Top

Published in:

01-07-2008 | Brief Report

Construction of an infectious full-length cDNA clone of potato virus M

Authors: S. Flatken, V. Ungewickell, W. Menzel, E. Maiss

Published in: Archives of Virology | Issue 7/2008

Abstract

An infectious full-length cDNA clone of potato virus M (PVM) was produced. Total RNA was extracted from PVM-infected Nicotiana hesperis plants and used for cDNA synthesis. Subsequent RT-PCR produced two DNA fragments of about 5.5 and 3.2 kbp, which were ligated downstream of an enhanced 35S cauliflower mosaic virus promoter. After cloning of the enhanced 35S promoter with the PVM sequence into a modified pBIN19 plasmid and electroporation of Agrobacterium tumefaciens, the agroinoculated PVM full-length clone (pPVM-flc) led to systemic PVM infections in different host plants, causing symptoms indistinguishable from those caused by wild-type PVM.

Aritua V, Barg E, Adipala E, Vetten HJ (2007) Sequence analysis of the entire RNA genome of a sweet potato chlorotic fleck virus isolate reveals that it belongs to a distinct carlavirus species. Arch Virol 152:813–818PubMedCrossRef

Berger PH, Hunt AG, Domier LL, Hellmann GM, Stram Y, Thornbury DW, Pirone TP (1989) Expression in transgenic plants of a viral gene product that mediates insect transmission of potyviruses. Proc Natl Acad Sci USA 86:8402–8406PubMedCrossRef

Carrington JC, Kasschau KD, Mahajan SK, Schaad MC (1996) Cell-to cell and long-distance transport of viruses in plants. Plant Cell 8:1669–1681PubMedCrossRef

Cavileer TD, Halpern BT, Lawrence DM, Podleckis EV, Martin RR, Hillman BI (1994) Nucleotide sequence of the carlavirus associated with blueberry scorch and similar diseases. J Gen Virol 75:711–720PubMedCrossRef

Choi SA, Ryu KH (2003) The complete nucleotide sequence of the genome RNA of Lily symptomless virus and its comparison with that of other carlaviruses. Arch Virol 148:1943–1955PubMedCrossRef

English JJ, Davenport GF, Elmayan T, Vaucheret H, Baulcombe DC (1997) Requirement of sense transcription for homology-dependent virus resistance and trans-inactivation. Plant J 12:597–603CrossRef

Fuji S, Yamamoto H, Inoue M, Yamashita K, Fukui Y, Furuya H, Naito H (2002) Complete nucleotide sequence of the genomic RNA of Aconitum latent virus (genus Carlavirus) isolated from Delphinium sp. Arch Virol 147:865–870PubMedCrossRef

Gramstat A, Courtpzanis A, Rhode W (1990) The 12 kDa protein of potato virus M displays properties of a nucleic acid binding protein. FEBS Lett 276(34):38

Hataya T, Arimoto R, Suda N, Uyeda I (2001) Molecular characterization of hop mosaic virus: its serological and molecular relationship to hop latent virus. Arch Virol 146:1935–1948PubMedCrossRef

10.

Hataya T, Uchino K, Arimoto R, Suda N, Sano T, Shikata E, Uyeda I (2000) Molecular characterization of Hop latent virus and phylogenetic relationships among viruses closely related to carlaviruses. Arch Virol 145:2503–2524PubMedCrossRef

11.

Heitefuss R, König K, Obst A, Reschke M (1993) Pflanzenkrankheiten und Schädlinge im Ackerbau. 3.Auflage Verlagsunion Agrar, Frankfurt am Main

12.

Hoekema A, Hirsch PR, Hooykaas PJJ, Schilpoort RA (1983) A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303:179–180CrossRef

13.

Howard AR, Heppler ML, Ju HJ, Krishnamurthy K, Payton ME, Verchot-Lubicz J (2004) Potato virus X TGBp1 induces plasmodesmata gating and moves between cells in several host species whereas CP moves only in N. benthamiana leaves. Virology 328:185–197PubMedCrossRef

14.

Huisman MJ, Linthorst HJM, Bol JF, Cornelissen BJC (1998) The complete nucleotide sequence of Potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. J Gen Virol 69:1789–1798CrossRef

15.

Ju HJ, Samuels TD, Wang YS, Blancaflor E, Payton M, Mitra R, Krishnamurthy K, Nelson RS, Verchot-Lubicz J (2005) The potato virus X TGBp2 movement protein associates with endoplasmic reticulum-derived vesicles during virus infection. Plant Physiol 138:1877–1895PubMedCrossRef

16.

Koonin EV, Dolja VV (1993) Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol 28:375–430PubMedCrossRef

17.

Krishnamurthy K, Mitra R, Payton ME, Verchot-Lubicz J (2002) Cell-to-cell movement of the PVX 12K, 8K, or coat proteins may depend on the host, leaf developmental stage, and the PVX 25 K protein. Virology 300:269–281PubMedCrossRef

18.

Lawrence DM, Hillman I (1994) Synthesis of infectious transcripts of blueberry scorch carlavirus in vitro. J Gen Virol 75:2509–2512PubMedCrossRef

19.

Lee BY, Min BE, Ha JH, Lee MY, Paek KH, Ryu KH (2006) Genome structure and complete sequence of genomic RNA of Daphne virus S. Arch Virol 151:193–200PubMedCrossRef

20.

Lin NS, Hsu YT, Hsu HT (1990) Immunological detection of plant viruses and a mycoplasmalike organism by direct tissue blotting on nitrocellulose membranes. Phytopathology 80:824–828CrossRef

21.

Menzel W, Zahn V, Maiss E (2003) Multiplex RT-PCR-ELISA compared with bioassay for the detection of four apple viruses. J Virol Methods 110:153–157PubMedCrossRef

22.

Milne RG, Lesemann DE (1984) Immunosorbent electron microscopy in plant virus studies. Methods Virol 8:85–101

23.

Morozov S, Solovyev AG (2003) Triple gene block: modular design of a multifunctional machine for plant virus movement. J Gen Virol 84:1351–1366PubMedCrossRef

24.

Morris TJ, Dodds JA (1979) Isolation of double-stranded RNA from virus infected plants. Phytopathology 69:854–858CrossRef

25.

Naylor M, Reeves J, Cooper JI, Edwards ML, Wang H (2005) Construction and properties of a gene-silencing vector based on Poplar mosaic virus (genus Carlavirus). J Virol Methods 124:27–36PubMedCrossRef

26.

Ohkawa A, Yamada M, Sayama H, Sugiyama N, Okuda S, Natsuaki T (2007) Complete nucleotide sequence of a Japanese isolate of Chrysanthemum virus B (genus Carlavirus). Arch Virol 152:2253–2258PubMedCrossRef

27.

Pluthero FG (1993) Rapid purification of high-activity Taq DNA polymerase. Nucleic Acids Res 21:4850–4851PubMedCrossRef

28.

Proll E, Leiser RM, Ostermann WD, Spaar D (1981) Einige physikochemische Eigenschaften des Kartoffel-virus M. Potato Res 24:1CrossRef

29.

Rozanov MN, Koonin EV, Gorbalenya AE (1992) Conservation of the putative methyltransferase domain: a hallmark of the “Sindbis-like” supergroup of positive-strand RNA viruses. J Gen Virol 73:2129–2134PubMedCrossRef

30.

Rupasov VV, Morozov SY, Kanyuka KV, Zavriev SK (1989) Partial nucleotide sequence of Potato virus M RNA shows similarities to potexviruses in gene arrangement and the encoded amino acid sequences. J Gen Virol 70:1861–1869PubMedCrossRef

31.

Schägger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368–379PubMedCrossRef

32.

Schultz E, Folsom D (1923) Transmission, variation, and control of certain degeneration diseases of Irish potatoes. J Agric Res 25:43–118

33.

Scott SW, Zimmerman MT (2008) The complete sequence of ligustrum necrotic ringspot virus, a novel carlavirus. Arch Virol 153:393–396PubMedCrossRef

34.

Smith CM, Campbell MM (2004) Complete nucleotide sequence of the genomic RNA of Poplar mosaic virus (genus Carlavirus). Arch Virol 149:1831–1841PubMedCrossRef

35.

Song SI, Choi JN, Song JT, Ahn JH, Lee JS, Kim M, Cheong JJ, Choi YD (2002) Complete genome sequence of garlic latent virus, a member of the carlavirus family. Mol Cells 14:205–213PubMed

36.

Spiegel S, Zeidan M, Sobolev I, Beckelman Y, Holdengreber V, Tam Y, Bar Joseph M, Lipsker Z, Gera A (2007) The complete nucleotide sequence of Passiflora latent virus and its phylogenetic relationship to other carlaviruses. Arch Virol 152:181–189PubMedCrossRef

37.

Stephan D, Maiss E (2006) Biological properties of Beet mild yellowing virus derived from a full-length cDNA clone. J Gen Virol 87:445–449PubMedCrossRef

38.

Stevenson WR, Loria R, Franc GD, Weingartner DP (2001) Compendium of potato diseases, 2nd edn. The American Phytopathological Society, St. Paul

39.

Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882PubMedCrossRef

40.

Töpfer R, Matzeit V, Gronenborn B, Shell J, Steinbiss HH (1987) A set of plant expressing vectors for transcriptional and translational fusions. Nucleic Acids Res 15:5890PubMedCrossRef

41.

Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354PubMedCrossRef

42.

Wetter C, Milne RG (1981) Carlaviruses. In: Kurstak E (ed) Handbook of plant virus infections and comparative diagnosis. Elsevier, Amsterdam, p 695

43.

Zavriev SK, Kanyuka KV, Levay KE (1991) The genome organization of potato virus M RNA. J Gen Virol 72:9–14PubMedCrossRef

44.

Zheng HY, Chen J, Adams MJ, Chen JP (2006) Complete nucleotide sequence and affinities of the genomic RNA of Narcissus common latent virus (genus Carlavirus). Arch Virol 151:1667–1672PubMedCrossRef

Title: Construction of an infectious full-length cDNA clone of potato virus M
Authors: S. Flatken
V. Ungewickell
W. Menzel
E. Maiss
Publication date: 01-07-2008
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 7/2008
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-008-0127-5

ACC 2024 Congress Coverage

Heart Failure Video

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Construction of an infectious full-length cDNA clone of potato virus M

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 7/2008

Genomic analysis of Newcastle disease virus strain NA-1 isolated from geese in China

Analysis of some phenotypic traits of feces-borne temperate lambdoid bacteriophages from different immunity groups: a high incidence of cor+, FhuA-dependent phages

Early transcriptional response in the jejunum of germ-free piglets after oral infection with virulent rotavirus

Dynamic analysis of genetic diversity of gag and env regions of HIV-1 CRF07_BC recombinant in intravenous drug users in Xinjiang Uvghur Autonomous Region, China

Cell-permeable artificial zinc-finger proteins as potent antiviral drugs for human papillomaviruses

High-mobility group protein A1 binds herpes simplex virus gene regulatory sequences and affects their expression

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page