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01-09-2017 | Brief Report

Characterization of a new isolate of pepper chlorotic spot virus from Yunnan province, China

Authors: Kuanyu Zheng, Tsung-Chi Chen, Shyi-Dong Yeh, Md. Siddiqur Rahman, Xiaoxia Su, Kuo Wu, Ting Li, Zhongkai Zhang, Jiahong Dong

Published in: Archives of Virology | Issue 9/2017

Abstract

A new isolate, 14YV733, of pepper chlorotic spot virus (PCSV) from chili peppers in Yunnan province of China was identified. The typical tospoviral particles of 80–120 nm in diameter were observed by electron microscopy. The virus caused systemic symptoms in several solanaceous plants and the Brassica rapa L. Chinensis group with mechanical inoculation. The sap from infected leaves reacted positively to a rabbit antibody to the N protein of watermelon silver mottle virus (WSMoV) in immunoblotting. The S, M, and L RNAs of PCSV-14YV733 are 3310 nts, 4711 nts, and 8913 nts long, respectively. This is the first report of complete sequences of PCSV in mainland China. Phylogenetic analysis of all tospoviral proteins indicated that PCSV-14YV733 is closely related to members of the WSMoV serogroup.

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Title: Characterization of a new isolate of pepper chlorotic spot virus from Yunnan province, China
Authors: Kuanyu Zheng
Tsung-Chi Chen
Shyi-Dong Yeh
Md. Siddiqur Rahman
Xiaoxia Su
Kuo Wu
Ting Li
Zhongkai Zhang
Jiahong Dong
Publication date: 01-09-2017
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 9/2017
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3402-5

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