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01-09-2009 | Original Article

Complete genome analysis of a rabies virus isolate from Brazilian wild fox

Authors: Nobuyuki Mochizuki, Yuki Kobayashi, Go Sato, Takuya Itou, Alberio Antonio Barros Gomes, Fumio Honma Ito, Takeo Sakai

Published in: Archives of Virology | Issue 9/2009

Abstract

The complete genome sequence of wild-type rabies virus (RABV) isolated from a wild Brazilian hoary fox (Dusicyon sp.), the BR-Pfx1 isolate, was determined and compared with fixed RABV strains. The genome structure and organization of the BR-Pfx1 isolate were composed of 11,924 nt and included the five standard genes of rhabdoviruses. Sequences of mRNA start and stop signals for transcription were highly conserved among all structural protein genes of the BR-Pfx1 isolate. All amino acid residues in the glycoprotein (G) gene associated with pathogenicity were retained in the BR-Pfx1 isolate, while unique amino acid substitutions were found in antigenic region I of the nucleoprotein gene and III of G. These results suggest that although the standard genome structure and organization of the RABV isolate are common between the BR-Pfx1 isolate and fixed RABV strains, the unique amino acid substitutions in functional sites of the BR-Pfx1 isolate may result in different biological characteristics from fixed RABV strains.

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Title: Complete genome analysis of a rabies virus isolate from Brazilian wild fox
Authors: Nobuyuki Mochizuki
Yuki Kobayashi
Go Sato
Takuya Itou
Alberio Antonio Barros Gomes
Fumio Honma Ito
Takeo Sakai
Publication date: 01-09-2009
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 9/2009
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-009-0475-9

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