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Archives of Virology
Published in: Archives of Virology 6/2015

01-06-2015 | Brief Report

Adsorption of a porcine reproductive and respiratory syndrome virus DNA vaccine candidate onto biodegradable nanoparticles improves immunogenicity in mice

Authors: Luping Du, Bin Li, Xiangwei Xu, Bing Sun, Fengjiao Pang, Libin Wen, Kehe Huang, Kongwang He

Published in: Archives of Virology | Issue 6/2015

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Abstract

Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating viral disease of pigs. Safer and more effective vaccines are urgently needed. In this study, a synthetic ORF5 gene of porcine reproductive and respiratory syndrome virus (PRRSV) was adsorbed onto poly(D, L-lactide-co-glycolide)/polyethylenimine (PLGA/PEI) nanoparticles. We prepared a PLGA-nanoparticle-adsorbed PRRSV DNA vaccine and a PEI-DNA complex. The results showed that these model vaccines could significantly enhance humoral and cellular immune responses when compared with the responses induced by pcDNA3.1-SynORF5, a plasmid construct for expression of PRRSV ORF5. PLGA-branched PEI nanoparticles induced the most efficient immune response. The delivery system and adjuvant provide new models for the development of vaccines against PRRSV.
Literature
1.
Ai H, Jones SA, de Villiers MM, Lvov YM (2003) Nano-encapsulation of furosemide microcrystals for controlled drug release. J Control Release 86:59–68CrossRefPubMed
2.
Bachelder EM, Beaudette TT, Broaders KE, Frechet JM, Albrecht MT, Mateczun AJ, Ainslie KM, Pesce JT, Keane-Myers AM (2010) In vitro analysis of acetalated dextran microparticles as a potent delivery platform for vaccine adjuvants. Mol Pharm 7:826–835CrossRefPubMedCentralPubMed
3.
Binjawadagi B, Dwivedi V, Manickam C, Ouyang K, Torrelles JB, Renukaradhya GJ (2014) An innovative approach to induce cross-protective immunity against porcine reproductive and respiratory syndrome virus in the lungs of pigs through adjuvanted nanotechnology-based vaccination. Int J Nanomedicine 9:1519–1535PubMedCentralPubMed
4.
Binjawadagi B, Dwivedi V, Manickam C, Ouyang K, Wu Y, Lee LJ, Torrelles JB, Renukaradhya GJ (2014) Adjuvanted poly(lactic-co-glycolic) acid nanoparticle-entrapped inactivated porcine reproductive and respiratory syndrome virus vaccine elicits cross-protective immune response in pigs. Int J Nanomedicine 9:679–694PubMedCentralPubMed
5.
Cao X, Shoichet MS (1999) Delivering neuroactive molecules from biodegradable microspheres for application in central nervous system disorders. Biomaterials 20:329–339CrossRefPubMed
6.
Capan Y, Woo BH, Gebrekidan S, Ahmed S, DeLuca PP (1999) Preparation and characterization of poly (D, L-lactide-co-glycolide) microspheres for controlled release of poly(L-lysine) complexed plasmid DNA. Pharm Res 16:509–513CrossRefPubMed
7.
Demento SL, Bonafe N, Cui W, Kaech SM, Caplan MJ, Fikrig E, Ledizet M, Fahmy TM (2010) TLR9-targeted biodegradable nanoparticles as immunization vectors protect against West Nile encephalitis. J Immunol 185:2989–2997CrossRefPubMedCentralPubMed
8.
Du L, Li B, He K, Zhang H, Huang K, Xiao S (2013) Construction and immunogenicity of DNA vaccines encoding fusion protein of porcine IFN- lambda 1 and GP5 gene of porcine reproductive and respiratory syndrome virus. Biomed Res Int 2013:318698PubMedCentralPubMed
9.
Dwivedi V, Manickam C, Binjawadagi B, Joyappa D, Renukaradhya GJ (2012) Biodegradable nanoparticle-entrapped vaccine induces cross-protective immune response against a virulent heterologous respiratory viral infection in pigs. PLoS One 7:e51794CrossRefPubMedCentralPubMed
10.
Dwivedi V, Manickam C, Binjawadagi B, Renukaradhya GJ (2013) PLGA nanoparticle entrapped killed porcine reproductive and respiratory syndrome virus vaccine helps in viral clearance in pigs. Vet Microbiol 166:47–58CrossRefPubMed
11.
Eldridge JH, Gilley RM, Staas JK, Moldoveanu Z, Meulbroek JA, Tice TR (1989) Biodegradable microspheres: vaccine delivery system for oral immunization. Curr Top Microbiol Immunol 146:59–66PubMed
12.
Fang L, Jiang Y, Xiao S, Niu C, Zhang H, Chen H (2006) Enhanced immunogenicity of the modified GP5 of porcine reproductive and respiratory syndrome virus. Virus Genes 32:5–11CrossRefPubMed
13.
Garzon MR, Berraondo P, Crettaz J, Ochoa L, Vera M, Lasarte JJ, Vales A, Van Rooijen N, Ruiz J, Prieto J, Zulueta J, Gonzalez-Aseguinolaza G (2005) Induction of gp120-specific protective immune responses by genetic vaccination with linear polyethylenimine-plasmid complex. Vaccine 23:1384–1392CrossRefPubMed
14.
Gupta RK, Chang AC, Siber GR (1998) Biodegradable polymer microspheres as vaccine adjuvants and delivery systems. Dev Biol Stand 92:63–78PubMed
15.
Kasturi SP, Sachaphibulkij K, Roy K (2005) Covalent conjugation of polyethyleneimine on biodegradable microparticles for delivery of plasmid DNA vaccines. Biomaterials 26:6375–6385CrossRefPubMed
16.
Kim WJ, Chang CW, Lee M, Kim SW (2007) Efficient siRNA delivery using water soluble lipopolymer for anti-angiogenic gene therapy. J Control Release 118:357–363CrossRefPubMed
17.
Li B, Xiao S, Wang Y, Xu S, Jiang Y, Chen H, Fang L (2009) Immunogenicity of the highly pathogenic porcine reproductive and respiratory syndrome virus GP5 protein encoded by a synthetic ORF5 gene. Vaccine 27:1957–1963CrossRefPubMed
18.
Lu JM, Wang X, Marin-Muller C, Wang H, Lin PH, Yao Q, Chen C (2009) Current advances in research and clinical applications of PLGA-based nanotechnology. Expert Rev Mol Diagn 9:325–341CrossRefPubMedCentralPubMed
19.
Meulenberg JJ, Petersen-den Besten A, De Kluyver EP, Moormann RJ, Schaaper WM, Wensvoort G (1995) Characterization of proteins encoded by ORFs 2 to 7 of Lelystad virus. Virology 206:155–163CrossRefPubMed
20.
Mok H, Park TG (2008) Direct plasmid DNA encapsulation within PLGA nanospheres by single oil-in-water emulsion method. Eur J Pharm Biopharm 68:105–111CrossRefPubMed
21.
Murtaugh MP, Elam MR, Kakach LT (1995) Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus. Arch Virol 140:1451–1460CrossRefPubMed
22.
Oster CG, Kim N, Grode L, Barbu-Tudoran L, Schaper AK, Kaufmann SH, Kissel T (2005) Cationic microparticles consisting of poly(lactide-co-glycolide) and polyethylenimine as carriers systems for parental DNA vaccination. J Control Release 104:359–377CrossRefPubMed
23.
Ostrowski M, Galeota JA, Jar AM, Platt KB, Osorio FA, Lopez OJ (2002) Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. J Virol 76:4241–4250CrossRefPubMedCentralPubMed
24.
Ren JQ, Sun WC, Lu HJ, Wen SB, Jing J, Yan FL, Liu H, Liu CX, Xiao PP, Chen X, Du SW, Du R, Jin NY (2014) Construction and immunogenicity of a DNA vaccine coexpressing GP3 and GP5 of genotype-I porcine reproductive and respiratory syndrome virus. BMC Vet Res 10:128CrossRefPubMedCentralPubMed
25.
Shau MD, Shih MF, Lin CC, Chuang IC, Hung WC, Hennink WE, Cherng JY (2012) A one-step process in preparation of cationic nanoparticles with poly(lactide-co-glycolide)-containing polyethylenimine gives efficient gene delivery. Eur J Pharm Sci 46:522–529CrossRefPubMed
26.
27.
Son S, Kim WJ (2010) Biodegradable nanoparticles modified by branched polyethylenimine for plasmid DNA delivery. Biomaterials 31:133–143CrossRefPubMed
28.
Spiers ID, Eyles JE, Baillie LW, Williamson ED, Alpar HO (2000) Biodegradable microparticles with different release profiles: effect on the immune response after a single administration via intranasal and intramuscular routes. J Pharm Pharmacol 52:1195–1201CrossRefPubMed
29.
Tai W, Roberts L, Seryshev A, Gubatan JM, Bland CS, Zabriskie R, Kulkarni S, Soong L, Mbawuike I, Gilbert B, Kheradmand F, Corry DB (2011) Multistrain influenza protection induced by a nanoparticulate mucosal immunotherapeutic. Mucosal Immunol 4:197–207CrossRefPubMed
30.
Thomas C, Rawat A, Hope-Weeks L, Ahsan F (2011) Aerosolized PLA and PLGA nanoparticles enhance humoral, mucosal and cytokine responses to hepatitis B vaccine. Mol Pharm 8:405–415CrossRefPubMed
31.
Xiao S, Chen H, Fang L, Liu C, Zhang H, Jiang Y, Hong W (2004) Comparison of immune responses and protective efficacy of suicidal DNA vaccine and conventional DNA vaccine encoding glycoprotein C of pseudorabies virus in mice. Vaccine 22:345–351CrossRefPubMed
Metadata
Title
Adsorption of a porcine reproductive and respiratory syndrome virus DNA vaccine candidate onto biodegradable nanoparticles improves immunogenicity in mice
Authors
Luping Du
Bin Li
Xiangwei Xu
Bing Sun
Fengjiao Pang
Libin Wen
Kehe Huang
Kongwang He
Publication date
01-06-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 6/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-015-2396-0

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Keynote webinar | Spotlight on medication adherence

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WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

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