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01-09-2018 | Original Article

Analysis of genetic variability of respiratory syncytial virus groups A and B in Kuwait

Authors: Nada Madi, Wassim Chehadeh, Mohammed Asadzadeh, Mariam Al-Turab, Anfal Al-Adwani

Published in: Archives of Virology | Issue 9/2018

Abstract

Respiratory syncytial virus (RSV) is the most frequently identified viral agent in infants, children, and elderly people with acute respiratory tract infections (ARTIs). This study is the only one of its kind in Kuwait, and its purpose was to investigate the genetic variability of the G protein gene in RSV strains prevalent in Kuwait. Respiratory samples were collected from patients with ARTIs in various hospitals in Kuwait and subjected to reverse transcription PCR (RT-PCR) amplifying a fragment of the G gene of RSV. A total of 305 samples were collected between January and mid-December 2016, and 77 (25.2%) were positive for RSV. Group A viruses were predominant over group B viruses; the RSV-A group was detected in 52 (67.5%) of the positive samples, while the RSV-B group was detected in 25 (32.5%) of the positive samples. Phylogenetic analysis showed that all RSV-A strains grouped into eight clusters of identical sequences of untyped strains. Twelve RSV-B strains, on the other hand, belonged to the RSV-B/BA10 genotype, while the rest were untyped. These data suggest that new and untyped strains of RSV-A group likely predominated in Kuwait and that the BA10 genotype of the RSV-B group became the dominant genotype in the 2016 season.

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Title: Analysis of genetic variability of respiratory syncytial virus groups A and B in Kuwait
Authors: Nada Madi
Wassim Chehadeh
Mohammed Asadzadeh
Mariam Al-Turab
Anfal Al-Adwani
Publication date: 01-09-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 9/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-3881-z

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