Skip to main content
Top
Published in: Archives of Virology 9/2018

01-09-2018 | Rapid Communication

Retrospective survey and phylogenetic analysis of porcine circovirus type 3 in Jiangsu province, China, 2008 to 2017

Authors: Dong Zhao, Xiaobo Wang, Qingqing Gao, Changchao Huan, Wanbin Wang, Song Gao, Xiufan Liu

Published in: Archives of Virology | Issue 9/2018

Login to get access

Abstract

Porcine circovirus type 3 (PCV3) is a novel porcine circovirus recently identified in pigs associated with porcine dermatitis nephropathy syndrome, reproductive failure and multi-systemic inflammation. This study aimed to identify PCV3 in clinical samples from pigs collected between 2008 and 2017 in Jiangsu province, China. A total of 272 pig tissue samples from 141 pig farms in Jiangsu province were examined and analyzed. Forty of the 272 (14.7%) samples tested were positive for PCV3, while 28/40 (70%) of the PCV3-positive samples were co-infected with PCV2. Among them, 18, 1, 14 and 7 of the PCV3-positive samples were identified in 2013, 2014, 2015 and 2017, respectively. The complete genome sequences from four of the PCV3s contained 2,000 nucleotides, and shared 98.6% to 99.6% nucleotide sequence identity with the PCV3 isolates available in GenBank. Our results indicate that a large outbreak of PCV3 occurred in Jiangsu province pig herds in 2013, after which a fairly stable infection rate was recorded. It is imperative, therefore, to gain a better understanding of the pathogenicity of PCV3 and control its further dissemination in this region.
Literature
1.
go back to reference Allan GM, McNeilly F, Kennedy S, Daft B, Ellis JA, Haines DM, Adair BM (1998) Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. J Vet Diagn Investig 10(1):3–10CrossRef Allan GM, McNeilly F, Kennedy S, Daft B, Ellis JA, Haines DM, Adair BM (1998) Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe. J Vet Diagn Investig 10(1):3–10CrossRef
2.
go back to reference Faccini S, Barbieri I, Gilioil A, Sala G, Gibelli LR, Moreno A, Nigrelli A (2017) Detection and genetic characterization of Porcine circovirus type 3 in Italy. Transbound Emerg Dis 64(6):1661–1664CrossRefPubMed Faccini S, Barbieri I, Gilioil A, Sala G, Gibelli LR, Moreno A, Nigrelli A (2017) Detection and genetic characterization of Porcine circovirus type 3 in Italy. Transbound Emerg Dis 64(6):1661–1664CrossRefPubMed
3.
go back to reference Fan S, Ku X, Chen F, Wang Y, Yu X, He Q (2017) Complete genome sequence of a novel porcine circovirus type 3 strain, PCV3/CN/hubei-618/2016, isolated from China. Genome Announc 5(15):1–2CrossRef Fan S, Ku X, Chen F, Wang Y, Yu X, He Q (2017) Complete genome sequence of a novel porcine circovirus type 3 strain, PCV3/CN/hubei-618/2016, isolated from China. Genome Announc 5(15):1–2CrossRef
4.
go back to reference Gao X, Dong X, Wang X, Gao S (2014) Diagnosis of a mixed infection of porcine Pasteurella pasteurella and porcine circovirus type 2. Heilongjiang Anim Sci Vet Med 2:124–126 (in Chinese) Gao X, Dong X, Wang X, Gao S (2014) Diagnosis of a mixed infection of porcine Pasteurella pasteurella and porcine circovirus type 2. Heilongjiang Anim Sci Vet Med 2:124–126 (in Chinese)
5.
go back to reference Ku X, Chen F, Li P, Wang Y, Yu X, Fan S, He Q (2017) Identification and genetic characterization of porcine circovirus type 3 in China. Transbound Emerg Dis 64(3):703–708CrossRefPubMed Ku X, Chen F, Li P, Wang Y, Yu X, Fan S, He Q (2017) Identification and genetic characterization of porcine circovirus type 3 in China. Transbound Emerg Dis 64(3):703–708CrossRefPubMed
6.
go back to reference Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874CrossRefPubMed Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874CrossRefPubMed
7.
go back to reference Mankertz A, Caliskan R, Hattermann K, Hillenbrand BP, Mueller B, Finsterbusch T (2004) Molecular biology of porcine circovirus: analyses of gene expression and viral replication. Vet Microbiol 98(2):81–88CrossRefPubMed Mankertz A, Caliskan R, Hattermann K, Hillenbrand BP, Mueller B, Finsterbusch T (2004) Molecular biology of porcine circovirus: analyses of gene expression and viral replication. Vet Microbiol 98(2):81–88CrossRefPubMed
8.
go back to reference Opriessnig T, Meng XJ, Halbur PG (2007) Porcine circovirus type 2-associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J Vet Diagn Investig 19(6):591–615CrossRef Opriessnig T, Meng XJ, Halbur PG (2007) Porcine circovirus type 2-associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J Vet Diagn Investig 19(6):591–615CrossRef
9.
go back to reference Palinski R, Pineyro P, Shang P, Yuan F, Guo R, Fang Y, Hause BM (2017) A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure. J Virol 91:e01879-16CrossRefPubMed Palinski R, Pineyro P, Shang P, Yuan F, Guo R, Fang Y, Hause BM (2017) A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure. J Virol 91:e01879-16CrossRefPubMed
10.
go back to reference Phan TG, Giannitti F, Rossow S, Marthaler D, Knutson T, Li L, Delwart E (2016) Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation. Virol J 13(1):184CrossRefPubMedPubMedCentral Phan TG, Giannitti F, Rossow S, Marthaler D, Knutson T, Li L, Delwart E (2016) Detection of a novel circovirus PCV3 in pigs with cardiac and multi-systemic inflammation. Virol J 13(1):184CrossRefPubMedPubMedCentral
11.
go back to reference Shen H, Liu X, Zhang P, Wang L, Liu Y, Zhang L, Song C (2017) Genome characterization of a porcine circovirus type 3 in South China. Transbound Emerg Dis 65(1):264–266CrossRefPubMed Shen H, Liu X, Zhang P, Wang L, Liu Y, Zhang L, Song C (2017) Genome characterization of a porcine circovirus type 3 in South China. Transbound Emerg Dis 65(1):264–266CrossRefPubMed
12.
go back to reference Stadejek T, Wozniak A, Milek D, Biernacka K (2017) First detection of porcine circovirus type 3 on commercial pig farms in Poland. Transbound Emerg Dis 64(5):1350–1353CrossRefPubMed Stadejek T, Wozniak A, Milek D, Biernacka K (2017) First detection of porcine circovirus type 3 on commercial pig farms in Poland. Transbound Emerg Dis 64(5):1350–1353CrossRefPubMed
13.
go back to reference Tischer I, Mields W, Wolff D, Vagt M, Griem W (1986) Studies on epidemiology and pathogenicity of porcine circovirus. Arch Virol 91(3–4):271–276CrossRefPubMed Tischer I, Mields W, Wolff D, Vagt M, Griem W (1986) Studies on epidemiology and pathogenicity of porcine circovirus. Arch Virol 91(3–4):271–276CrossRefPubMed
14.
go back to reference Tischer I, Rasch R, Tochtermann G (1974) Characterization of papovavirus and picornavirus-like particles in permanent pig kidney cell lines. Zent Bakteriol 226(2):153–167 Tischer I, Rasch R, Tochtermann G (1974) Characterization of papovavirus and picornavirus-like particles in permanent pig kidney cell lines. Zent Bakteriol 226(2):153–167
15.
go back to reference Tochetto C, Lima DA, Varela APM, Loiko MR, Paim WP, Roehe PM (2017) Full-genome sequence of porcine circovirus type 3 recovered from serum of sows with stillbirths in Brazil. Transbound Emerg Dis 65(1):5–9CrossRefPubMed Tochetto C, Lima DA, Varela APM, Loiko MR, Paim WP, Roehe PM (2017) Full-genome sequence of porcine circovirus type 3 recovered from serum of sows with stillbirths in Brazil. Transbound Emerg Dis 65(1):5–9CrossRefPubMed
16.
go back to reference Zheng S, Wu X, Zhang L, Xin C, Liu Y, Shi J, Wang J (2017) The occurrence of porcine circovirus 3 without clinical infection signs in Shandong Province. Transbound Emerg Dis 64(5):1337–1341CrossRefPubMed Zheng S, Wu X, Zhang L, Xin C, Liu Y, Shi J, Wang J (2017) The occurrence of porcine circovirus 3 without clinical infection signs in Shandong Province. Transbound Emerg Dis 64(5):1337–1341CrossRefPubMed
Metadata
Title
Retrospective survey and phylogenetic analysis of porcine circovirus type 3 in Jiangsu province, China, 2008 to 2017
Authors
Dong Zhao
Xiaobo Wang
Qingqing Gao
Changchao Huan
Wanbin Wang
Song Gao
Xiufan Liu
Publication date
01-09-2018
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 9/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-018-3870-2

Other articles of this Issue 9/2018

Archives of Virology 9/2018 Go to the issue