Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 6/2018

01-06-2018 | Original Article

Development of a reverse transcription recombinase-aided amplification assay for the detection of coxsackievirus A10 and coxsackievirus A6 RNA

Authors: Teng-fei Yan, Xin-na Li, Le Wang, Chen Chen, Su-xia Duan, Ju-ju Qi, Li-Xin Li, Xue-jun Ma

Published in: Archives of Virology | Issue 6/2018

Login to get access

Abstract

Hand, foot and mouth disease (HFMD) is a serious public health problem, and coxsackievirus A6 (CVA6) and coxsackievirus A10 (CVA10) are two of the major causative pathogens, in addition to enterovirus 71 (EV71) and coxsackievirus A16 (CVA16). A simple and rapid reverse transcription recombinase-aided amplification assay (RT-RAA) was developed for the detection of CVA10 and CVA6 in this study. The analytical sensitivity for detection of CVA10 and CVA6 at 95% probability by probit regression analysis was 35 copies per reaction and 38 copies per reaction, respectively, with 100% specificity. Compared with commercial RT-qPCR assays, when testing 455 fecal specimens, the kappa value of the RT-RAA assay for CVA10 and CVA6 was 0.920 (p < 0.001) and 0.952 (p < 0.001), respectively. Moreover, four samples that were positive for CVA10 and five that were positive for CVA6 by RT-RAA but negative by RT-qPCR were further determined to be true positives. These results demonstrate that the proposed RT-RAA assays are very valuable tools for the detection of CVA10 and CVA6 and have potential for use in resource-limited settings.
Literature
1.
Solomon T, Lewthwaite P, Perera D, Cardosa MJ, Mcminn P, Ooi MH (2010) Virology, epidemiology, pathogenesis, and control of enterovirus 71. Lancet Infectious Dis 10(11):778–790CrossRef
2.
Yip CCY, Lau SKP, Woo PCY, Yuen KY (2013) Human enterovirus 71 epidemics: what’s next? Emerging Health Threats J 6(6):19780CrossRef
3.
Lo SH, Huang YC, Huang CG, Tsao KC, Li WC, Hsieh YC, Chiu CH, Lin TY (2011) Clinical and epidemiologic features of Coxsackievirus A6 infection in children in northern Taiwan between 2004 and 2009. J Microbiol, Immunol, Infect 44(4):252CrossRef
4.
Tryfonos C, Richter J, Koptides D, Yiangou M, Christodoulou CG (2011) Molecular typing and epidemiology of enteroviruses in Cyprus, 2003–2007. J Med Microbiol 60(10):1433–1440CrossRefPubMed
5.
Gopalkrishna V, Patil PR, Patil GP, Chitambar SD (2012) Circulation of multiple enterovirus serotypes causing hand, foot and mouth disease in India. J Med Microbiol 61(Pt 3):420CrossRefPubMed
6.
Yang F, Ren L, Xiong Z, Li J, Xiao Y, Zhao R, He Y, Bu G, Zhou S, Wang J (2009) Enterovirus 71 Outbreak in the People’s Republic of China in 2008. J Clin Microbiol 47(7):2351CrossRefPubMedPubMedCentral
7.
Oberste MS, Maher K, Kilpatrick DR, Pallansch MA (1999) Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification. J Virol 73(3):1941–1948PubMedPubMedCentral
8.
Perera D, Shimizu H, Yoshida H, Tu PV, Ishiko H, Mcminn PC, Cardosa MJ (2010) A comparison of the VP1, VP2, and VP4 regions for molecular typing of human enteroviruses. J Med Virol 82(4):649–657CrossRefPubMed
9.
Zhu RN, Qian Y, Deng J, Xing JF, Zhao LQ, Wang F, Liao B, Ren XX, Li Y (2007) Zhang Q (2007) [Study on the association of hand, foot and mouth disease and enterovirus 71/CA16 among children in Beijing. Chin J Epidemiol 28(10):1004–1008
10.
Bible JM, Pantelidis P, Chan PK, Tong CY (2007) Genetic evolution of enterovirus 71: epidemiological and pathological implications. Rev Med Virol 17(6):371–379CrossRefPubMed
11.
Yan W, Yeo A, Phoon MC, Tan EL, Poh CL, Quak SH, Chow VTK (2010) The largest outbreak of hand; foot and mouth disease in Singapore in 2008: the role of enterovirus 71 and coxsackievirus A strains. Int J Infect Dis 14(12):e1076CrossRef
12.
Mirand A, Henquell C, Archimbaud C, Ughetto S, Antona D, Bailly JL, Peigue-Lafeuille H (2012) Outbreak of hand, foot and mouth disease/herpangina associated with coxsackievirus A6 and A10 infections in 2010, France: a large citywide, prospective observational study. Clin Microbiol Infect 18(5):E110–E118CrossRefPubMed
13.
Blomqvist S, Klemola P, Kaijalainen S, Paananen A, Simonen ML, Vuorinen T, Roivainen M (2010) Co-circulation of coxsackieviruses A6 and A10 in hand, foot and mouth disease outbreak in Finland. J Clin Virol 48(1):49–54CrossRefPubMed
14.
Javier LDMD, Pablo HBMD, Violeta ZNMD, María ABPD, Fernando G-CPD, Antonio SMD, Gobernado M, Isabel FBMD (2011) Onychomadesis outbreak in valencia, spain associated with hand, foot, and mouth disease caused by enteroviruses. Pediatr Dermatol 28(1):1–5CrossRef
15.
Xu M, Su L, Cao L, Zhong H, Dong N, Xu J (2013) Enterovirus genotypes causing hand foot and mouth disease in Shanghai, China: a molecular epidemiological analysis. BMC Infect Dis 13(1):489CrossRefPubMedPubMedCentral
16.
Chonmaitree T, Ford C, Sanders C, Lucia HL (1988) Comparison of cell cultures for rapid isolation of enteroviruses. J Clin Microbiol 26(12):2576PubMedPubMedCentral
17.
Lim KA, Benyeshmelnick M (1960) Typing of viruses by combinations of antiserum pools. Application to typing of enteroviruses (Coxsackie and ECHO). J Immunol 84(3):309–317PubMed
18.
Yolken RH, Torsch VM (1981) Enzyme-linked immunosorbent assay for detection and identification of coxsackieviruses A. Infect Immun 31(2):742–750PubMedPubMedCentral
19.
Hu XM, Zhang Y, Xu BL, Yang MJ, Wang M, Zhang C, Li J, Bai RY, Zhou XM, Xu WB (2011) Development of a GeXP based multiplex RT-PCR assay for simultaneous differentiation of nine human hand food mouth disease pathogens. Bing Du Xue Bao 27(27):331–336PubMed
20.
Fuschino ME, Lamson DM, Rush K, Carbone LS, Taff ML, Hua Z, Landi K, George KS (2012) Detection of coxsackievirus A10 in multiple tissues of a fatal infant sepsis case. J Clin Virol Off Publ Pan Am Soc Clin Virol 53(3):259–261CrossRef
21.
Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:E63. Nucleic Acids Res 28(12):E63CrossRefPubMedPubMedCentral
22.
Nagamine K, Hase T, Notomi T (2002) Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16(3):223CrossRefPubMed
23.
Zhang X, Guo L, Ma R, Cong L, Wu Z, Wei Y, Xue S, Zheng W, Tang S (2017) Rapid detection of Salmonella with recombinase aided amplification. J Microbiol Methods 139:202–204CrossRefPubMed
24.
Bei L, Cheng HR, Yan QF, Huang ZJ, Shen GF, Zhang ZF, Yinv LI, Deng ZX, Lin M (2010) Recombinase-Aid Amplification:a Novel Technology of in vitro Rapid Nucleic Acid Amplification. Scientia Sinica
25.
26.
Nie K, Zhang Y, Luo L, Yang MJ, Hu XM, Wang M, Zhu SL, Han F, Xu WB, Ma XJ (2011) Visual detection of human enterovirus 71 subgenotype C4 and Coxsackievirus A16 by reverse transcription loop-mediated isothermal amplification with the hydroxynaphthol blue dye. J Virol Methods 175(2):283–286CrossRefPubMed
27.
Nie K, Qi SX, Zhang Y, Luo L, Xie Y, Yang MJ, Zhang Y, Li J, Shen H, Li Q (2012) Evaluation of a direct reverse transcription loop-mediated isothermal amplification method without RNA extraction for the detection of human enterovirus 71 subgenotype C4 in nasopharyngeal swab specimens. PLoS One 7(12):e52486CrossRefPubMedPubMedCentral
28.
Guan H, Wang J, Wang C, Yang M, Liu L, Yang G, Ma X (2015) Etiology of multiple non-EV71 and non-CVA16 enteroviruses Associated with Hand, Foot and Mouth Disease in Jinan, China, 2009-June 2013. PLoS One 10(11):e0142733CrossRefPubMedPubMedCentral
29.
Tian H, Zhang Y, Sun Q, Zhu S, Li X, Pan Z, Xu W, Xu B (2014) Prevalence of Multiple Enteroviruses Associated with Hand, Foot, and Mouth Disease in Shijiazhuang City, Hebei Province, China: Outbreaks of Coxsackieviruses A10 and B3. PLoS One 9(1):e84233CrossRefPubMedPubMedCentral
30.
Oberste M, Maher K, Williams A, Dybdahl-Sissoko N, Brown B, Gookin M, Penaranda S, Mishrik N, Uddin M, Pallansch M (2006) Species-specific RT-PCR amplification of human enteroviruses: a tool for rapid species identification of uncharacterized enteroviruses. J Gen Virol 87(Pt 1):119CrossRefPubMed
31.
Nasri D, Bouslama L, Pillet S, Bourlet T, Aouni M, Pozzetto B (2007) Basic rationale, current methods and future directions for molecular typing of human enterovirus. Expert Rev Mol Diagn 7(4):419–434CrossRefPubMed
32.
Wang K, Wu Y, Yin D, Tang S, Hu G, He Y (2016) Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6. Archives of Virology 1–4
33.
Malek L, Sooknanan R, Compton J (2008) Nucleic acid sequence-based amplification (NASBA™). Humana Press
34.
Brentano ST, Mcdonough SH (2000) Isothermal amplification of RNA by transcription-mediated amplification (TMA). Springer, BerlinCrossRef
35.
Lizardi PM, Huang X, Zhu Z, Brayward P, Thomas DC, Ward DC (1998) Mutation detection and single-molecule counting using isothermal rolling-circle amplification. Nat Genet 19(3):225–232CrossRefPubMed
36.
Ding X, Nie K, Shi L, Zhang Y, Guan L, Zhang D, Qi S, Ma X (2014) Improved detection limit in rapid detection of human enterovirus 71 and coxsackievirus A16 by a novel reverse transcription-isothermal multiple-self-matching-initiated amplification assay. J Clin Microbiol 52(6):1862–1870CrossRefPubMedPubMedCentral
37.
Wang J, Li R, Hu L, Sun X, Wang J, Li J (2015) Development of a quantitative fluorescence single primer isothermal amplification-based method for the detection of Salmonella. Int J Food Microbiol 219:22CrossRefPubMed
38.
Xie XP, Huang LZ, Liu RW, Sheng-Fu HE, Chen HY, Chen JC, Tang L, Hospital GP (2014) Establishment of multiple fluorescent RT-PCR for simultaneously detecting Coxsackievirus A6,10 and 16 and its clinical application. Chin J Clin Lab Sci 10:721–725
Metadata
Title
Development of a reverse transcription recombinase-aided amplification assay for the detection of coxsackievirus A10 and coxsackievirus A6 RNA
Authors
Teng-fei Yan
Xin-na Li
Le Wang
Chen Chen
Su-xia Duan
Ju-ju Qi
Li-Xin Li
Xue-jun Ma
Publication date
01-06-2018
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 6/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-018-3734-9

Other articles of this Issue 6/2018

Archives of Virology 6/2018 Go to the issue

Original Article

Involvement of CD8+ T cells in the development of renal hemorrhage in a mouse model of hemorrhagic fever with renal syndrome

Brief Report

The complete genome sequence of CrRV-Ch01, a new member of the family Rhabdoviridae in the parasitic copepod Caligus rogercresseyi present on farmed Atlantic salmon (Salmo salar) in Chile

Original Article

Development of an antigen-capture ELISA for the quantitation of equine arteritis virus in culture supernatant

Original Article

Detection of HBV genome in the plasma and peripheral blood mononuclear cells of Iranian HBsAg negative patients with HIV infection: occult HBV infection

Original Article

Host-associated selection of a P3 mutant of zucchini yellow mosaic virus affects viral infectivity in watermelon

Original Article

Characterization of the interactome of the porcine reproductive and respiratory syndrome virus glycoprotein-5
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits