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01-04-2016 | Brief Report

Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification

Authors: Jianchang Wang, Libing Liu, Ruiwen Li, Jinfeng Wang, Qi Fu, Wanzhe Yuan

Published in: Archives of Virology | Issue 4/2016

Abstract

A novel recombinase polymerase amplification (RPA)-based method for detection of canine parvovirus type 2 (CPV-2) was developed. Sensitivity analysis showed that the detection limit of RPA was 10 copies of CPV-2 genomic DNA. RPA amplified both CPV-2a and -2b DNA but did not amplify the template of other important dog viruses (CCoV, PRV or CDV), demonstrating high specificity. The method was further validated with 57 canine fecal samples. An outstanding advantage of RPA is that it is an isothermal reaction and can be performed in a water bath, making RPA a potential alternative method for CPV-2 detection in resource-limited settings.

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Title: Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification
Authors: Jianchang Wang
Libing Liu
Ruiwen Li
Jinfeng Wang
Qi Fu
Wanzhe Yuan
Publication date: 01-04-2016
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 4/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2738-y

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