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Journal of Anesthesia

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01-06-2016 | Original Article

Role of MnSOD in propofol protection of human umbilical vein endothelial cells injured by heat stress

Authors: F. Wu, X. J. Dong, H. Q. Zhang, L. Li, Q. L. Xu, Z. F. Liu, Z. T. Gu, L. Su

Published in: Journal of Anesthesia | Issue 3/2016

Abstract

Purpose

Heat stress stimulation can cause various injuries in human umbilical vein endothelial cells (HUVEC), including apoptotic cell death and an increase in cell permeability. Propofol (PPF), a commonly used anesthetic, is known to have an important role in antioxidation as well as organ protection. Therefore, our aim is to evaluate the protective effects of PPF on heat stress (HS)-induced oxidative stress injury and its possible mechanism of action.

Methods

For HS + PPF, cells were treated with propofol followed by 2 h heat stress at 43 °C and then 4 h incubation under normal conditions. For propofol treatment, HUVEC were cultured in serum-free Dulbecco’s modified Eagle medium supplemented with 0, 10, 25, or 50 μM propofol for 6 h under normal conditions.

Results

During the study, we found that, in HS-induced cellular damage, the protective effect of propofol was related closely with its antioxidation properties. We further revealed that heat stress significantly reduced the level of manganese superoxide demutase (MnSOD) and Cu/Zn SOD, but that propofol could inhibit the reduction of MnSOD only. Transfection of HUVEC with MnSOD small interfering RNA (siRNA) markedly decreased the expression of MnSOD, and the protective effect of propofol in the MnSOD siRNA clones was significantly reduced.

Conclusion

Propofol protected the heat stress-injured cells, at least partly, through upregulating MnSOD expression, effectively reducing the direct or indirect cell damage caused by oxidative stress.

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Title: Role of MnSOD in propofol protection of human umbilical vein endothelial cells injured by heat stress
Authors: F. Wu
X. J. Dong
H. Q. Zhang
L. Li
Q. L. Xu
Z. F. Liu
Z. T. Gu
L. Su
Publication date: 01-06-2016
Publisher: Springer Japan
Published in: Journal of Anesthesia / Issue 3/2016
Print ISSN: 0913-8668
Electronic ISSN: 1438-8359
DOI: https://doi.org/10.1007/s00540-015-2129-2

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Role of MnSOD in propofol protection of human umbilical vein endothelial cells injured by heat stress

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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