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Archives of Virology
Published in: Archives of Virology 2/2016

01-02-2016 | Brief Report

Clinical validation of a novel real-time human papillomavirus assay for simultaneous detection of 14 high-risk HPV type and genotyping HPV type 16 and 18 in China

Authors: Hui Yang, Lie-Jun Li, Long-Xu Xie, Zhao-Yun Luo, Min Lu, Min Lin, Xiang-bin Zheng, Yue Huang, Li-Ye Yang

Published in: Archives of Virology | Issue 2/2016

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Abstract

In the present study, we describe the laboratory workflow and the clinical validation of a novel multiplex real-time PCR-based HPV assay in China. The cross-sectional validation analysis showed that this assay worked well for detection of 14 HR-HPV types and identification of HPV 16 and 18 in a single sensitive assay that is suitable for both clinical usage and high-throughput cervical screening purposes. We predict that this accurate, high-throughput and low-cost HPV assay can greatly reduce the heavy economic burden of HPV detection in China.
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Metadata
Title
Clinical validation of a novel real-time human papillomavirus assay for simultaneous detection of 14 high-risk HPV type and genotyping HPV type 16 and 18 in China
Authors
Hui Yang
Lie-Jun Li
Long-Xu Xie
Zhao-Yun Luo
Min Lu
Min Lin
Xiang-bin Zheng
Yue Huang
Li-Ye Yang
Publication date
01-02-2016
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 2/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2673-y

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