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Journal of Cancer Research and Clinical Oncology

Published in:

01-10-2010 | Original Paper

Identification of proteins responsible for the multiple drug resistance in 5-fluorouracil-induced breast cancer cell using proteomics analysis

Authors: Guopei Zheng, Fang Peng, Renkui Ding, Yanhui Yu, Yongmei Ouyang, Zhuchu Chen, Zhiqiang Xiao, Zhimin He

Published in: Journal of Cancer Research and Clinical Oncology | Issue 10/2010

Abstract

Purpose

This study aimed to explore the mechanism of multi-drug resistance (MDR) in 5-fluorouracil (5-FU)-induced breast cancer cell MCF-7.

Methods

MCF-7 cells were exposed in stepwise escalating concentration of 5-FU to develop the resistant cell line, MCF-7/5-FU. Biological and molecular characteristics of the cells were studied through MTT, flow cytometry, real-time PCR, western-blot, and the global protein profiles between MCF-7/5-FU and parental MCF-7 were compared using proteomic approach. Then some of the differentially expressed proteins were validated by western-blot. In addition, the role of 14-3-3σ was validated using gene transfection.

Results

Drug resistance of MCF-7/5-FU cells to 5-FU, MX, cDDP, ADM, TAXOL all increased significantly compared with MCF-7 cells and that maybe related to BCRP, but not MDR1 and MRP1. Differentially expressed proteins between MCF-7/5-FU and MCF-7 cells were identified; 12 proteins were up-regulated and 18 proteins were down-regulated in MCF-7/5-FU cells. Expressive levels of some proteins in western-blot validation were consistent with the results in proteomic analysis. Enforced 14-3-3σ expression can increase the sensitivity of MCF-7/5-FU cells to 5-FU and cDDP.

Conclusion

MDR of MCF-7/5-FU likely associated with differentially expressed proteins and 14-3-3σ may play a positive role in chemotherapy. These findings may provide theoretical support for the prediction of chemotherapeutic response and reverse of MDR.

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Title: Identification of proteins responsible for the multiple drug resistance in 5-fluorouracil-induced breast cancer cell using proteomics analysis
Authors: Guopei Zheng
Fang Peng
Renkui Ding
Yanhui Yu
Yongmei Ouyang
Zhuchu Chen
Zhiqiang Xiao
Zhimin He
Publication date: 01-10-2010
Publisher: Springer-Verlag
Published in: Journal of Cancer Research and Clinical Oncology / Issue 10/2010
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-010-0805-z

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Identification of proteins responsible for the multiple drug resistance in 5-fluorouracil-induced breast cancer cell using proteomics analysis

Abstract

Purpose

Methods

Results

Conclusion

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Abstract

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