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01-09-2011 | Brief Report

Vaccinia virus lacking A17 induces complex membrane structures composed of open membrane sheets

Authors: Petr Chlanda, Maria Alejandra Carbajal, Androniki Kolovou, Maho Hamasaki, Marek Cyrklaff, Gareth Griffiths, Jacomine Krijnse-Locker

Published in: Archives of Virology | Issue 9/2011

Abstract

The vaccinia virus (VACV) precursor membrane, the crescent, consists of an open membrane sheet and is formed by rupture of a cellular compartment. Here, we asked whether A17, a viral membrane protein, plays a role in membrane rupture. Without A17 synthesis, crescents are not formed, and instead, tubular and vesicular membranes accumulate (Rodriguez et al. in J Virol 69:4640–4648, 1). We used electron tomography (ET) to analyze whether the viral membranes lacking A17 consist of open membrane sheets. Tubular, vesicular and so far not described onion-shaped membranes, which consisted of open membrane sheets, were seen. Thus, the data show that membrane rupture occurs independently of the A17 protein.

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Title: Vaccinia virus lacking A17 induces complex membrane structures composed of open membrane sheets
Authors: Petr Chlanda
Maria Alejandra Carbajal
Androniki Kolovou
Maho Hamasaki
Marek Cyrklaff
Gareth Griffiths
Jacomine Krijnse-Locker
Publication date: 01-09-2011
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 9/2011
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-1012-1

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