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01-09-2015 | Original Article

Construction and characterization of a recombinant reticuloendotheliosis virus expressing enhanced green fluorescent protein

Authors: Xiaoyun Deng, Feng Hu, Xiaole Qi, Li Gao, Kai Li, Honglei Gao, Yulong Gao, Yongqiang Wang, Nan Shen, Yuping Hua, Xiaomei Wang

Published in: Archives of Virology | Issue 9/2015

Abstract

Reticuloendotheliosis virus (REV) causes an immunosuppressive and oncogenic disease in chickens and other birds. In this study, based on an infectious clone of REV, named HLJR0901, a recombinant virus containing the enhanced green fluorescence protein (EGFP) gene was constructed by inserting the EGFP expression cassette downstream of the 3′ terminus of the viral env gene. An EGFP-tagged REV that stably expresses EGFP was rescued. This visible recombinant REV could contribute to the further understanding of the molecular mechanism involved in the replication and pathogenicity of REV.

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Title: Construction and characterization of a recombinant reticuloendotheliosis virus expressing enhanced green fluorescent protein
Authors: Xiaoyun Deng
Feng Hu
Xiaole Qi
Li Gao
Kai Li
Honglei Gao
Yulong Gao
Yongqiang Wang
Nan Shen
Yuping Hua
Xiaomei Wang
Publication date: 01-09-2015
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 9/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-015-2502-3

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