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Published in: Archives of Virology 1/2015

01-01-2015 | Annotated Sequence Record

Recombination in truncated genome sequences of porcine circovirus type 2

Authors: Libin Wen, Fengzhi Wang, Kongwang He, Bin Li, Xiaomin Wang, Rongli Guo, Jianping Xie

Published in: Archives of Virology | Issue 1/2015

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Abstract

Porcine circovirus type 2 (PCV2) is the causal agent of a serious disease found in pigs. Here, we report the first detection of truncated genome sequences of PCV2 strain ZJ-R, with the genomic region encoding part of Rep and Cap with a nonviral insertion. To our knowledge, the genome of ZJ-R represents the first PCV2 DNA with a coding insertion. The PCV2 ZJ-R genome is 694 nucleotides long and has two main open reading frames. The whole genome sequence of ZJ-R may facilitate further study of the origin and evolution of PCV2.
Literature
1.
go back to reference Cadar D, Cságola A, Lorincz M, Tombácz K, Spînu M, Tuboly T (2012) Detection of natural inter- and intra-genotype recombination events revealed by cap gene analysis and decreasing prevalence of PCV2 in wild boars. Infect Genet Evol 12(2):420–427CrossRefPubMed Cadar D, Cságola A, Lorincz M, Tombácz K, Spînu M, Tuboly T (2012) Detection of natural inter- and intra-genotype recombination events revealed by cap gene analysis and decreasing prevalence of PCV2 in wild boars. Infect Genet Evol 12(2):420–427CrossRefPubMed
2.
go back to reference Cai L, Ni J, Xia Y, Zi Z, Ning K, Qiu P, Li X, Wang B, Liu Q, Hu D, Yu X, Zhou Z, Zhai X, Han X, Tian K (2012) Identification of an emerging recombinant cluster in porcine circovirus type 2. Virus Res 165(1):95–102CrossRefPubMed Cai L, Ni J, Xia Y, Zi Z, Ning K, Qiu P, Li X, Wang B, Liu Q, Hu D, Yu X, Zhou Z, Zhai X, Han X, Tian K (2012) Identification of an emerging recombinant cluster in porcine circovirus type 2. Virus Res 165(1):95–102CrossRefPubMed
3.
4.
go back to reference Duffy S, Shackelton LA, Holmes EC (2008) Rates of evolutionary change in viruses: patterns and determinants. Nat Rev Genet 9(4):267–276CrossRefPubMed Duffy S, Shackelton LA, Holmes EC (2008) Rates of evolutionary change in viruses: patterns and determinants. Nat Rev Genet 9(4):267–276CrossRefPubMed
5.
go back to reference Hamel AL, Lin LL, Nayar GP (1998) Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. J Virol 72(6):5262–5267PubMedCentralPubMed Hamel AL, Lin LL, Nayar GP (1998) Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. J Virol 72(6):5262–5267PubMedCentralPubMed
6.
go back to reference Harding JC (1996) Post-weaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation. In: Proceedings of the 27th Annual Meeting of the Western Canadian Association of Swine Veterinarians, Saskatoon, Saskatchewan, Canada, p 21 Harding JC (1996) Post-weaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation. In: Proceedings of the 27th Annual Meeting of the Western Canadian Association of Swine Veterinarians, Saskatoon, Saskatchewan, Canada, p 21
7.
go back to reference He J, Cao J, Zhou N, Jin Y, Wu J, Zhou J (2013) Identification and functional analysis of the novel ORF4 protein encoded by porcine circovirus type 2. J Virol 87(3):1420–1429PubMedCentralCrossRefPubMed He J, Cao J, Zhou N, Jin Y, Wu J, Zhou J (2013) Identification and functional analysis of the novel ORF4 protein encoded by porcine circovirus type 2. J Virol 87(3):1420–1429PubMedCentralCrossRefPubMed
8.
go back to reference Liu J, Chen I, Du Q, Chua H, Kwang J (2006) The ORF3 protein of porcine circovirus type 2 is involved in viral pathogenesis in vivo. J Virol 80:5065–5073PubMedCentralCrossRefPubMed Liu J, Chen I, Du Q, Chua H, Kwang J (2006) The ORF3 protein of porcine circovirus type 2 is involved in viral pathogenesis in vivo. J Virol 80:5065–5073PubMedCentralCrossRefPubMed
9.
go back to reference Liu J, Chen I, Kwang J (2005) Characterization of a previously unidentified viral protein in porcine circovirus type 2-infected cells and its role in virus-induced apoptosis. J Virol 79:8262–8274PubMedCentralCrossRefPubMed Liu J, Chen I, Kwang J (2005) Characterization of a previously unidentified viral protein in porcine circovirus type 2-infected cells and its role in virus-induced apoptosis. J Virol 79:8262–8274PubMedCentralCrossRefPubMed
10.
go back to reference Luo W, Zhao D, Yu XL, Ge M, Li RC, Jiang DL (2013) Small-sized circular genomes similar to genome of porcine circovirus 2. Genome Announc 1(2):e0009313CrossRefPubMed Luo W, Zhao D, Yu XL, Ge M, Li RC, Jiang DL (2013) Small-sized circular genomes similar to genome of porcine circovirus 2. Genome Announc 1(2):e0009313CrossRefPubMed
11.
go back to reference Mankertz A, Mankertz J, Wolf K, Buhk HJ (1998) Identification of a protein essential for replication of porcine circovirus. J Gen Virol 79(2):381–384PubMed Mankertz A, Mankertz J, Wolf K, Buhk HJ (1998) Identification of a protein essential for replication of porcine circovirus. J Gen Virol 79(2):381–384PubMed
12.
go back to reference Nawagitgul P, Harms PA, Morozov I, Thacker BJ, Sorden SD, Lekcharoensuk C, Paul PS (2002) Modified indirect porcine circovirus (PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV. Clin Diagn Lab Immun 9(1):33–40 Nawagitgul P, Harms PA, Morozov I, Thacker BJ, Sorden SD, Lekcharoensuk C, Paul PS (2002) Modified indirect porcine circovirus (PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV. Clin Diagn Lab Immun 9(1):33–40
13.
go back to reference Nawagitgul P, Morozov I, Bolin SR, Harms PA, Sorden SD, Paul PS (2000) Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. J Gen Virol 81(9):2281–2287PubMed Nawagitgul P, Morozov I, Bolin SR, Harms PA, Sorden SD, Paul PS (2000) Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. J Gen Virol 81(9):2281–2287PubMed
14.
go back to reference Pensaert MB, Sanchez RE, Jr, Nauwynck HJ (2001) Transmission of porcine circovirus type 2 from the sow to the litter. In: Proceedings of the international conference on ssDNA viruses of plants, birds and primates, Saint Malo, France, p 84 Pensaert MB, Sanchez RE, Jr, Nauwynck HJ (2001) Transmission of porcine circovirus type 2 from the sow to the litter. In: Proceedings of the international conference on ssDNA viruses of plants, birds and primates, Saint Malo, France, p 84
15.
go back to reference Pesch S, Schmidt U, Ohlinger VF (2000) Proliferative necrotizing pneumonia (PNP) is a result of coinfection with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV-2). In: Proceedings of the 16th international pig veterinary society congress, Melbourne, Australia, p 581 Pesch S, Schmidt U, Ohlinger VF (2000) Proliferative necrotizing pneumonia (PNP) is a result of coinfection with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV-2). In: Proceedings of the 16th international pig veterinary society congress, Melbourne, Australia, p 581
16.
go back to reference Segalés J (2012) Porcine circovirus type 2 (PCV2) infections: clinical signs, pathology and laboratory diagnosis. Virus Res 164:10–19CrossRefPubMed Segalés J (2012) Porcine circovirus type 2 (PCV2) infections: clinical signs, pathology and laboratory diagnosis. Virus Res 164:10–19CrossRefPubMed
17.
go back to reference Segalés J, Domingo M, Latimer KS (1998) Porcine circovirus is present in cases of porcine dermatitis and nephropathy syndrome (PDNS). In: Proceedings of the 15th international pig veterinary society congress, Birmingham, England, p 215 Segalés J, Domingo M, Latimer KS (1998) Porcine circovirus is present in cases of porcine dermatitis and nephropathy syndrome (PDNS). In: Proceedings of the 15th international pig veterinary society congress, Birmingham, England, p 215
18.
go back to reference Segalés J, Olvera J, Grau-Roma L, Charreyre C, Nauwynck H, Larsen L, Dupont K, McCullough K, Ellis J, Krakowka S, Mankertz A, Fredholm M, Fossum C, Timmusk S, Stockhofe-Zurwieden N, Beattie V, Armstrong D, Grassland B, Baekbo P, Allan G (2008) PCV2 genotype definition and nomenclature. Vet Rec 162:867–868CrossRefPubMed Segalés J, Olvera J, Grau-Roma L, Charreyre C, Nauwynck H, Larsen L, Dupont K, McCullough K, Ellis J, Krakowka S, Mankertz A, Fredholm M, Fossum C, Timmusk S, Stockhofe-Zurwieden N, Beattie V, Armstrong D, Grassland B, Baekbo P, Allan G (2008) PCV2 genotype definition and nomenclature. Vet Rec 162:867–868CrossRefPubMed
19.
go back to reference Wen L, He K, Li B, Wang X, Guo R, Yu Z, Mao A, Ni Y, Zhang X, Zhou J, Lv L, Jiang J (2012) In vitro and in vivo isolation of a novel rearranged porcine circovirus type 2. J Virol 86(23):13120PubMedCentralCrossRefPubMed Wen L, He K, Li B, Wang X, Guo R, Yu Z, Mao A, Ni Y, Zhang X, Zhou J, Lv L, Jiang J (2012) In vitro and in vivo isolation of a novel rearranged porcine circovirus type 2. J Virol 86(23):13120PubMedCentralCrossRefPubMed
20.
go back to reference Wen L, He K, Ni Y, Zhang X, Li B, Wang X, Guo R, Yu Z, Mao A, Zhou J, Lv L, Jiang J (2012) Complete genome sequence of the rearranged porcine circovirus type 2. J Virol 86(10):5963PubMedCentralCrossRefPubMed Wen L, He K, Ni Y, Zhang X, Li B, Wang X, Guo R, Yu Z, Mao A, Zhou J, Lv L, Jiang J (2012) Complete genome sequence of the rearranged porcine circovirus type 2. J Virol 86(10):5963PubMedCentralCrossRefPubMed
21.
go back to reference Wen L, He K, Ni Y, Zhang X, Li B, Wang X, Guo R, Yu Z, Mao A, Zhou J, Lv L, Jiang J (2012) In vitro intragenomic rearrangement of porcine circovirus type 2. J Virol 86(16):8911PubMedCentralCrossRefPubMed Wen L, He K, Ni Y, Zhang X, Li B, Wang X, Guo R, Yu Z, Mao A, Zhou J, Lv L, Jiang J (2012) In vitro intragenomic rearrangement of porcine circovirus type 2. J Virol 86(16):8911PubMedCentralCrossRefPubMed
22.
go back to reference Wen L, He K, Xiao Q, Yu Z, Mao A, Ni Y, Zhang X, Li B, Wang X, Guo R, Zhou J, Lv L, Jiang J (2012) A novel porcine circovirus-like agent P1 is associated with wasting syndromes in pigs. PLoS One 7(8):e41565PubMedCentralCrossRefPubMed Wen L, He K, Xiao Q, Yu Z, Mao A, Ni Y, Zhang X, Li B, Wang X, Guo R, Zhou J, Lv L, Jiang J (2012) A novel porcine circovirus-like agent P1 is associated with wasting syndromes in pigs. PLoS One 7(8):e41565PubMedCentralCrossRefPubMed
23.
go back to reference Wen L, He K, Yang H, Ni Y, Zhang X, Guo R, Pan Q (2008) Complete nucleotide sequence of a novel porcine circovirus-like agent and its infectivity in vitro. Sci China C Life Sci 51(5):453–458CrossRefPubMed Wen L, He K, Yang H, Ni Y, Zhang X, Guo R, Pan Q (2008) Complete nucleotide sequence of a novel porcine circovirus-like agent and its infectivity in vitro. Sci China C Life Sci 51(5):453–458CrossRefPubMed
24.
go back to reference Wen L, He K, Yang H, Yu Z, Mao A, Zhong S, Ni Y, Zhang X, Li B, Wang X, Zhou J, Guo R, Lv L, Jiang J (2011) Prevalence of porcine circovirus-like agent P1 in Jiangsu, China. Virol J 8:543–547PubMedCentralCrossRefPubMed Wen L, He K, Yang H, Yu Z, Mao A, Zhong S, Ni Y, Zhang X, Li B, Wang X, Zhou J, Guo R, Lv L, Jiang J (2011) Prevalence of porcine circovirus-like agent P1 in Jiangsu, China. Virol J 8:543–547PubMedCentralCrossRefPubMed
25.
go back to reference Wen L, He K, Yu Z, Mao A, Ni Y, Zhang X, Guo RL, Li B, Wang X, Zhou J, Lv L (2012) Complete genome sequence of a novel porcine circovirus-like agent. J Virol 86(1):639PubMedCentralCrossRefPubMed Wen L, He K, Yu Z, Mao A, Ni Y, Zhang X, Guo RL, Li B, Wang X, Zhou J, Lv L (2012) Complete genome sequence of a novel porcine circovirus-like agent. J Virol 86(1):639PubMedCentralCrossRefPubMed
Metadata
Title
Recombination in truncated genome sequences of porcine circovirus type 2
Authors
Libin Wen
Fengzhi Wang
Kongwang He
Bin Li
Xiaomin Wang
Rongli Guo
Jianping Xie
Publication date
01-01-2015
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 1/2015
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-014-2242-9

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