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Archives of Virology
Published in: Archives of Virology 9/2015

01-09-2015 | Original Article

Distribution and phylogenetic analysis of Culex flavivirus in mosquitoes in China

Authors: Wenkai Liang, Xiaoxia He, Guifang Liu, Shaobai Zhang, Shihong Fu, Min Wang, Weixin Chen, Ying He, Xiaoyan Tao, Hongyue Jiang, Xiaojuan Lin, Xiaoyan Gao, Weijun Hu, Yan Liu, Lei Feng, Yuxi Cao, Guang Yang, Chunxia Jing, Guodong Liang, Huanyu Wang

Published in: Archives of Virology | Issue 9/2015

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Abstract

Culex flavivirus (CxFV) is an insect-specific virus of the genus Flavivirus. CxFV strains have been isolated from Cx. pipiens, Cx. quinquefasciatus, and other Cx. species in Asia, Africa, North America, Central America and South America. CxFV was isolated for the first time in China in 2006. As this is a novel flavivirus, we explored the distribution and genetic characteristics of Culex flavivirus in China. A total of 46,649 mosquitoes were collected in seven provinces between 2004 and 2012 and were analysed in 871 pools. 29 CxFV RNAs from Cx. pipiens, Cx. tritaeniorhynchus, Anopheles Sinensis, and Culex spp. tested positive for CxFV in real-time RT-PCR. 6 CxFV strains were isolated from Cx. species collected in Shandong, Henan, and Shaanxi provinces, while no virus or viral RNA was detected in samples from Sichuan, Chongqing, Hubei, and Fujian. Phylogenetic analysis of the envelope gene indicated that Chinese strains formed a robust subgroup of genotype 1, together with viruses from the United States and Japan. This study demonstrates that the geographic distribution of CxFV in China is widespread, but geographical boundaries to spread are apparent. Our findings suggest that CxFV can infect various mosquito species in nature.
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Metadata
Title
Distribution and phylogenetic analysis of Culex flavivirus in mosquitoes in China
Authors
Wenkai Liang
Xiaoxia He
Guifang Liu
Shaobai Zhang
Shihong Fu
Min Wang
Weixin Chen
Ying He
Xiaoyan Tao
Hongyue Jiang
Xiaojuan Lin
Xiaoyan Gao
Weijun Hu
Yan Liu
Lei Feng
Yuxi Cao
Guang Yang
Chunxia Jing
Guodong Liang
Huanyu Wang
Publication date
01-09-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 9/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2492-1

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