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Intensive Care Medicine

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01-09-2003 | Experimental

Effects of hypertonic (3%) saline in rats with circulatory shock and cerebral ischemia after heatstroke

Authors: Jinn-Rung Kuo, Chia-Li Lin, Chung-Ching Chio, Jhi-Joung Wang, Mao-Tsun Lin

Published in: Intensive Care Medicine | Issue 9/2003

Abstract

Objective

To evaluate the effects of hypertonic (3%) saline in heatstroke rats with circulatory shock, intracranial hypertension, and cerebral ischemia.

Design and setting

Urethane-anesthetized rats were exposed to a high ambient temperature of 42°C until mean arterial pressure and local cerebral blood flow (CBF) in the corpus striatum began to decrease from their peak levels, which was arbitrarily defined as the onset of heatstroke. Control rats were exposed to 24°C.

Measurements and results

Extracellular concentrations of glutamate and lactate/pyruvate ratio (cellular ischemia markers), and glycerol (a cellular injury marker) in the corpus striatum of rat brain were assessed by intracerebral microdialysis methods. Striatal PO₂, temperature, and local CBF were measured with a combined OxyLite PO₂, thermocouple, and OxyFlo LDF, respectively. The values of mean arterial pressure, cerebral perfusion pressure, and striatal CBF and PO₂ in rats treated with 0.9% NaCl solution after the onset of heatstroke were all significantly lower than those in normothermic controls. In contrast, the values of intracranial pressure, brain temperature, and extracellular concentrations of glutamate, glycerol, and lactate/pyruvate in the corpus striatum were greater. Intravenous infusion of hypertonic (3%) saline solution either "0" time before the start of heat exposure or right after the onset of heatstroke significantly attenuated the heatstroke-induced arterial hypotension, intracranial hypertension, decreased cerebral perfusion, and cerebral ischemia and damage and resulted in prolongation of survival time.

Conclusions

Our results strongly suggest that the experimental heatstroke syndromes can be effectively prevented and treated by hypertonic saline.

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Title: Effects of hypertonic (3%) saline in rats with circulatory shock and cerebral ischemia after heatstroke
Authors: Jinn-Rung Kuo
Chia-Li Lin
Chung-Ching Chio
Jhi-Joung Wang
Mao-Tsun Lin
Publication date: 01-09-2003
Publisher: Springer-Verlag
Published in: Intensive Care Medicine / Issue 9/2003
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-003-1854-4

At a glance: The STEP trials

Springer Medicine

Effects of hypertonic (3%) saline in rats with circulatory shock and cerebral ischemia after heatstroke

Abstract

Objective

Design and setting

Measurements and results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Design and setting

Measurements and results

Conclusions

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