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Published in:

01-12-2017 | Original Article

miR-146a Inhibits dengue-virus-induced autophagy by targeting TRAF6

Authors: Jieying Pu, Siyu Wu, Heping Xie, Yuye Li, Zhicong Yang, Xinwei Wu, Xi Huang

Published in: Archives of Virology | Issue 12/2017

Abstract

During dengue virus (DENV) infection, the virus manipulates different cellular pathways to assure productive replication, including autophagy. However, it remains unclear how this autophagic process is regulated. Here, we have demonstrated a novel role for the microRNA miR-146a in negatively regulating the cellular autophagic pathway in DENV-infected A549 cells and THP-1 cells. Overexpression of miR-146a significantly blocked DENV2-induced autophagy, and LNA-mediated inhibition of miR-146a counteracted these effects. Moreover, co-overexpression of TRAF6, a target of miR-146a, significantly reversed the inhibitory effect of miR-146a on autophagy. Notably, treatment with recombinant IFN-β fully restored the autophagic activity in TRAF6-silenced cells. Furthermore, our data showed that, in DENV2-infected A549 cells, autophagy promoted a pro-inflammatory response to significantly increase TNF-α and IL-6 production. Taken together, our results define a novel role for miR-146a as a negative regulator of DENV-induced autophagy and identify TRAF6 as a key target of this microRNA in modulating the DENV-autophagy interaction.

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Title: miR-146a Inhibits dengue-virus-induced autophagy by targeting TRAF6
Authors: Jieying Pu
Siyu Wu
Heping Xie
Yuye Li
Zhicong Yang
Xinwei Wu
Xi Huang
Publication date: 01-12-2017
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 12/2017
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3516-9

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miR-146a Inhibits dengue-virus-induced autophagy by targeting TRAF6

Abstract

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