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Archives of Virology
Published in: Archives of Virology 7/2010

01-07-2010 | Original Article

Biological and molecular characterization of Capsicum chlorosis virus infecting chilli and tomato in India

Authors: S. R. Kunkalikar, P. Sudarsana, P. Rajagopalan, Usha B. Zehr, K. S. Ravi

Published in: Archives of Virology | Issue 7/2010

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Abstract

Two isolates of Capsicum chlorosis virus (CaCV, genus Tospovirus) from tomato (CaCV-To-Ind) and chilli (CaCV-Ch-Pan), collected from Haryana and Uttar Pradesh states of northern India respectively, were compared. A comparison of the amino acid sequences of their N genes revealed more than 96% identity, confirming that the virus isolates in India have a high degree of sequence conservation and are closely related to Australian isolates. Analysis of the host range of CaCV revealed no biological difference between the isolates, but they differed from CaCV-Australia. The nucleotide sequences of S, M and L RNA of CaCV-Ch-Pan were determined. The S RNA contains 3,105 nucleotides (nt), with NSs and N genes of 1,320 and 828 nt, respectively. The M RNA consists of 4,821 nt, with an NSm gene of 927 nt and a Gn/Gc gene of 3,366 nt. The intergenic regions of S and M RNA contain 824 and 425 nt, respectively. The L RNA consists of 8,912 nt, with an RNA-dependent RNA polymerase gene of 8,634 nt.
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Metadata
Title
Biological and molecular characterization of Capsicum chlorosis virus infecting chilli and tomato in India
Authors
S. R. Kunkalikar
P. Sudarsana
P. Rajagopalan
Usha B. Zehr
K. S. Ravi
Publication date
01-07-2010
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 7/2010
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-010-0681-5

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