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01-10-2017 | Brief Report

A red squirrel associated adenovirus identified by a combined microarray and deep sequencing approach

Authors: Björn Abendroth, Dirk Höper, Rainer G. Ulrich, Gudrun Larres, Martin Beer

Published in: Archives of Virology | Issue 10/2017

Abstract

In 2013, an adult red squirrel (Sciurus vulgaris) was found dead showing, during necropsy, acute diffuse catarrhal enteritis. Using a virus screening pipeline based on a pan-viral DNA microarray with downstream next-generation sequencing a novel squirrel-associated virus was identified with mastadenovirus-typical sequence elements. Phylogenetic analysis of hexon protein amino acid sequences demonstrated the highest similarity to Equine adenovirus (AdV) 2, but a strong divergence to a squirrel AdV from Korea and other rodent AdVs. Shorter hexon gene segment investigations confirmed a close relationship with other squirrel-derived AdVs from Europe. The novel virus species was tentatively designated as Squirrel Adenovirus-1.

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Title: A red squirrel associated adenovirus identified by a combined microarray and deep sequencing approach
Authors: Björn Abendroth
Dirk Höper
Rainer G. Ulrich
Gudrun Larres
Martin Beer
Publication date: 01-10-2017
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 10/2017
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3463-5

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A red squirrel associated adenovirus identified by a combined microarray and deep sequencing approach

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