Top

Published in:

01-02-2015 | Brief Report

Full-length genome sequence analysis of a Hungarian porcine reproductive and respiratory syndrome virus isolated from a pig with severe respiratory disease

Authors: Ádám Bálint, Gyula Balka, Péter Horváth, Sándor Kecskeméti, Ádám Dán, Attila Farsang, Levente Szeredi, Krisztián Bányai, Dániel Bartha, Ferenc Olasz, Sándor Belák, Zoltán Zádori

Published in: Archives of Virology | Issue 2/2015

Abstract

Here, we report the isolation of a type 1 porcine reproductive and respiratory syndrome virus (PRRSV) strain from a clinical outbreak of severe respiratory problems and high fever. Next-generation sequencing was used to determine the complete genome sequence of the isolate (9625/2012). The virus belongs to a new branch within subtype 1, clade D, and shows the highest similarity to PRRSV Olot/1991 and to the Amervac vaccine strain. Mutation analysis of 9625/2012 revealed no evidence of recombination but did show a high proportion of amino acid substitutions in the putative neutralizing epitopes, suggesting an important role of selective immune pressure in the evolution of PRRSV 9625/2012.

Meulenberg JJ, Hulst MM, de Meijer EJ, Moonen PL, den Besten A, de Kluyver EP, Wensvoort G, Moormann RJ (1993) Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192:62–72PubMedCrossRef

Shi M, Lam TT, Hon CC, Murtaugh MP, Davies PR, Hui RK, Li J, Wong LT, Yip CW, Jiang JW, Leung FC (2010) Phylogeny-based evolutionary, demographical, and geographical dissection of North American type 2 porcine reproductive and respiratory syndrome viruses. J Virol 84:8700–8711PubMedCentralPubMedCrossRef

Stadejek T, Oleksiewicz MB, Potapchuk D, Podgórska K (2006) Porcine reproductive and respiratory syndrome virus strains of exceptional diversity in eastern Europe support the definition of new genetic subtypes. J Gen Virol 87:1835–1841PubMedCrossRef

Tian K, Yu X, Zhao T, Feng Y, Cao Z, Wang C, Hu Y, Chen X, Hu D, Tian X et al (2007) Emergence of fatal PRRSV Variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PLoS ONE 2:e526PubMedCentralPubMedCrossRef

Karniychuk UU, Geldhof M, Vanhee M, van Doorsselaere J, Saveleva TA, Nauwynck HJ (2010) Pathogenesis and antigenic characterization of a new East European subtype 3 porcine reproductive and respiratory syndrome virus isolate. BMC Vet Res 6:30PubMedCentralPubMedCrossRef

Morgan SB, Graham SP, Salguero FJ, Sánchez Cordón PJ, Mokhtar H, Rebel JM, Weesendorp E, Bodman-Smith KB, Steinbach F, Frossard JP (2013) Increased pathogenicity of European porcine reproductive and respiratory syndrome virus is associated with enhanced adaptive responses and viral clearance. Vet Microbiol 163:13–22PubMedCrossRef

Beura LK, Subramaniam S, Vu HL, Kwon B, Pattnaik AK, Osorio FA (2012) Identification of amino acid residues important for anti-IFN activity of porcine reproductive and respiratory syndrome virus non-structural protein 1. Virology 433:431–439PubMedCrossRef

Subramaniam S, Beura LK, Kwon B, Pattnaik AK, Osorio FA (2012) Amino acid residues in the non-structural protein 1 of porcine reproductive and respiratory syndrome virus involved in down-regulation of TNF-α expression in vitro and attenuation in vivo. Virology 432:241–249PubMedCrossRef

Oleksiewicz MB, Bøtner A, Normann P (2002) Porcine B-cells recognize epitopes that are conserved between the structural proteins of American- and European-type porcine reproductive and respiratory syndrome virus. J Gen Virol 83:1407–1418PubMed

10.

Ostrowski M, Galeota JA, Jar AM, Platt KB, Osorio FA, Lopez OJ (2002) Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. J Virol 76:4241–4250PubMedCentralPubMedCrossRef

11.

Díaz I, Pujols J, Ganges L, Gimeno M, Darwich L, Domingo M, Mateu E (2009) In silico prediction and ex vivo evaluation of potential T-cell epitopes in glycoproteins 4 and 5 and nucleocapsid protein of genotype-I (European) of porcine reproductive and respiratory syndrome virus. Vaccine 27:5603–5611PubMedCrossRef

12.

Balka G, Hornyák A, Bálint A, Benyeda Z, Rusvai M (2009) Development of a one-step real-time quantitative PCR assay based on primer-probe energy transfer for the detection of porcine reproductive and respiratory syndrome virus. J Virol Meth 158:41–45CrossRef

13.

Balka G, Hornyák A, Bálint A, Kiss I, Kecskeméti S, Bakonyi T, Rusvai M (2008) Genetic diversity of porcine reproductive and respiratory syndrome virus strains circulating in Hungarian swine herds. Vet Microbiol 127:128–135PubMedCrossRef

14.

Manual of diagnostic tests and vaccines for terrestrial animals (2013) Office International des Epizooties (Paris). http://www.oie.int/international-standard-setting/terrestrial-manual/access-online/

15.

Stemke GW, Phan R, Young TF, Ross RF (1994) Differentiation of Mycoplasma hyopneumoniae, M. flocculare, and M. hyorhinis on the basis of amplification of a 16S rRNA gene sequence. Am J Vet Res 55:81–84PubMed

16.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCentralPubMedCrossRef

17.

Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarni SS, Novak NG, Ingersoll R, Sheppard HW, Ray SC (1999) Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 73:152–160PubMedCentralPubMed

18.

Gupta R, Brunak S (2002) Prediction of N-glycosylation sites in human proteins. Pac Symp Biocomput 7:310–322

19.

Vanhee M, Van Breedam W, Costers S, Geldhof M, Noppe Y, Nauwynck H (2011) Characterization of antigenic regions in the porcine reproductive and respiratory syndrome virus by the use of peptide-specific serum antibodies. Vaccine 29:4794–4804PubMedCrossRef

20.

Oleksiewicz MB, Bøtner A, Toft P, Grubbe T, Nielsen J, Kamstrup S, Storgaard T (2000) Emergence of porcine reproductive and respiratory syndrome virus deletion mutants: correlation with the porcine antibody response to a hypervariable site in the ORF 3 structural glycoprotein. Virology 267:135–140PubMedCrossRef

21.

Meulenberg JJ, van Nieuwstadt AP, van Essen-Zandbergen A, Langeveld JP (1997) Posttranslational processing and identification of a neutralization domain of the GP4 protein encoded by ORF4 of the Lelystad Virus. J Virol 71:6061–6070PubMedCentralPubMed

22.

Wissink EH, van Wijk HA, Kroese MV, Weiland E, Meulenberg JJ, Rottier PJ, van Rijn PA (2003) The major envelope protein, GP5, of a European porcine reproductive and respiratory syndrome virus contains a neutralization epitope in its N-terminal ectodomain. J Gen Virol 84:1535–1543PubMedCrossRef

23.

Van Breedam W, Costers S, Vanhee M, Gagnon CA, Rodríguez-Gómez IM, Geldhof M, Verbeeck M, Van Doorsselaere J, Karniychuk U, Nauwynck HJ (2011) Porcine reproductive and respiratory syndrome virus (PRRSV)-specific mAbs: supporting diagnostics and providing new insights into the antigenic properties of the virus. Vet Immunol Immunopathol 141:246–257PubMedCrossRef

24.

Prieto C, Vázquez A, Núñez JI, Alvarez E, Simarro I, Castro JM (2009) Influence of time on the genetic heterogeneity of Spanish porcine reproductive and respiratory syndrome virus isolates. Vet J 180:363–370PubMedCrossRef

25.

Chen Z, Lawson S, Sun Z, Zhou X, Guan X, Christopher-Hennings J, Nelson EA, Fang Y (2010) Identification of two auto-cleavage products of nonstructural protein 1 (nsp1) in porcine reproductive and respiratory syndrome virus infected cells: nsp1 function as interferon antagonist. Virology 398:87–97PubMedCrossRef

26.

Yan Y, Guo X, Ge X, Chen Y, Cha Z, Yang H (2007) Monoclonal antibody and porcine antisera recognized B-cell epitopes of Nsp2 protein of a Chinese strain of porcine reproductive and respiratory syndrome virus. Virus Res 126:207–215PubMedCrossRef

27.

Gauger PC, Faaberg KS, Guo B, Kappes MA, Opriessnig T (2012) Genetic and phenotypic characterization of a 2006 United States porcine reproductive and respiratory virus isolate associated with high morbidity and mortality in the field. Virus Res 163:98–107PubMedCrossRef

28.

Cancel-Tirado SM, Evans RB, Yoon KJ (2004) Monoclonal antibody analysis of porcine reproductive and respiratory syndrome virus epitopes associated with antibody-dependent enhancement and neutralization of virus infection. Vet Immunol Immunopathol 102:249–262PubMedCrossRef

29.

Costers S, Vanhee M, Van Breedam W, Van Doorsselaere J, Geldhof M, Nauwynck HJ (2010) GP4-specific neutralizing antibodies might be a driving force in PRRSV evolution. Virus Res 154:104–113PubMedCrossRef

30.

Thacker EL, Halbur PG, Ross RF, Thanawongnuwech R, Thacker BJ (1999) Mycoplasma hyopneumoniae potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia. Microbiol 37:620–627

31.

Thanawongnuwech R, Thacker B, Halbur P, Thacker EL (2004) Increased production of proinflammatory cytokines following infection with porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae. Clin Diagn Lab Immunol 11:901–908PubMedCentralPubMed

Title: Full-length genome sequence analysis of a Hungarian porcine reproductive and respiratory syndrome virus isolated from a pig with severe respiratory disease
Authors: Ádám Bálint
Gyula Balka
Péter Horváth
Sándor Kecskeméti
Ádám Dán
Attila Farsang
Levente Szeredi
Krisztián Bányai
Dániel Bartha
Ferenc Olasz
Sándor Belák
Zoltán Zádori
Publication date: 01-02-2015
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 2/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-014-2265-2

Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin

Prof. Florian Limbourg

Prof. Anoop Chauhan

Developed by: Springer Medicine

Obesity Clinical Trial Summary

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2015

The N-linked glycosylation site at position 158 on the head of hemagglutinin and the virulence of H5N1 avian influenza virus in mice

The complete nucleotide sequence of chrysanthemum stem necrosis virus

Oysters as hot spots for mimivirus isolation

Mapping the distribution of maize streak virus genotypes across the forest and transition zones of Ghana

The first complete genome sequences of two distinct European tomato spotted wilt virus isolates

Phylogenetic analysis of a G6P[5] bovine rotavirus strain isolated in a neonatal diarrhea outbreak in a beef cattle herd vaccinated with G6P[1] and G10P[11] genotypes

Keynote webinar | Spotlight on medication adherence

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

At a glance: The STEP trials