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Archives of Virology
Published in: Archives of Virology 6/2015

01-06-2015 | Original Article

Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth

Authors: Zhiqiang Zhang, Wencheng Lin, Xiaoqi Li, Hong Cao, Yongqiang Wang, Shijun J. Zheng

Published in: Archives of Virology | Issue 6/2015

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Abstract

Avian reovirus (ARV) causes viral arthritis, chronic respiratory diseases, retarded growth and malabsorption syndrome. It is well established that the ARV sigma-C protein induces apoptosis in host cells. However, the underlying molecular mechanism of this induction is still unclear. We report here the identification of eukaryotic elongation factor 1 alpha 1 (EEF1A1) as the interacting partner of σC. We found that σC-induced apoptosis in DF-1 cells could be completely abolished by knockdown of EEF1A1 by siRNA. Furthermore, knockdown of EEF1A1 markedly reduced ARV-induced apoptosis associated with decreased caspase-9 and -3 activation and cytochrome C release, leading to increased ARV growth in host cells. Thus, EEF1A1 plays a critical role in σC-induced apoptosis and inhibition of viral growth.
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Metadata
Title
Critical role of eukaryotic elongation factor 1 alpha 1 (EEF1A1) in avian reovirus sigma-C-induced apoptosis and inhibition of viral growth
Authors
Zhiqiang Zhang
Wencheng Lin
Xiaoqi Li
Hong Cao
Yongqiang Wang
Shijun J. Zheng
Publication date
01-06-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 6/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2403-5

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