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Archives of Virology
Published in: Archives of Virology 11/2015

01-11-2015 | Review

Detection and genetic variation analysis of grapevine fanleaf virus (GFLV) isolates in China

Authors: Jun Zhou, Xudong Fan, Yafeng Dong, Zun ping Zhang, Fang Ren, Guojun Hu

Published in: Archives of Virology | Issue 11/2015

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Abstract

To investigate the prevalence and genetic variation of grapevine fanleaf virus (GFLV) in China, 142 grapevine samples from 13 provinces and regions were tested using DAS-ELISA, RT-PCR, and nested RT-PCR. Of the samples, 38 % tested positive for GFLV by DAS-ELISA, and 26.8 % tested positive by RT-PCR and nested RT-PCR. Movement protein (MP) and coat protein (CP) gene PCR products were cloned and sequenced. The MP or CP nucleotide and protein sequences shared identities that ranged from 94.9 % to 100 %. Phylogenetic analysis revealed that Chinese GFLV isolates obtained in this study were distinct from the isolates reported in GenBank.
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Metadata
Title
Detection and genetic variation analysis of grapevine fanleaf virus (GFLV) isolates in China
Authors
Jun Zhou
Xudong Fan
Yafeng Dong
Zun ping Zhang
Fang Ren
Guojun Hu
Publication date
01-11-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 11/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2566-0

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