Top

Published in:

01-03-2016 | Brief Report

High-yield production of canine parvovirus virus-like particles in a baculovirus expression system

Authors: Hongli Jin, Xiaohong Xia, Bing Liu, Yu Fu, Xianping Chen, Huihui Wang, Zhenqiang Xia

Published in: Archives of Virology | Issue 3/2016

Abstract

An optimized VP2 gene from the current prevalent CPV strain (new CPV-2a) in China was expressed in a baculovirus expression system. It was found that the VP2 proteins assembled into virus-like particles (VLPs) with antigenic properties similar to those of natural CPV and with an especially high hemagglutination (HA) titer (1:2²⁰). Dogs intramuscularly or orally immunized with VLPs produced antibodies against CPV with >1:80 hemagglutination inhibition (HI) units for at least 3 months. The CPV VLPs could be considered for use as a vaccine against CPV or as a platform for research on chimeric VLP vaccines against other diseases.

Airenne KJ, Hu YC, Kost TA, Smith RH, Kotin RM, Ono C, Matsuura Y, Wang S, Yla-Herttuala S (2013) Baculovirus: an insect-derived vector for diverse gene transfer applications. Mol Ther 21:739–749PubMedCentralCrossRefPubMed

Buonavoglia C, Compagnucci M, Orfei Z (1983) Dog response to plaque variant of canine parvovirus. Zentralbl Veterinarmed B 30:526–531CrossRefPubMed

Calderon MG, Mattion N, Bucafusco D, Fogel F, Remorini P, La Torre J (2009) Molecular characterization of canine parvovirus strains in Argentina: detection of the pathogenic variant CPV2c in vaccinated dogs. J Virol Methods 159:141–145CrossRefPubMed

Casal JI (1999) Use of parvovirus-like particles for vaccination and induction of multiple immune responses. Biotechnol Appl Biochem 29(Pt 2):141–150PubMed

Cavalli A, Bozzo G, Decaro N, Tinelli A, Aliberti A, Buonavoglia D (2001) Characterization of a canine parvovirus strain isolated from an adult dog. New Microbiol 24:239–242PubMed

Cavalli A, Martella V, Desario C, Camero M, Bellacicco AL, De Palo P, Decaro N, Elia G, Buonavoglia C (2008) Evaluation of the antigenic relationships among canine parvovirus type 2 variants. Clin Vaccine Immunol 15:534–539PubMedCentralCrossRefPubMed

Crisci E, Barcena J, Montoya M (2012) Virus-like particles: the new frontier of vaccines for animal viral infections. Vet Immunol Immunopathol 148:211–225CrossRefPubMed

Decaro N, Desario C, Elia G, Martella V, Mari V, Lavazza A, Nardi M, Buonavoglia C (2008) Evidence for immunisation failure in vaccinated adult dogs infected with canine parvovirus type 2c. New Microbiol 31:125–130PubMed

Elia G, Desario C, Pezzoni G, Camero M, Brocchi E, Decaro N, Martella V, Buonavoglia C (2012) Recombinant ELISA using baculovirus-expressed VP2 for detection of antibodies against canine parvovirus. J Virol Methods 184:98–102CrossRefPubMed

10.

Feng H, Hu GQ, Wang HL, Liang M, Liang H, Guo H, Zhao P, Yang YJ, Zheng XX, Zhang ZF, Zhao YK, Gao YW, Yang ST, Xia XZ (2014) Canine parvovirus VP2 protein expressed in silkworm pupae self-assembles into virus-like particles with high immunogenicity. PLoS One 9:e79575PubMedCentralCrossRefPubMed

11.

Gil F, Brun A, Wigdorovitz A, Catala R, Martinez-Torrecuadrada JL, Casal I, Salinas J, Borca MV, Escribano JM (2001) High-yield expression of a viral peptide vaccine in transgenic plants. FEBS Lett 488:13–17CrossRefPubMed

12.

Gil F, Reytor E, Perez-Filgueira DM, Escribano JM (2007) Multimerization of peptide antigens for production of stable immunogens in transgenic plants. J Biotechnol 128:512–518CrossRefPubMed

13.

Graf M, Bojak A, Deml L, Bieler K, Wolf H, Wagner R (2000) Concerted action of multiple cis-acting sequences is required for Rev dependence of late human immunodeficiency virus type 1 gene expression. J Virol 74:10822–10826PubMedCentralCrossRefPubMed

14.

Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias and heterologous protein expression. Trends Biotechnol 22:346–353CrossRefPubMed

15.

Hu YC, Yao K, Wu TY (2008) Baculovirus as an expression and/or delivery vehicle for vaccine antigens. Expert Rev Vaccines 7:363–371CrossRefPubMed

16.

Ikemura T (1981) Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes. J Mol Biol 146:1–21CrossRefPubMed

17.

Luckow VA (1993) Baculovirus systems for the expression of human gene products. Curr Opin Biotechnol 4:564–572CrossRefPubMed

18.

Madhan S, Prabakaran M, Kwang J (2010) Baculovirus as vaccine vectors. Curr Gene Ther 10:201–213CrossRefPubMed

19.

Martella V, Cavalli A, Decaro N, Elia G, Desario C, Campolo M, Bozzo G, Tarsitano E, Buonavoglia C (2005) Immunogenicity of an intranasally administered modified live canine parvovirus type 2b vaccine in pups with maternally derived antibodies. Clin Diagn Lab Immunol 12:1243–1245PubMedCentralPubMed

20.

Pollock RV, Carmichael LE (1982) Maternally derived immunity to canine parvovirus infection: transfer, decline, and interference with vaccination. J Am Vet Med Assoc 180:37–42PubMed

21.

Pollock RV, Carmichael LE (1982) Dog response to inactivated canine parvovirus and feline panleukopenia virus vaccines. Cornell Vet 72:16–35PubMed

22.

Pratelli A, Cavalli A, Martella V, Tempesta M, Decaro N, Carmichael LE, Buonavoglia C (2001) Canine parvovirus (CPV) vaccination: comparison of neutralizing antibody responses in pups after inoculation with CPV2 or CPV2b modified live virus vaccine. Clin Diagn Lab Immunol 8:612–615PubMedCentralPubMed

23.

Saliki JT, Mizak B, Flore HP, Gettig RR, Burand JP, Carmichael LE, Wood HA, Parrish CR (1992) Canine parvovirus empty capsids produced by expression in a baculovirus vector: use in analysis of viral properties and immunization of dogs. J Gen Virol 73(Pt 2):369–374CrossRefPubMed

24.

Tokuoka M, Tanaka M, Ono K, Takagi S, Shintani T, Gomi K (2008) Codon optimization increases steady-state mRNA levels in Aspergillus oryzae heterologous gene expression. Appl Environ Microbiol 74:6538–6546PubMedCentralCrossRefPubMed

25.

Truyen U (2006) Evolution of canine parvovirus—a need for new vaccines? Vet Microbiol 117:9–13CrossRefPubMed

26.

Yi L, Tong M, Cheng Y, Song W, Cheng S (2014) Phylogenetic analysis of canine parvovirus VP2 gene in China. Transbound Emerg Dis. doi:10.1111/tbed.12268

27.

Yuan W, Parrish CR (2001) Canine parvovirus capsid assembly and differences in mammalian and insect cells. Virology 279:546–557CrossRefPubMed

28.

Zhang R, Yang S, Zhang W, Zhang T, Xie Z, Feng H, Wang S, Xia X (2010) Phylogenetic analysis of the VP2 gene of canine parvoviruses circulating in China. Virus Genes 40:397–402CrossRefPubMed

29.

Zhi N, Wan Z, Liu X, Wong S, Kim DJ, Young NS, Kajigaya S (2010) Codon optimization of human parvovirus B19 capsid genes greatly increases their expression in nonpermissive cells. J Virol 84:13059–13062PubMedCentralCrossRefPubMed

Title: High-yield production of canine parvovirus virus-like particles in a baculovirus expression system
Authors: Hongli Jin
Xiaohong Xia
Bing Liu
Yu Fu
Xianping Chen
Huihui Wang
Zhenqiang Xia
Publication date: 01-03-2016
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 3/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2719-1

ACC 2024 Congress Coverage

Heart Failure Video

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

High-yield production of canine parvovirus virus-like particles in a baculovirus expression system

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2016

Molecular characterization of infectious bursal disease viruses detected in vaccinated commercial broiler flocks in Lusaka, Zambia

Generation and evaluation of a chimeric classical swine fever virus expressing a visible marker gene

Nucleotide sequence analysis of Vietnamese highly pathogenic porcine reproductive and respiratory syndrome virus from 2013 to 2014 based on the NSP2 and ORF5 coding regions

Immunoenhancement with flagellin as an adjuvant to whole-killed rabies vaccine in mice

Phylogenetic relationships of Iranian infectious hematopoietic necrosis virus of rainbow trout (Oncorhynchus mykiss) based on the glycoprotein gene

A report on the outbreak of Zika virus on Easter Island, South Pacific, 2014

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page