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01-06-2018 | Annotated Sequence Record

Genomic characteristics of vB_PpaP_PP74, a T7-like Autographivirinae bacteriophage infecting a potato pathogen of the newly proposed species Pectobacterium parmentieri

Authors: Anastasia Kabanova, Mikhail Shneider, Eugenia Bugaeva, Vo Thi Ngoc Ha, Kirill Miroshnikov, Aleksei Korzhenkov, Eugene Kulikov, Stepan Toschakov, Alexander Ignatov, Konstantin Miroshnikov

Published in: Archives of Virology | Issue 6/2018

Abstract

Bacteriophage vB_PpaP_PP74 (PP74) is a novel virulent phage that infects members of the species Pectobacterium parmentieri, a newly established species of soft-rot-causing bacteria in the family Pectobacteriaceae, derived from potato-specific Pectobacterium wasabiae. vB_PpaP_PP74 was identified as a member of the family Podoviridae by transmission electron microscopy. The phage has a 39,790-bp dsDNA genome containing 50 open reading frames (ORFs). Because of the absence of genes encoding toxins or lysogeny factors, PP74 may be considered a candidate phage for pathogen biocontrol applications. The genome layout is similar to genomes of T7-like phages within the subfamily Autographivirinae, and therefore, functions can be attributed to most of ORFs. However, the closest nucleotide sequence homologs of phage PP74 are unclassified Escherichia phages. Based on phylogenetic analysis, vB_PpaP_PP74 is a sensu lato T7-like phage, but it forms a distant subgenus group together with homologous enterobacterial phages.

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Title: Genomic characteristics of vB_PpaP_PP74, a T7-like Autographivirinae bacteriophage infecting a potato pathogen of the newly proposed species Pectobacterium parmentieri
Authors: Anastasia Kabanova
Mikhail Shneider
Eugenia Bugaeva
Vo Thi Ngoc Ha
Kirill Miroshnikov
Aleksei Korzhenkov
Eugene Kulikov
Stepan Toschakov
Alexander Ignatov
Konstantin Miroshnikov
Publication date: 01-06-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 6/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-018-3766-1

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Abstract

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