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Archives of Virology
Published in: Archives of Virology 3/2018

01-03-2018 | Original Article

Equine herpesvirus type 1 ORF51 encoding UL11 as an essential gene for replication in cultured cells

Authors: Yassien Badr, Ayaka Okada, Rania Abo-Sakaya, Emad Beshir, Kenji Ohya, Hideto Fukushi

Published in: Archives of Virology | Issue 3/2018

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Abstract

Equine herpesvirus type 1 (EHV-1) UL11 is a 74-amino-acid tegument protein encoded by ORF51 of the EHV-1 genome. EHV-1 UL11 was previously reported by other researchers using the RacL22 and RacH strains to be nonessential for viral replication in cultured cells. Here, we constructed UL11 mutant viruses including a UL11 null mutant and three C-terminal truncated mutants, for further characterization of EHV-1 UL11 using bacterial artificial chromosome (BAC) technology based on the neuropathogenic strain Ab4p. EHV-1 Ab4p UL11 was localized to juxtanuclear and Golgi regions as reported by other researchers. We found that no progeny viruses were produced by transfection of fetal equine kidney cells and rabbit kidney (RK-13) cells with the UL11 null mutant and truncation mutant BAC DNAs. However, mutant viruses were generated after transfection of RK13-UL11 cells constitutively expressing EHV-1 UL11 with the mutant BAC DNAs. In conclusion, UL11 of EHV-1 Ab4p is essential for replication in cultured cells.
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Metadata
Title
Equine herpesvirus type 1 ORF51 encoding UL11 as an essential gene for replication in cultured cells
Authors
Yassien Badr
Ayaka Okada
Rania Abo-Sakaya
Emad Beshir
Kenji Ohya
Hideto Fukushi
Publication date
01-03-2018
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 3/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-017-3650-4

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