Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

ACC 2024 Congress

Catch up on all the top stories and latest evidence in cardiology research with our ACC 2024 Congress hub page.
ADVANCED SEARCH
Log in
Top
Journal of Gastroenterology
Published in: Journal of Gastroenterology 3/2018

01-03-2018 | Original Article—Alimentary Tract

Novel probiotics isolated from a Japanese traditional fermented food, Funazushi, attenuates DSS-induced colitis by increasing the induction of high integrin αv/β8-expressing dendritic cells

Authors: Yoshikiyo Okada, Yoshikazu Tsuzuki, Takajo Takeshi, Hirotaka Furuhashi, Masaaki Higashiyama, Chikako Watanabe, Kazuhiko Shirakabe, Chie Kurihara, Shunsuke Komoto, Kengo Tomita, Shigeaki Nagao, Soichiro Miura, Ryota Hokari

Published in: Journal of Gastroenterology | Issue 3/2018

Login to get access

Abstract

Background

We isolated two novel probiotics strains (s193 and s292) from Funazushi, which is a traditional Japanese fermented food, and evaluated its effects on DSS-induced colitis to determine the possible underlying mechanisms.

Methods

A single colony from homogenized Funazushi was isolated by its ability to suppress TNF-α in RAW 264.7. Effect of probiotics on colonic inflammation induced by DSS was evaluated. Effect of probiotics on Treg induction by CD11c+ dendritic cells (DCs) of MLNs were analyzed.

Results

Two novel probiotics strains classified into the genus Lactobacillus were isolated (s193 and s292), and those strains showed stronger anti-inflammatory effects on DSS-induced colitis than those of L. gasseri isolated from the gut. mRNA expression β8 integrin in CD11c+DCs of MLNs and the number of Tregs in the large intestine were significantly increased by s193 and s292 administration compared with L. gasseri administration. Bone marrow DCs treated with s193 and s292 highly increased β8 integrin, and those cells strongly induced differentiation of CD4+ T cells into Tregs. Differentiation of Tregs was remarkably inhibited by anti-β8 integrin antibody treatment.

Conclusions

Strains s193 and s292 demonstrate strong anti-inflammatory effects on DSS-induced colitis through induction of β8 integrin expression on DCs. Our results suggested that Japanese traditional fermented foods are valuable sources for probiotics that are effective for IBD therapy and treatment.
Literature
1.
Ghouri YA, Richards DM, Rahimi EF, et al. Systematic review of randomized controlled trials of probiotics, prebiotics, and synbiotics in inflammatory bowel disease. Clin Exp Gastroenterol. 2014;7:473–87 (epub 2014/12/20).PubMedPubMedCentral
2.
Sanders ME, Guarner F, Guerrant R, et al. An update on the use and investigation of probiotics in health and disease. Gut. 2013;62(5):787–96 (epub 2013/03/12).CrossRefPubMedPubMedCentral
3.
Lievin-Le Moal V, Servin AL. Anti-infective activities of Lactobacillus strains in the human intestinal microbiota: from probiotics to gastrointestinal anti-infectious biotherapeutic agents. Clin Microbiol Rev. 2014;27(2):167–99 (epub 2014/04/04).CrossRefPubMedPubMedCentral
4.
Khanghah SM, Ganbarov K. Lactobacillus with probiotic potential from homemade cheese in Azerbijan. Bioimpacts. 2014;4(1):49–52 (epub 2014/05/03).
5.
Swain MR, Anandharaj M, Ray RC, et al. Fermented fruits and vegetables of Asia: a potential source of probiotics. Biotechnol Res Int. 2014;2014:250424 (epub 2014/10/25).CrossRefPubMedPubMedCentral
6.
Murooka Y, Yamshita M. Traditional healthful fermented products of Japan. J Ind Microbiol Biotechnol. 2008;35(8):791–8 (epub 2008/05/08).CrossRefPubMed
7.
Tsuda H, Kubota K, Mtsumoto T, Ishim Y. Isolation and identification of lactic acid bacteria in traditional fermented sushi, Funazushi, from Japan. Food Sci Technol Res. 2012;18(1):77–82.CrossRef
8.
Ohsima N, Fujii T. Effect of administered Lactobacillus isolated from “Funazushi” on fecal microflora of rats. Jpn Soc Food Microbiol. 1994;11(2):129–32.CrossRef
9.
Isobe Y, Mizuhashi T, Narita M. Microbial flora of Funazushi (Pickled Crucian Carp). J Home Econ Jpn. 2002;53(1):61–4.
10.
11.
Jeon SG, Kayama H, Ueda Y, et al. Probiotic Bifidobacterium breve induces IL-10-producing Tr1 cells in the colon. PLoS Pathog. 2012;8(5):e1002714 (epub 2012/06/14).CrossRefPubMedPubMedCentral
12.
Fernandez EM, Valenti V, Rockel C, et al. Anti-inflammatory capacity of selected Lactobacilli in experimental colitis is driven by NOD2-mediated recognition of a specific peptidoglycan-derived muropeptide. Gut. 2011;60(8):1050–9 (epub 2011/04/08).CrossRefPubMed
13.
14.
Okada Y, Tsuzuki Y, Hokari R, et al. Anti-inflammatory effects of the genus Bifidobacterium on macrophages by modification of phospho-I kappaB and SOCS gene expression. Int J Exp Pathol. 2009;90(2):131–40 (epub 2009/04/02).CrossRefPubMedPubMedCentral
15.
Osman N, Adawi D, Ahrne S, et al. Modulation of the effect of dextran sulfate sodium-induced acute colitis by the administration of different probiotic strains of Lactobacillus and Bifidobacterium. Dig Dis Sci. 2004;49(2):320–7 (epub 2004/04/24).CrossRefPubMed
16.
Schmidt RJ, Emara MG, Kung L Jr. The use of a quantitative real-time polymerase chain reaction assay for identification and enumeration of Lactobacillus buchneri in silage. J Appl Microbiol. 2008;105(3):920–9.CrossRefPubMed
17.
Lutz MB, Kukutsch N, Ogilvie AL, et al. An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J Immunol Methods. 1999;223(1):77–92 (epub 1999/02/26).CrossRefPubMed
18.
Ivanov II, Frutos Rde L, Manel N, et al. Specific microbiota direct the differentiation of IL-17-producing T-helper cells in the mucosa of the small intestine. Cell Host Microbe. 2008;4(4):337–49 (epub 2008/10/16).CrossRefPubMedPubMedCentral
19.
Matsunaga H, Hokari R, Ueda T, et al. Physiological stress exacerbates murine colitis by enhancing proinflammatory cytokine expression that is dependent on IL-18. Am J Physiol Gastrointest Liver Physiol. 2011;301(3):G555–64.CrossRefPubMed
20.
Worthington JJ, Czajkowska BI, Melton AC, et al. Intestinal dendritic cells specialize to activate transforming growth factor-beta and induce Foxp3 + regulatory T cells via integrin αvβ8. Gastroenterology. 2011;141(5):1802–12.CrossRefPubMedPubMedCentral
21.
Paidassi H, Acharya M, Zhang A, et al. Preferential expression of integrin αvβ8 promotes generation of regulatory T cells by mouse CD103 + dendritic cells. Gastroenterology. 2011;141(5):1813–20.CrossRefPubMedPubMedCentral
22.
Round JL, Mazmanian SK. The gut microbiota shapes intestinal immune responses during health and disease. Nat Rev Immunol. 2009;9(5):313–23 (epub 2009/04/04).CrossRefPubMedPubMedCentral
23.
Macia L, Thorburn AN, Binge LC, et al. Microbial influences on epithelial integrity and immune function as a basis for inflammatory diseases. Immunol Rev. 2012;245(1):164–76 (epub 2011/12/16).CrossRefPubMed
24.
Elmer GW. Probiotics: “living drugs”. Am J Health Syst Pharm. 2001;58(12):1101–9.PubMed
25.
Goldsmith JR, Sartor RB. Response to the letter by Brotherton regarding “insoluble fiber and intestinal microbiota metabolism”. J Gastroenterol. 2015;50(4):492–3.CrossRefPubMed
26.
Sun M, Wu W, Liu Z, Cong Y, et al. Microbiota metabolite short chain fatty acids, GPCR, and inflammatory bowel diseases. J Gastroenterol. 2017;52(1):1–8 (epub 2016 Jul 23).CrossRefPubMed
27.
Uchiyama K, Odahara S, Nakamura M, et al. The fatty acid profile of the erythrocyte membrane in initial-onset inflammatory bowel disease patients. Dig Dis Sci. 2013;58(5):1235–43 (epub 2013/01/12).CrossRefPubMed
28.
Ozawa N, Shimojo N, Suzuki Y, et al. Maternal intake of Natto, a Japan’s traditional fermented soybean food, during pregnancy and the risk of eczema in Japanese babies. Allergol Int. 2014;63(2):261–6 (epub 2014/04/25).CrossRefPubMed
29.
Takeda E, Sakamoto K, Yokota K, et al. Phosphorus supply per capita from food in Japan between 1960 and 1995. J Nutr Sci Vitaminol (Tokyo). 2002;48(2):102–8 (epub 2002/08/13).CrossRefPubMed
30.
Kanai T, Matsuoka K, Naganuma M, et al. Diet, microbiota, and inflammatory bowel disease: lessons from Japanese foods. Korean J Intern Med. 2014;29(4):409–15 (epub 2014/07/22).CrossRefPubMedPubMedCentral
31.
Mariko M, Nahoko U, Yuka I, et al. Effects on preferability of the experience in tasting Funazushi and information about the food. J Home Econ Jpn. 2012;63(11):737–44.
32.
Asakura K, Nishiwaki Y, Inoue N, et al. Prevalence of ulcerative colitis and Crohn’s disease in Japan. J Gastroenterol. 2009;44(7):659–65 (epub 2009/05/09).CrossRefPubMed
33.
Sheehan D, Moran C, Shanahan F. The microbiota in inflammatory bowel disease. J Gastroenterol. 2015;50(5):495–507 (epub 2015 Mar 26).CrossRefPubMed
34.
Boucard-Jourdin M, Kugler D, Endale Ahanda ML, et al. β8 integrin expression and activation of TGF-α by intestinal dendritic cells are determined by both tissue microenvironment and cell lineage. J Immunol. 2016;197(5):1968–78.CrossRefPubMedPubMedCentral
Metadata
Title
Novel probiotics isolated from a Japanese traditional fermented food, Funazushi, attenuates DSS-induced colitis by increasing the induction of high integrin αv/β8-expressing dendritic cells
Authors
Yoshikiyo Okada
Yoshikazu Tsuzuki
Takajo Takeshi
Hirotaka Furuhashi
Masaaki Higashiyama
Chikako Watanabe
Kazuhiko Shirakabe
Chie Kurihara
Shunsuke Komoto
Kengo Tomita
Shigeaki Nagao
Soichiro Miura
Ryota Hokari
Publication date
01-03-2018
Publisher
Springer Japan
Published in
Journal of Gastroenterology / Issue 3/2018
Print ISSN: 0944-1174
Electronic ISSN: 1435-5922
DOI
https://doi.org/10.1007/s00535-017-1362-x

Other articles of this Issue 3/2018

Journal of Gastroenterology 3/2018 Go to the issue

Original Article—Liver, Pancreas, and Biliary Tract

Combination of PNPLA3 and TLL1 polymorphism can predict advanced fibrosis in Japanese patients with nonalcoholic fatty liver disease

Acknowledgment

Acknowledgements

Original Article―Alimentary Tract

A multicenter, retrospective, observational study of the clinical outcomes and risk factors for relapse of ulcerative colitis at 1 year after leukocytapheresis

Special Article

Evidence-based clinical practice guidelines for inflammatory bowel disease

Original Article—Alimentary Tract

Comparison of propofol with midazolam in endoscopic submucosal dissection for esophageal squamous cell carcinoma: a randomized controlled trial

Original Article—Liver, Pancreas, and Biliary Tract

Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis

ACC 2024 Congress Coverage

Cardiology Video

Highlights from the ACC 2024 Congress

Watch now

Watch official videos from the ACC congress summarizing key highlights from the last year in heart failure, cardiomyopathies, valvular disease, pulmonary vascular disease, and pediatric cardiology.

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits