Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 9/2010

01-09-2010 | Original Article

Dengue-2-virus-interacting polypeptides involved in mosquito cell infection

Authors: Mandar S. Paingankar, Mangesh D. Gokhale, Dileep N. Deobagkar

Published in: Archives of Virology | Issue 9/2010

Login to get access

Abstract

For the design of effective antiviral strategies, understanding the fundamental steps of the virus life cycle, including virus–host interactions, is essential. We performed a virus overlay protein binding assay followed by proteomics for identification of proteins from membrane fractions of A7 (Aedes aegypti) cells, C6/36 (Aedes albopictus) cells and the midgut brush border membrane fraction of Ae. aegypti mosquito that bind to dengue-2 virus. Actin, ATP synthase β subunit, HSc 70, orisis, prohibitin, tubulin β chain, and vav-1 were identified as dengue-2-virus-binding proteins. Our results suggest that dengue-2 virus exploits an array of housekeeping proteins for its entry in mosquito cells.
Appendix
Available only for authorised users
Literature
1.
Arispe N, De Maio A (2000) ATP and ADP modulate a cation channel formed by Hsc70 in acid phospholipid membranes. J Biol Chem 275:30839–30843PubMedCrossRef
2.
Black WC, Bennett KE, Escalante-Gorrochotegui N, Barillas-Mury CV, Fernandez-Salas I, LourdesMunoz M, Farfan-Ale JA, Olson KE, Beaty BJ (2002) Flavivirus susceptibility in Aedes aegypti. Arch Med Res 33:379–388PubMedCrossRef
3.
Cao-Lormeau VM (2009) Dengue viruses binding proteins from Aedes aegypti and Aedes polynesiensis salivary glands. Virol J 6:35PubMedCrossRef
4.
Chavez-Salinas S, Ceballos-Olvera I, Reyes-del Valle J, Medina F, del Angel RM (2008) Heat shock effect upon dengue virus replication into U937 cells. Virus Res 138:111–118PubMedCrossRef
5.
Chee H, AbuBakar S (2004) Identification of a 48 kDa tubulin or tubulin-like C6/36 mosquito cells protein that binds dengue virus 2 using mass spectrometry. Biochem Biophys Res Commun 320:11–17PubMedCrossRef
6.
Chu JJH, Ng ML (2002) Trafficking mechanism of West Nile (Sarafend) virus structural proteins. J Med Virol 67:127–136PubMedCrossRef
7.
Fusaro G, Dasgupta P, Rastogi S, Joshi B, Chellappan S (2003) Prohibitin induces the transcriptional activity of p53 and is exported from the nucleus upon apoptotic signaling. J Biol Chem 278:47853–47861PubMedCrossRef
8.
9.
Hong SS, Ng ML (1987) Involvement of microtubules in Kunjin virus replication. Arch Virol 97:115–121PubMedCrossRef
10.
Houk EJ, Arcus YM, Hardy JL (1986) Isolation and characterization of brush border fragments from mesenterons. Arch Insect Biochem Physiol 3:135–146CrossRef
11.
Hung SL, Lee PL, Chen HW, Chen LK, Kao CL, King CC (1999) Analysis of the steps involved in Dengue virus entry into host cells. Virology 257:156–167PubMedCrossRef
12.
Liu WJ, Qi YM, Zhao KN, Liu YH, Liu XS, Frazer IH (2001) Association of bovine papillomavirus type 1 with microtubules. Virology 282:237–244PubMedCrossRef
13.
Lourenco-de-Oliveira R, Vazeille M, de Filippis AM, Failloux AB (2004) Ae. aegypti in Brazil: genetically differentiated populations with high susceptibility to dengue and yellow fever viruses. Trans R Soc Trop Med Hyg 98:43–54PubMedCrossRef
14.
Marone M, Mozzetti S, De Ritis D, Pierelli L, Scambia G (2001) Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample. Biol Proced Online 3:19–25PubMedCrossRef
15.
McClung JK, Jupe ER, Liu XT, Dell’Orco RT (1995) Prohibitin: potential role in senescence, development and tumor suppression. Exp Gerontol 30:99–124PubMedCrossRef
16.
Mendoza YM, Salas-Benito JS, Lanz-Mendoza H, Hernandez-Martinez S, del Angel RM (2002) A putative receptor for dengue virus in mosquito tissues: localization of a 45-kDa glycoprotein. Am J Trop Med Hyg 67:76–84
17.
Mercado-Curiel RF, WC Black IV, Lourdes Muñoz M (2008) A dengue receptor as possible genetic marker of vector competence in Aedes aegypti. BMC Microbiol 8:118PubMedCrossRef
18.
Mercado-Curiel RF, Esquinca-Avilés HA, Tovar R, Díaz-Badillo A, Camacho-Nuez M, de Muñoz ML (2006) The four serotypes of dengue recognize the same putative receptors in Aedes aegypti midgut and Ae. albopictus cells. BMC Microbiol 6:85PubMedCrossRef
19.
Mishra S, Moulik S, Murphy LJ (2007) Prohibitin binds to C3 and enhances complement activation. Mol Immunol 44:1897–1902PubMedCrossRef
20.
Mishra S, Murphy LC, Murphy LJ (2006) The Prohibitins: emerging roles in diverse functions. J Cell Mol Med 10:353–363PubMedCrossRef
21.
Modis Y, Ogata S, Clements D, Harrison SC (2003) A ligand binding pocket in Dengue virus envelop glycoprotein. Proc Natl Acad Sci USA 100:6986–6991PubMedCrossRef
22.
Moyer SA, Baker SC, Lessard JL (1986) Tubulin: a factor necessary for the synthesis of both Sendai virus and vesicular stomatitis virus RNAs. Proc Natl Acad Sci USA 83:5405–5409PubMedCrossRef
23.
Ng ML, Hong SS (1989) Flavivirus infection: essential ultrastructural changes and association of Kunjin virus NS3 protein with microtubules. Arch Virol 106:103–120PubMedCrossRef
24.
Padwad YS, Mishra KP, Jain M, Chanda S, Karan D, Ganju L (2009) RNA interference mediated silencing of Hsp60 gene in human monocytic myeloma cell line U937 revealed decreased dengue virus multiplication. Immunobiology 214:422–429PubMedCrossRef
25.
Ren J, Ding T, Zhang W, Song J, Ma W (2007) Does Japanese encephalitis virus share the same cellular receptor with other mosquito-borne flaviviruses on the C6/36 mosquito cells? Virol J 4:83PubMedCrossRef
26.
Rey FA (2003) Dengue virus envelope glycoprotein structure: new insight into its interactions during viral entry. Proc Natl Acad Sci USA 100:6899–6901PubMedCrossRef
27.
Reyes Del Valle J, Chávez-Salinas S, Medina F, del Angel RM (2005) Heat shock protein 90 and heat shock protein 70 are components of dengue virus receptor complex in human cells. J Virol 79:4557–4567PubMedCrossRef
28.
Salas-Benito J, Valle JR-D, Salas-Benito M, Ceballos-Olvera I, Mosso C, Del Angel RM (2007) Evidence that the 45-kD glycoprotein, part of a putative dengue virus receptor complex in the mosquito cell line C6/36, is a heat-shock-related protein. Am J Trop Med Hyg 77:283–290PubMed
29.
Salas-Benito JS, del Angel RM (1997) Identification of two surface proteins from C6/36 cells that bind dengue type 4 virus. J Virol 71:7246–7252PubMed
30.
Sharma A, Qadri A (2004) Vi polysaccharise of Salmonella typhi target the prohibitin family of molecules in intestinal epithelial cells and suppress early inflammatory responses. Proc Natl Acad Sci USA 101:17492–17497PubMedCrossRef
31.
Smith GA, Enquist LW (2002) Break ins and break outs: viral interactions with the cytoskeleton of mammalian cells. Annu Rev Cell Dev Biol 18:135–161PubMedCrossRef
32.
Suomalainen M, Nakano MY, Boucke K, Keller S, Stidwill RP, Greber UF (1999) Microtubule-dependent minus and plus end-directed motilities are competing processes for nuclear targeting of adenovirus. J Cell Biol 144:657–672PubMedCrossRef
33.
Talavera D, Castillo AM, Dominguez MC, Gutierrez AE, Meza I (2004) IL8 release, tight junction and cytoskeleton dynamic reorganization conducive to permeability increase are induced by dengue virus infection of microvascular endothelial monolayers. J Gen Virol 85:1801–1813PubMedCrossRef
34.
Upanan S, Kuadkitkan A, Smith DR (2008) Identification of dengue virus binding proteins using affinity chromatography. J Virol Methods 151:325–328PubMedCrossRef
35.
Villas-Bôas CSA, Conceição TM, Ramírez J, Santoro ABM, Da Poian AT, Montero-Lomelí M (2009) Dengue virus-induced regulation of the host cell translational machinery. Braz J Med Biol Res 42(11):1020–1026PubMedCrossRef
36.
Wang S, Zhang B, Faller DV (1999) Rb and prohibitin target distinct regions of E2F1 for repression and respond to different upstream signals. Mol Cell Biol 19:7447–7460PubMed
37.
Waterboer T, Rahaus M, Wolff MH (2002) Varicellazoster virus (VZV) mediates a delayed host shutoff independent of open reading frame (ORF) 17 expression. Virus Genes 24:49–56PubMedCrossRef
38.
Wolfersberger M, Liithy P, Maurer A, Parenti P, Sacchi VF, Giordana B, Hanozel GM (1987) Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the cabbage butterfly (Pieris brassicae). Comp Biochem Physiol 86:301–308CrossRef
39.
Xu A, Bellamy AR, Taylor JA (2000) Immobilization of the early secretory pathway by a virus glycoprotein that binds to microtubules. EMBO J 19:6465–6474PubMedCrossRef
Metadata
Title
Dengue-2-virus-interacting polypeptides involved in mosquito cell infection
Authors
Mandar S. Paingankar
Mangesh D. Gokhale
Dileep N. Deobagkar
Publication date
01-09-2010
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 9/2010
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-010-0728-7

Other articles of this Issue 9/2010

Archives of Virology 9/2010 Go to the issue

Brief Report

Rugose wood-associated viruses do not appear to be involved in Shiraz (Syrah) decline in South Africa

Annotated Sequence Record

Complete nucleotide sequence of Sida golden mosaic Florida virus and phylogenetic relationships with other begomoviruses infecting malvaceous weeds in the Caribbean

Brief Report

Status of foot-and-mouth disease in Pakistan

Annotated Sequence Record

The complete genome sequence and genome structure of frangipani mosaic virus

Brief Report

First molecular detection of Aichi virus in sewage and shellfish samples in the Monastir region of Tunisia

Original Article

Pathobiological characterization of low-pathogenicity H5 avian influenza viruses of diverse origins in chickens, ducks and turkeys
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits