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01-02-2016 | Original Article

Characterization of the novel T4-like Salmonella enterica bacteriophage STP4-a and its endolysin

Authors: Meng Li, Mengzhe Li, Hong Lin, Jingxue Wang, Yanqiu Jin, Feng Han

Published in: Archives of Virology | Issue 2/2016

Abstract

While screening for new antimicrobial agents for multidrug-resistant Salmonella enterica, the novel lytic bacteriophage STP4-a was isolated and characterized. Phage morphology revealed that STP4-a belongs to the family Myoviridae. Bacterial challenge assays showed that different serovars of Salmonella enterica were susceptible to STP4-a infection. The genomic characteristics of STP4-a, containing 159,914 bp of dsDNA with an average GC content of 36.86 %, were determined. Furthermore, the endolysin of STP4-a was expressed and characterized. The novel endolysin, LysSTP4, has hydrolytic activity towards outer-membrane-permeabilized S. enterica and Escherichia coli. These results provide essential information for the development of novel phage-based biocontrol agents against S. enterica.

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Title: Characterization of the novel T4-like Salmonella enterica bacteriophage STP4-a and its endolysin
Authors: Meng Li
Mengzhe Li
Hong Lin
Jingxue Wang
Yanqiu Jin
Feng Han
Publication date: 01-02-2016
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 2/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2647-0

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