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Archives of Virology
Published in: Archives of Virology 2/2015

01-02-2015 | Brief Report

Genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus Tospovirus

Authors: Pei-Ru Wu, Wan-Chu Chien, Mitsuru Okuda, Minoru Takeshita, Shyi-Dong Yeh, Yun-Chi Wang, Tsung-Chi Chen

Published in: Archives of Virology | Issue 2/2015

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Abstract

Chrysanthemum stem necrosis virus (CSNV) is a member of a tentative tospovirus species. In this study, the complete genomic sequence of the Japanese CSNV isolate TcCh07A was determined. The L RNA is 8960 nt long and encodes the 331.0-kDa RNA-dependent RNA polymerase. The M RNA is 4828 nt long and encodes the 34.1-kDa movement protein (NSm) and the 127.7-kDa glycoprotein precursor (Gn/Gc). The S RNA is 2949 nt long and encodes the 52.4-kDa silencing suppressor protein (NSs) and the 29.3-kDa nucleocapsid (N) protein. The N protein of CSNV-TcCh07A was purified from virus-infected plant tissues and used for production of a rabbit polyclonal antiserum (RAs) and a monoclonal antibody (MAb). Results of serological tests by indirect ELISA and western blotting using the prepared RAs and MAb and a previously produced RAs against the N protein of tomato spotted wilt virus (TSWV) indicated that CSNV-TcCh07A, TSWV, tomato chlorotic spot virus, groundnut ringspot virus, alstroemeria necrotic streak virus and impatiens necrotic spot virus are serologically related.
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Metadata
Title
Genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus Tospovirus
Authors
Pei-Ru Wu
Wan-Chu Chien
Mitsuru Okuda
Minoru Takeshita
Shyi-Dong Yeh
Yun-Chi Wang
Tsung-Chi Chen
Publication date
01-02-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 2/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-014-2287-9

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