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Archives of Virology
Published in: Archives of Virology 9/2010

01-09-2010 | Brief Report

Molecular epidemiology of porcine epidemic diarrhea virus in China

Authors: Jianfei Chen, Chengbao Wang, Hongyan Shi, Huaji Qiu, Shengwang Liu, Xiaojin Chen, Zhibang Zhang, Li Feng

Published in: Archives of Virology | Issue 9/2010

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Abstract

Since early 2006, porcine epidemic diarrhea virus (PEDV) has been reemerging in immunized swine herds. Open reading frame 3 (ORF3) is the only accessory gene in the PEDV genome. The entire ORF3 genes of 12 PEDV field strains and one vaccine strain were sequenced. The ORF3 genes of Chinese PEDV field strains (excluding CH/GSJIII/07) contain a single 672- or 675-nucleotide (nt) ORF, which encodes a 223- or 224-aa-long peptide. However, the CV777 vaccine strain and CH/GSJIII/07 contain a 276-nt ORF because of a 49-nt deletion at nt 245–293. The Chinese PEDV field strains and PEDV reference strains are divided into three groups based on the phylogenetic relationship of their ORF3 genes. Chinese PEDV field strains (excluding CH/GSJIII/07) have a close phylogenetic relationship to Korean strains and are genetically different from the PEDV vaccine strains. However, CH/GSJIII/07 has a close phylogenetic relationship to two vaccine strains, suggesting that it might have evolved from a live vaccine strain. Chinese PEDV field strains (excluding CH/GSJIII/07) can be differentiated from PEDV vaccine strains by a nested RT-PCR method.
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Metadata
Title
Molecular epidemiology of porcine epidemic diarrhea virus in China
Authors
Jianfei Chen
Chengbao Wang
Hongyan Shi
Huaji Qiu
Shengwang Liu
Xiaojin Chen
Zhibang Zhang
Li Feng
Publication date
01-09-2010
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 9/2010
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-010-0720-2

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