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01-06-2016 | Brief Report

Detection of West Nile virus and tick-borne encephalitis virus in birds in Slovakia, using a universal primer set

Authors: Tomáš Csank, Katarína Bhide, Elena Bencúrová, Saskia Dolinská, Petra Drzewnioková, Peter Major, Ľuboš Korytár, Eva Bocková, Mangesh Bhide, Juraj Pistl

Published in: Archives of Virology | Issue 6/2016

Abstract

West Nile virus (WNV) is a mosquito-borne neurotropic pathogen that presents a major public health concern. Information on WNV prevalence and circulation in Slovakia is insufficient. Oral and cloacal swabs and bird brain samples were tested for flavivirus RNA by RT-PCR using newly designed generic primers. The species designation was confirmed by sequencing. WNV was detected in swab and brain samples, whereas one brain sample was positive for tick-borne encephalitis virus (TBEV). The WNV sequences clustered with lineages 1 and 2. These results confirm the circulation of WNV in birds in Slovakia and emphasize the risk of infection of humans and horses.

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Title: Detection of West Nile virus and tick-borne encephalitis virus in birds in Slovakia, using a universal primer set
Authors: Tomáš Csank
Katarína Bhide
Elena Bencúrová
Saskia Dolinská
Petra Drzewnioková
Peter Major
Ľuboš Korytár
Eva Bocková
Mangesh Bhide
Juraj Pistl
Publication date: 01-06-2016
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 6/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-016-2828-5

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