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01-09-2011 | Brief Report

Design and evaluation of a recombinant multi-epitope-based ELISA for the serological surveillance of HEV infection in northern China

Authors: Gao Shenyang, Li Dandan, Fan Chen, Wu Shuliang, Zhou Tiezhong

Published in: Archives of Virology | Issue 9/2011

Abstract

In this study, seven recombinant epitope peptides from within the ORF2 protein of the local genotype 4 swine hepatitis E virus (HEV) DQ strain were designed and analyzed. Then, a new multi-epitope-based ELISA was established. In comparison with a commercial kit, this test exhibited good specificity and sensitivity for anti-HEV genotype 4. Subsequently, this test was applied for analyzing serum samples from either swine herds or human populations in northern China. The overall seroprevalence rate of anti-HEV IgG reached up to 40.4% for swine and 8.1% for humans. A statistical difference was observed for humans in rural and urban areas, with a higher prevalence for people living in rural than urban areas. Moreover, sequencing confirmed that all RNA-positive samples belonged to genotype 4.

Aye TT, Uchida T, Ma XZ, Iida F, Shikata T, Zhuang H, Win KM (1992) Complete nucleotide sequence of a hepatitis E virus isolated from the Xinjiang epidemic (1986–1988) of China. Nucleic Acids Res 20:3512PubMedCrossRef

Bouwknegt M, Engel B, Herremans MM, Widdowson MA, Worm HC, Koopmans MP, Frankena K, de Roda Husman AM, De Jong MC, Van Der Poel WH (2008) Bayesian estimation of hepatitis E virus seroprevalence for populations with different exposure levels to swine in the Netherlands. Epidemiol Infect 136:567–576PubMedCrossRef

Cai YS, Liu JH, Xu JX, Liang JH, Li ZQ, Zhang CH, Yun XX, Wang M (2010) Epidemiological characteristics of viral hepatitis E in Guangzhou. J Trop Med 1:90–91

Clemente-Casares P, Pina S, Buti M, Jardi R, Martin M, Bofill-Mas S, Girones R (2003) Hepatitis E virus epidemiology in industrialized countries. Emerg Infect Dis 9:448–454PubMed

Dong C, Dai X, Shao JS, Hu K, Meng JH (2007) Identification of genetic diversity of hepatitis E virus (HEV) and determination of the seroprevalence of HEV in eastern China. Arch Virol 152:739–746PubMedCrossRef

Emerson SU, Anderson D, Arankalle A, Meng XJ, Purdy M, Schlauder GG, Tsarev SA (2004) Hepevirus. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy, eighth report of the international committee on taxonomy of viruses. Elsevier Academic Press, London, pp 851–855

Guo H, Zhou EM, Sun ZF, Meng XJ, Halbur PG (2006) Identification of B-cell epitopes in the capsid protein of avian hepatitis E virus (avian HEV) that are common to human and swine HEVs or unique to avian HEV. J Gen Virol 87:217–223PubMedCrossRef

Khudyakov YE, Favorov MO, Jue DL, Hine TK, Fields HA (1994) Immunodominant antigenic regions in a structural protein of the hepatitis E virus. Virology 198:390–393PubMedCrossRef

Khudyakov YE, Khudyakova NS, Jue DL, Wells TW, Padhya N, Fields HA (1994) Comparative characterization of antigenic epitopes in the immunodominant region of the protein encoded by open reading frame 3 in Burmese and Mexican strains of hepatitis E virus. J Gen Virol 75:641–646PubMedCrossRef

10.

Khuroo MS, Teli MR, Skidmore S, Sofi MA, Khuroo MI (1981) Incidence and severity of viral hepatitis in pregnancy. Am J Med 70:252–255PubMedCrossRef

11.

Krawczynski K (1993) Hepatitis E. Hepatology 17:932–941PubMedCrossRef

12.

Dandan Li, Zhang XX, Zhao LP, Xiang WH (2008) Preparation and identification of monoclonal antibodies against ORF2-M1 protein of swine HEV. Prog Vet Med 5:27–31

13.

Meng XJ (2010) Hepatitis E virus: animal reservoirs and zoonotic risk. Vet Microbiol 140:256–365PubMedCrossRef

14.

Meng XJ (2010) Recent advances in Hepatitis E virus. J Viral Hepat 17:153–161PubMedCrossRef

15.

Riddell MichaelaA, Fan Li, Anderson DavidA (2000) Identification of immunodominant and conformational epitopes in the capsid protein of hepatitis E virus by using monoclonal antibodies. J Virol 74:8011–8017PubMedCrossRef

16.

Saha S, Raghava GPS (2004) BcePred: prediction of continuous B-cell epitopes in antigenic sequences using physico-chemical properties. In: Nicosia G, Cutello V, Bentley PJ, Timis J (eds) ICARIS. LNCS, vol 3239. Springer, Berlin, pp 197–204

17.

Schofield DJ, Glamann J, Emerson SU, Purcell RH (2000) Identification by phage display and characterization of two neutralizing chimpanzee monoclonal antibodies to the hepatitis E virus capsid protein. J Virol 74:5548–5555PubMedCrossRef

18.

Tei S, Kitajima N, Takahashi K, Mishiro S (2003) Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet 362(9381):371–373PubMedCrossRef

19.

Xiong JH, Guo QS, Ge SX, Gu Y, Chen YX, Miao J, Du HL, Shi WG, Zhang J, Xia NS (2008) The preliminary analysis of the recognition epitopes of anti-HEV monoclonal antibodies on HEV ORF2. Chin J Virology 2:83–87

20.

Yu M, Qu JJ, Guo W, Zhao LP, Xing WH (2006) The gene cloning and sequence analysis of the complete genome of swine Hepatitis E virus DQ strain. Chin J Pre Vet Med 28:507–509

21.

Zhang W, Yang S, Ren L, Shen Q, Cui L, Fan K, Huang F, Kang Y, Shan T, Wei J, Xiu H, Lou Y, Liu J, Yang Z, Zhu J, Hua X (2009) Hepatitis E virus infection in central China reveals no evidence of cross-species transmission between human and swine in this area. PLoS ONE 4(12):e8156. doi:10.1371/journal.pone.0008156

Title: Design and evaluation of a recombinant multi-epitope-based ELISA for the serological surveillance of HEV infection in northern China
Authors: Gao Shenyang
Li Dandan
Fan Chen
Wu Shuliang
Zhou Tiezhong
Publication date: 01-09-2011
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 9/2011
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-1007-y

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