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01-12-2008 | Review

Tissue engineering and the intervertebral disc: the challenges

Authors: Rita Kandel, Sally Roberts, Jill P. G. Urban

Published in: European Spine Journal | Special Issue 4/2008

Abstract

Disc degeneration is a common disorder. Although the back pain that can develop in association with this is rarely life-threatening, the annual cost in terms of morbidity, lost productivity, medical expenses and workers’ compensation benefits is significant. Surgical intervention as practised currently is directed towards removing the damaged or altered tissue. Development of new treatment modalities is critical as there is a growing consensus that the strategies used currently for symptomatic degenerative disc disease may not be effective. Accordingly, there is a need to develop an entirely new way to treat this disorder; regenerative medicine and tissue engineering approaches appear particularly promising in this regard. This paper reviews some of the challenges that currently are limiting the clinical application of this approach to the treatment of disc degeneration.

Adams MA, Roughley PJ (2006) What is intervertebral disc degeneration, and what causes it? Spine 31(18):2151–2161PubMedCrossRef

Agrawal A, Guttapalli A, Narayan S, Albert TJ, Shapiro IM, Risbud MV (2007) Normoxic stabilization of HIF-1alpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk. Am J Physiol Cell Physiol 293(2):C621–C631PubMedCrossRef

Aguiar DJ, Johnson SL, Oegema TR (1999) Notochordal cells interact with nucleus pulposus cells: regulation of proteoglycan synthesis. Exp Cell Res 246(1):129–137PubMedCrossRef

Ahmed N, Stanford WL, Kandel RA (2007) Mesenchymal stem and progenitor cells for cartilage repair. Skeletal Radiol 36(10):909–912PubMedCrossRef

Alini M, Eisenstein SM, Ito K, Little C, Kettler AA, Masuda K et al (2008) Are animal models useful for studying human disc disorders/degeneration? Eur Spine J 17(1):2–19PubMedCrossRef

Anderson DG, Risbud MV, Shapiro IM, Vaccaro AR, Albert TJ (2005) Cell-based therapy for disc repair. Spine J 5(6 Suppl):297S–303SPubMedCrossRef

Bajada S, Harrison PE, Ashton BA, Cassar-Pullicino VN, Ashammakhi N, Richardson JB (2007) Successful treatment of refractory tibial nonunion using calcium sulphate and bone marrow stromal cell implantation. J Bone Joint Surg Br 89(10):1382–1386PubMedCrossRef

Bartels EM, Fairbank JC, Winlove CP, Urban JP (1998) Oxygen and lactate concentrations measured in vivo in the intervertebral discs of patients with scoliosis and back pain. Spine 23(1):1–7PubMedCrossRef

Bashur CA, Dahlgren LA, Goldstein AS (2006) Effect of fiber diameter and orientation on fibroblast morphology and proliferation on electrospun poly(d, l-lactic-co-glycolic acid) meshes. Biomaterials 27(33):5681–5688PubMedCrossRef

10.

Battie MC, Videman T (2006) Lumbar disc degeneration: epidemiology and genetics. J Bone Joint Surg Am 88(Suppl 2):3–9PubMedCrossRef

11.

Benneker LM, Heini PF, Alini M, Anderson SE, Ito K (2005) 2004 Young Investigator Award Winner: vertebral endplate marrow contact channel occlusions and intervertebral disc degeneration. Spine 30(2):167–173PubMedCrossRef

12.

Bibby SR, Fairbank JC, Urban MR, Urban JP (2002) Cell viability in scoliotic discs in relation to disc deformity and nutrient levels. Spine 27(20):2220–2228PubMedCrossRef

13.

Bibby SR, Jones DA, Ripley RM, Urban JP (2005) Metabolism of the intervertebral disc: effects of low levels of oxygen, glucose, and pH on rates of energy metabolism of bovine nucleus pulposus cells. Spine 30(5):487–496PubMedCrossRef

14.

Bibby SR, Urban JP (2004) Effect of nutrient deprivation on the viability of intervertebral disc cells. Eur Spine J 13(8):695–701PubMedCrossRef

15.

Bieback K, Kern S, Kocaomer A, Ferlik K, Bugert P (2008) Comparing mesenchymal stromal cells from different human tissues: bone marrow, adipose tissue and umbilical cord blood. Biomed Mater Eng 18(1 Suppl):S71–S76PubMed

16.

Boden SD (2002) Overview of the biology of lumbar spine fusion and principles for selecting a bone graft substitute. Spine 27(16 Suppl 1):S26–S31PubMedCrossRef

17.

Bogduk N (1997) The inter-body joints and the intervertebral discs. In: Bogduk N (ed) Clinical anatomy of the lumbar spine and sarcum. Churchill Livingstone, New York, pp 13–31

18.

Boos N, Weissbach S, Rohrbach H, Weiler C, Spratt KF, Nerlich AG (2002) Classification of age-related changes in lumbar intervertebral discs: 2002 Volvo Award in basic science. Spine 27(23):2631–2644PubMedCrossRef

19.

Brisby H, Tao H, Ma DD, Diwan AD (2004) Cell therapy for disc degeneration—potentials and pitfalls. Orthop Clin North Am 35(1):85–93PubMedCrossRef

20.

Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O, Peterson L (1994) Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 331(14):889–895PubMedCrossRef

21.

Chang G, Kim HJ, Kaplan D, Vunjak-Novakovic G, Kandel RA (2007) Porous silk scaffolds can be used for tissue engineering annulus fibrosus. Eur Spine J 16(11):1848–1857PubMedCrossRef

22.

Chang NK, Jeong YY, Park JS, Jeong HS, Jang S, Jang MJ et al (2008) Tracking of neural stem cells in rats with intracerebral hemorrhage by the use of 3T MRI. Korean J Radiol 9(3):196–204PubMed

23.

Chen J, Yan W, Setton LA (2006) Molecular phenotypes of notochordal cells purified from immature nucleus pulposus. Eur Spine J 15(Suppl 3):S303–S311PubMedCrossRef

24.

Chen M, Patra PK, Warner SB, Bhowmick S (2007) Role of fiber diameter in adhesion and proliferation of NIH 3T3 fibroblast on electrospun polycaprolactone scaffolds. Tissue Eng 13(3):579–587PubMedCrossRef

25.

Elliott DM, Yerramalli CS, Beckstein JC, Boxberger JI, Johannessen W, Vresilovic EJ (2008) The effect of relative needle diameter in puncture and sham injection animal models of degeneration. Spine 33(6):588–596PubMed

26.

Frick SL, Hanley EN Jr, Meyer RA Jr, Ramp WK, Chapman TM (1994) Lumbar intervertebral disc transfer. A canine study. Spine 19(16):1826–1834PubMedCrossRef

27.

Frymoyer JW, Cats-Baril WL (1991) An overview of the incidences and costs of low back pain. Orthop Clin North Am 22(2):263–271PubMed

28.

Garvin PJ, Jennings RB, Smith L, Gesler RM (1965) Chymopapain: a pharmacologic and toxicologic evaluation in experimental animals. Clin Orthop Relat Res 41:204–223PubMedCrossRef

29.

Gold GE, Beaulieu CF (2001) Future of MR imaging of articular cartilage. Semin Musculoskelet Radiol 5(4):313–327PubMedCrossRef

30.

Gruber HE, Hanley EN Jr (1998) Analysis of aging and degeneration of the human intervertebral disc. Comparison of surgical specimens with normal controls. Spine 23(7):751–757PubMedCrossRef

31.

Gruber HE, Ingram JA, Davis DE, Hanley EN Jr (2008) Increased cell senescence is associated with decreased cell proliferation in vivo in the degenerating human annulus. Spine J (in press)

32.

Gruber HE, Johnson TL, Leslie K, Ingram JA, Martin D, Hoelscher G et al (2002) Autologous intervertebral disc cell implantation: a model using Psammomys obesus, the sand rat. Spine 27(15):1626–1633PubMedCrossRef

33.

Gruber HE, Leslie K, Ingram J, Norton HJ, Hanley EN (2004) Cell-based tissue engineering for the intervertebral disc: in vitro studies of human disc cell gene expression and matrix production within selected cell carriers. Spine J 4(1):44–55PubMedCrossRef

34.

Grunhagen T, Wilde G, Soukane DM, Shirazi-Adl SA, Urban JP (2006) Nutrient supply and intervertebral disc metabolism. J Bone Joint Surg Am 88(Suppl 2):30–35PubMedCrossRef

35.

Halloran DO, Grad S, Stoddart M, Dockery P, Alini M, Pandit AS (2008) An injectable cross-linked scaffold for nucleus pulposus regeneration. Biomaterials 29(4):438–447PubMedCrossRef

36.

Hamilton DJ, Seguin CA, Wang J, Pilliar RM, Kandel RA (2006) Formation of a nucleus pulposus-cartilage endplate construct in vitro. Biomaterials 27(3):397–405PubMedCrossRef

37.

Harrison PE, Ashton IK, Johnson WE, Turner SL, Richardson JB, Ashton BA (2000) The in vitro growth of human chondrocytes. Cell Tissue Bank 1(4):255–260PubMedCrossRef

38.

Haufe SM, Mork AR (2006) Intradiscal injection of hematopoietic stem cells in an attempt to rejuvenate the intervertebral discs. Stem Cells Dev 15(1):136–137PubMedCrossRef

39.

Hiyama A, Mochida J, Iwashina T, Omi H, Watanabe T, Serigano K et al (2008) Transplantation of mesenchymal stem cells in a canine disc degeneration model. J Orthop Res 26(5):589–600PubMedCrossRef

40.

Holm S, Maroudas A, Urban JP, Selstam G, Nachemson A (1981) Nutrition of the intervertebral disc: solute transport and metabolism. Connect Tissue Res 8(2):101–119PubMedCrossRef

41.

Horner HA, Roberts S, Bielby RC, Menage J, Evans H, Urban JP (2002) Cells from different regions of the intervertebral disc: effect of culture system on matrix expression and cell phenotype. Spine 27(10):1018–1028PubMedCrossRef

42.

Horner HA, Urban JP (2001) 2001 Volvo Award Winner in Basic Science Studies: effect of nutrient supply on the viability of cells from the nucleus pulposus of the intervertebral disc. Spine 26(23):2543–2549PubMedCrossRef

43.

Hukins DW (1988) Disc structure and function. In: Ghosh P (ed) Biology of intervertebral disc. CRC Press, Boca Raton, pp 2–37

44.

Hunter CJ, Matyas JR, Duncan NA (2003) The notochordal cell in the nucleus pulposus: a review in the context of tissue engineering. Tissue Eng 9(4):667–677PubMedCrossRef

45.

Ikada Y (2006) Challenges in tissue engineering. J R Soc Interface 3(10):589–601PubMedCrossRef

46.

Iron KS, Manuel DG, Williams J (2004) Using a linked data set to determine the factors associated with utilization and costs of family physician services in Ontario: effects of self-reported chronic conditions. Chronic Dis Can 24(4):124–132

47.

Ishihara H, Urban JP (1999) Effects of low oxygen concentrations and metabolic inhibitors on proteoglycan and protein synthesis rates in the intervertebral disc. J Orthop Res 17(6):829–835PubMedCrossRef

48.

Javedan SP, Dickman CA (1999) Cause of adjacent-segment disease after spinal fusion. Lancet 354(9178):530–531PubMedCrossRef

49.

Johannessen W, Auerbach JD, Wheaton AJ, Kurji A, Borthakur A, Reddy R et al (2006) Assessment of human disc degeneration and proteoglycan content using T1rho-weighted magnetic resonance imaging. Spine 31(11):1253–1257PubMedCrossRef

50.

Kalichman L, Hunter DJ (2007) Lumbar facet joint osteoarthritis: a review. Semin Arthritis Rheum 37(2):69–80PubMedCrossRef

51.

Kandel RA, Grynpas M, Pilliar R, Lee J, Wang J, Waldman S et al (2006) Repair of osteochondral defects with biphasic cartilage-calcium polyphosphate constructs in a sheep model. Biomaterials 27(22):4120–4131PubMedCrossRef

52.

Kandel RA, Hamilton D, Seguin C, Li SQ, Arana C, Pilliar R (2007) An in vitro tissue model to study the effect of age on nucleus pulposus cells. Eur Spine J 16(12):2166–2173PubMedCrossRef

53.

Kim PK, Branch CL Jr (2006) The lumbar degenerative disc: confusion, mechanics, management. Clin Neurosurg 53:18–25PubMed

54.

Korecki CL, Costi JJ, Iatridis JC (2008) Needle puncture injury affects intervertebral disc mechanics and biology in an organ culture model. Spine 33(3):235–241PubMedCrossRef

55.

Lange C, Cakiroglu F, Spiess AN, Cappallo-Obermann H, Dierlamm J, Zander AR (2007) Accelerated and safe expansion of human mesenchymal stromal cells in animal serum-free medium for transplantation and regenerative medicine. J Cell Physiol 213(1):18–26PubMedCrossRef

56.

Le Maitre CL, Hoyland JA, Freemont AJ (2007) Catabolic cytokine expression in degenerate and herniated human intervertebral discs: IL-1beta and TNFalpha expression profile. Arthritis Res Ther 9(4):R77PubMedCrossRef

57.

Le VC, Kim SW, Tateno K, Sieber AN, Kostuik JP, Leong KW (2006) Interaction of human mesenchymal stem cells with disc cells: changes in extracellular matrix biosynthesis. Spine 31(18):2036–2042CrossRef

58.

Lee CR, Sakai D, Nakai T, Toyama K, Mochida J, Alini M et al (2007) A phenotypic comparison of intervertebral disc and articular cartilage cells in the rat. Eur Spine J 16(12):2174–2185PubMedCrossRef

59.

Lee P (1994) The economic impact of musculoskeletal disorders. Qual Life Res 3(Suppl 1):S85–S91PubMedCrossRef

60.

Li WJ, Jiang YJ, Tuan RS (2006) Chondrocyte phenotype in engineered fibrous matrix is regulated by fiber size. Tissue Eng 12(7):1775–1785PubMedCrossRef

61.

Luk KD, Ruan DK, Chow DH, Leong JC (1997) Intervertebral disc autografting in a bipedal animal model. Clin Orthop Relat Res 337:13–26PubMedCrossRef

62.

Mani V, Adler E, Briley-Saebo KC, Bystrup A, Fuster V, Keller G et al (2008) Serial in vivo positive contrast MRI of iron oxide-labeled embryonic stem cell-derived cardiac precursor cells in a mouse model of myocardial infarction. Magn Reson Med 60(1):73–81PubMedCrossRef

63.

Masuda K, Aota Y, Muehleman C, Imai Y, Okuma M, Thonar EJ et al (2005) A novel rabbit model of mild, reproducible disc degeneration by an anulus needle puncture: correlation between the degree of disc injury and radiological and histological appearances of disc degeneration. Spine 30(1):5–14PubMed

64.

Masuda K, Imai Y, Okuma M, Muehleman C, Nakagawa K, Akeda K et al (2006) Osteogenic protein-1 injection into a degenerated disc induces the restoration of disc height and structural changes in the rabbit anular puncture model. Spine 31(7):742–754PubMedCrossRef

65.

Meisel HJ, Siodla V, Ganey TM, Minkus Y, Hutton WC, Alasevic OJ (2007) Clinical experience in cell-based therapeutics: disc chondrocyte transplantation. A treatment for degenerated or damaged intervertebral disc. Biomol Eng 24(1):5–21PubMedCrossRef

66.

Melrose J, Roberts S, Smith S, Menage J, Ghosh P (2002) Increased nerve and blood vessel ingrowth associated with proteoglycan depletion in an ovine anular lesion model of experimental disc degeneration. Spine 27(12):1278–1285PubMedCrossRef

67.

Menezes NM, Gray ML, Hartke JR, Burstein D (2004) T2 and T1rho MRI in articular cartilage systems. Magn Reson Med 51(3):503–509PubMedCrossRef

68.

Mignotte F, Champagne AM, Froger-Gaillard B, Benel L, Gueride M, Adolphe M et al (1991) Mitochondrial biogenesis in rabbit articular chondrocytes transferred to culture. Biol Cell 71(1–2):67–72PubMedCrossRef

69.

Miller JA, Schmatz C, Schultz AB (1988) Lumbar disc degeneration: correlation with age, sex, and spine level in 600 autopsy specimens. Spine 13(2):173–178PubMedCrossRef

70.

Mirza SK, Deyo RA (2007) Systematic review of randomized trials comparing lumbar fusion surgery to nonoperative care for treatment of chronic back pain. Spine 32(7):816–823PubMedCrossRef

71.

Mizuno H, Roy AK, Vacanti CA, Kojima K, Ueda M, Bonassar LJ (2004) Tissue-engineered composites of anulus fibrosus and nucleus pulposus for intervertebral disc replacement. Spine 29(12):1290–1297PubMedCrossRef

72.

Mizuno H, Roy AK, Zaporojan V, Vacanti CA, Ueda M, Bonassar LJ (2006) Biomechanical and biochemical characterization of composite tissue-engineered intervertebral discs. Biomaterials 27(3):362–370PubMedCrossRef

73.

Nachemson A (1960) Lumbar intradiscal pressure. Experimental studies on post-mortem material. Acta Orthop Scand Suppl 43:1–104PubMed

74.

Nachemson A, Lewin T, Maroudas A, Freeman MA (1970) In vitro diffusion of dye through the end-plates and the annulus fibrosus of human lumbar inter-vertebral discs. Acta Orthop Scand 41(6):589–607PubMedCrossRef

75.

Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T et al (2008) Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat Biotechnol 26(1):101–106PubMedCrossRef

76.

Nerurkar NL, Elliott DM, Mauck RL (2007) Mechanics of oriented electrospun nanofibrous scaffolds for annulus fibrosus tissue engineering. J Orthop Res 25(8):1018–1028PubMedCrossRef

77.

Nomura T, Mochida J, Okuma M, Nishimura K, Sakabe K (2001) Nucleus pulposus allograft retards intervertebral disc degeneration. Clin Orthop Relat Res 389:94–101PubMedCrossRef

78.

O’halloran DM, Pandit AS (2007) Tissue-engineering approach to regenerating the intervertebral disc. Tissue Eng 13(8):1927PubMedCrossRef

79.

Okuda S, Iwasaki M, Miyauchi A, Aono H, Morita M, Yamamoto T (2004) Risk factors for adjacent segment degeneration after PLIF. Spine 29(14):1535–1540PubMedCrossRef

80.

Okuma M, Mochida J, Nishimura K, Sakabe K, Seiki K (2000) Reinsertion of stimulated nucleus pulposus cells retards intervertebral disc degeneration: an in vitro and in vivo experimental study. J Orthop Res 18(6):988–997PubMedCrossRef

81.

Olson EJ, Hanley EN Jr, Rudert MJ, Baratz ME (1991) Vertebral column allografts for the treatment of segmental spine defects. An experimental investigation in dogs. Spine 16(9):1081–1088PubMedCrossRef

82.

Pezowicz CA, Robertson PA, Broom ND (2006) The structural basis of interlamellar cohesion in the intervertebral disc wall. J Anat 208(3):317–330PubMedCrossRef

83.

Putzier M, Schneider SV, Funk JF, Tohtz SW, Perka C (2005) The surgical treatment of the lumbar disc prolapse: nucleotomy with additional transpedicular dynamic stabilization versus nucleotomy alone. Spine 30(5):E109–E114PubMedCrossRef

84.

Rajasekaran S, Babu JN, Arun R, Armstrong BR, Shetty AP, Murugan S (2004) ISSLS prize winner: a study of diffusion in human lumbar discs: a serial magnetic resonance imaging study documenting the influence of the endplate on diffusion in normal and degenerate discs. Spine 29(23):2654–2667PubMedCrossRef

85.

Rajpurohit R, Risbud MV, Ducheyne P, Vresilovic EJ, Shapiro IM (2002) Phenotypic characteristics of the nucleus pulposus: expression of hypoxia inducing factor-1, glucose transporter-1 and MMP-2. Cell Tissue Res 308(3):401–407PubMedCrossRef

86.

Rapoport J, Jacobs P, Bell NR, Klarenbach S (2004) Refining the measurement of the economic burden of chronic diseases in Canada. Chronic Dis Can 24(4):13–21

87.

Ray CD (2002) The PDN prosthetic disc-nucleus device. Eur Spine J 11(Suppl 2):S137–S142PubMed

88.

Razaq S, Wilkins RJ, Urban JP (2003) The effect of extracellular pH on matrix turnover by cells of the bovine nucleus pulposus. Eur Spine J 12(4):341–349PubMedCrossRef

89.

Richardson SM, Mobasheri A, Freemont AJ, Hoyland JA (2007) Intervertebral disc biology, degeneration and novel tissue engineering and regenerative medicine therapies. Histol Histopathol 22(9):1033–1041PubMed

90.

Richardson SM, Walker RV, Parker S, Rhodes NP, Hunt JA, Freemont AJ et al (2006) Intervertebral disc cell-mediated mesenchymal stem cell differentiation. Stem Cells 24(3):707–716PubMedCrossRef

91.

Risbud MV, Albert TJ, Guttapalli A, Vresilovic EJ, Hillibrand AS, Vaccaro AR et al (2004) Differentiation of mesenchymal stem cells towards a nucleus pulposus-like phenotype in vitro: implications for cell-based transplantation therapy. Spine 29(23):2627–2632PubMedCrossRef

92.

Roberts S, Evans EH, Kletsas D, Jaffray DC, Eisenstein SM (2006) Senescence in human intervertebral discs. Eur Spine J 15(Suppl 3):S312–S316PubMedCrossRef

93.

Roberts S, Evans H, Trivedi J, Menage J (2006) Histology and pathology of the human intervertebral disc. J Bone Joint Surg Am 88(Suppl 2):10–14PubMedCrossRef

94.

Roughley P, Hoemann C, DesRosiers E, Mwale F, Antoniou J, Alini M (2006) The potential of chitosan-based gels containing intervertebral disc cells for nucleus pulposus supplementation. Biomaterials 27(3):388–396PubMedCrossRef

95.

Ruan D, He Q, Ding Y, Hou L, Li J, Luk KD (2007) Intervertebral disc transplantation in the treatment of degenerative spine disease: a preliminary study. Lancet 369(9566):993–999PubMedCrossRef

96.

Sahlman J, Inkinen R, Hirvonen T, Lammi MJ, Lammi PE, Nieminen J et al (2001) Premature vertebral endplate ossification and mild disc degeneration in mice after inactivation of one allele belonging to the Col2a1 gene for Type II collagen. Spine 26(23):2558–2565PubMedCrossRef

97.

Sakai D, Mochida J, Iwashina T, Hiyama A, Omi H, Imai M et al (2006) Regenerative effects of transplanting mesenchymal stem cells embedded in atelocollagen to the degenerated intervertebral disc. Biomaterials 27(3):335–345PubMedCrossRef

98.

Sakai D, Mochida J, Iwashina T, Watanabe T, Nakai T, Ando K et al (2005) Differentiation of mesenchymal stem cells transplanted to a rabbit degenerative disc model: potential and limitations for stem cell therapy in disc regeneration. Spine 30(21):2379–2387PubMedCrossRef

99.

Sato M, Asazuma T, Ishihara M, Ishihara M, Kikuchi T, Kikuchi M et al (2003) An experimental study of the regeneration of the intervertebral disc with an allograft of cultured annulus fibrosus cells using a tissue-engineering method. Spine 28(6):548–553PubMedCrossRef

100.

Schallmoser K, Bartmann C, Rohde E, Reinisch A, Kashofer K, Stadelmeyer E et al (2007) Human platelet lysate can replace fetal bovine serum for clinical-scale expansion of functional mesenchymal stromal cells. Transfusion 47(8):1436–1446PubMedCrossRef

101.

Seguin CA, Grynpas MD, Pilliar RM, Waldman SD, Kandel RA (2004) Tissue engineered nucleus pulposus tissue formed on a porous calcium polyphosphate substrate. Spine 29(12):1299–1306PubMedCrossRef

102.

Shahdadfar A, Fronsdal K, Haug T, Reinholt FP, Brinchmann JE (2005) In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability. Stem Cells 23(9):1357–1366PubMedCrossRef

103.

Shim CS, Lee SH, Shin HD, Kang HS, Choi WC, Jung B et al (2007) CHARITE versus ProDisc: a comparative study of a minimum 3-year follow-up. Spine 32(9):1012–1018PubMedCrossRef

104.

Simon SR et al (1994) Kinesiology. In: Simon SR (ed) Orthopedic Basic Science. Am Academy of Orthopedic Surgeons, USA, pp 558–68

105.

Sivan SS, Tsitron E, Wachtel E, Roughley P, Sakkee N, van der Ham F et al (2006) Age-related accumulation of pentosidine in aggrecan and collagen from normal and degenerate human intervertebral discs. Biochem J 399(1):29–35PubMedCrossRef

106.

Sivan SS, Wachtel E, Tsitron E, Sakkee N, van der Ham F, Degroot J et al (2008) Collagen turnover in normal and degenerate human intervertebral discs as determined by the racemization of aspartic acid. J Biol Chem 283(14):8796–8801PubMedCrossRef

107.

So K, Takemoto M, Fujibayashi S, Neo M, Kyomoto M, Hayami T et al (2007) Antidegenerative effects of partial disc replacement in an animal surgery model. Spine 32(15):1586–1591PubMedCrossRef

108.

Sobajima S, Vadala G, Shimer A, Kim JS, Gilbertson LG, Kang JD (2007) Feasibility of a stem cell therapy for intervertebral disc degeneration. Spine J (in press)

109.

Solovieva S, Lohiniva J, Leino-Arjas P, Raininko R, Luoma K, la-Kokko L et al (2002) COL9A3 gene polymorphism and obesity in intervertebral disc degeneration of the lumbar spine: evidence of gene-environment interaction. Spine 27(23):2691–2696PubMedCrossRef

110.

Stairmand JW, Holm S, Urban JP (1991) Factors influencing oxygen concentration gradients in the intervertebral disc. A theoretical analysis. Spine 16(4):444–449PubMedCrossRef

111.

Steck E, Bertram H, Abel R, Chen B, Winter A, Richter W (2005) Induction of intervertebral disc-like cells from adult mesenchymal stem cells. Stem Cells 23(3):403–411PubMedCrossRef

112.

Sun Y, Hurtig M, Pilliar RM, Grynpas M, Kandel RA (2001) Characterization of nucleus pulposus-like tissue formed in vitro. J Orthop Res 19(6):1078–1084PubMedCrossRef

113.

Sutton EJ, Henning TD, Pichler BJ, Bremer C, drup-Link HE (2008) Cell tracking with optical imaging. Eur Radiol 18(10):2021–2032

114.

Trout JJ, Buckwalter JA, Moore KC, Landas SK (1982) Ultrastructure of the human intervertebral disc. I. Changes in notochordal cells with age. Tissue Cell 14(2):359–369PubMedCrossRef

115.

Urban JP, Roberts S (2003) Degeneration of the intervertebral disc. Arthritis Res Ther 5(3):120–130PubMedCrossRef

116.

Urban JP, Smith S, Fairbank JC (2004) Nutrition of the intervertebral disc. Spine 29(23):2700–2709PubMedCrossRef

117.

Urban MR, Fairbank JC, Bibby SR, Urban JP (2001) Intervertebral disc composition in neuromuscular scoliosis: changes in cell density and glycosaminoglycan concentration at the curve apex. Spine 26(6):610–617PubMedCrossRef

118.

Vadala G, Studer RK, Sowa G, Spiezia F, Iucu C, Denaro V et al (2008) Coculture of bone marrow mesenchymal stem cells and nucleus pulposus cells modulate gene expression profile without cell fusion. Spine 33(8):870–876PubMedCrossRef

119.

Vernengo J, Fussell GW, Smith NG, Lowman AM (2007) Evaluation of novel injectable hydrogels for nucleus pulposus replacement. J Biomed Mater Res B Appl Biomater 84(1):64–69

120.

Virtanen IM, Karppinen J, Taimela S, Ott J, Barral S, Kaikkonen K et al (2007) Occupational and genetic risk factors associated with intervertebral disc disease. Spine 32(10):1129–1134PubMedCrossRef

121.

Waldman SD, Grynpas MD, Pilliar RM, Kandel RA (2002) Characterization of cartilagenous tissue formed on calcium polyphosphate substrates in vitro. J Biomed Mater Res 62(3):323–330PubMedCrossRef

122.

Wang Y, Huso DL, Harrington J, Kellner J, Jeong DK, Turney J et al (2005) Outgrowth of a transformed cell population derived from normal human BM mesenchymal stem cell culture. Cytotherapy 7(6):509–519PubMedCrossRef

123.

Wilke HJ, Neef P, Caimi M, Hoogland T, Claes LE (1999) New in vivo measurements of pressures in the intervertebral disc in daily life. Spine 24(8):755–762PubMedCrossRef

124.

Yamanaka S (2007) Strategies and new developments in the generation of patient-specific pluripotent stem cells. Cell Stem Cell 1(1):39–49PubMedCrossRef

125.

Zhang YG, Guo X, Xu P, Kang LL, Li J (2005) Bone mesenchymal stem cells transplanted into rabbit intervertebral discs can increase proteoglycans. Clin Orthop Relat Res 430:219–226PubMedCrossRef

126.

Zigler J, Delamarter R, Spivak JM, Linovitz RJ, Danielson GOIII, Haider TT et al (2007) Results of the prospective, randomized, multicenter Food and Drug Administration investigational device exemption study of the ProDisc-L total disc replacement versus circumferential fusion for the treatment of 1-level degenerative disc disease. Spine 32(11):1155–1162PubMedCrossRef

127.

Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13(12):4279–4295PubMedCrossRef

Title: Tissue engineering and the intervertebral disc: the challenges
Authors: Rita Kandel
Sally Roberts
Jill P. G. Urban
Publication date: 01-12-2008
Publisher: Springer-Verlag
Published in: European Spine Journal / Issue Special Issue 4/2008
Print ISSN: 0940-6719
Electronic ISSN: 1432-0932
DOI: https://doi.org/10.1007/s00586-008-0746-2

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Tissue engineering and the intervertebral disc: the challenges

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Other articles of this Special Issue 4/2008

Resolving discogenic pain

Biological repair of the degenerated intervertebral disc by the injection of growth factors

New concept for backache: biopsychosocial pain syndrome

World Forum for Spine Research: The intervertebral disc

Cell transplantation in lumbar spine disc degeneration disease

Intervertebral disc transplantation: a biological approach to motion preservation