positive. Surprisingly, PRRSV-1 was circulating on at least one pig farm in all 15 regions; of the 750 samples, 186 samples (24.8%) were positive for PRRSV-1. Furthermore, 15 representative PRRSV-1 ORF5 sequences (606 bp) (n = 1 per region) were obtained from those PRRSV-1-positive regions. Sequence alignment analysis indicated that they shared 81.8% ~ 100% nucleotide and 81.2% ~ 100% amino acid similarity with each other. Although all current PRRSV-1 sequences were divided into pandemic subtype 1, most of them had unique glycoprotein-5 amino acid sequences that are significantly different from other known PRRSV-1 isolates. To conclude, the present findings revealed wide geographical distribution of PRRSV-1 in Guangdong province, southern China. This study further extends the epidemiological significance of PRRSV-1 in China.

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Title: Phylogeographic analysis of porcine reproductive and respiratory syndrome virus 1 in Guangdong province, Southern China
Authors: Shao-Lun Zhai
Tao Lin
Xia Zhou
Zhang-Fu Pei
Zu-Zhang Wei
He Zhang
Xiao-Hui Wen
Qin-Ling Chen
Dian-Hong Lv
Wen-Kang Wei
Publication date: 01-09-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 9/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-3873-z

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