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Journal of Cancer Research and Clinical Oncology

Published in:

01-03-2010 | Original Paper

Expression of multidrug resistance-associated ABC transporters in B-CLL is independent of ZAP70 status

Authors: Alexander Hoellein, Thomas Decker, Christian Bogner, Madlen Oelsner, Stefanie Hauswald, Christian Peschel, Ulrich Keller, Thomas Licht

Published in: Journal of Cancer Research and Clinical Oncology | Issue 3/2010

Abstract

Purpose

To assess whether the poor prognosis of ZAP70-positive B-cell chronic lymphocytic leukemia (CLL) is associated with the overexpression of ABC transporter genes that are responsible for pleiotropic drug resistance.

Materials and methods

The transcript level of ten drug transporters was analyzed using semiquantitative and quantitative RT-PCR in control hematopoietic cells, in 41 CLL patient samples and in 5 lymphoma cell lines. ZAP70 status was determined by immunoblotting.

Results

Of all analyzed transporters, MDR1, MDR2, MRP1, MRP4, MRP5, and MRP7 were expressed at a significantly higher level in B lymphocytes when compared with other hematopoietic cells in peripheral blood. A subgroup of 41 CLL patient samples showed similar or higher expression of these genes than control B cells, and CLL cells exhibited high expression when compared with multiple lymphoma cell lines. No significant correlation between ZAP70 expression and ABC transporter expression was observed.

Conclusion

The ZAP70 status is independent of the multidrug resistance phenotype in CLL.

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Title: Expression of multidrug resistance-associated ABC transporters in B-CLL is independent of ZAP70 status
Authors: Alexander Hoellein
Thomas Decker
Christian Bogner
Madlen Oelsner
Stefanie Hauswald
Christian Peschel
Ulrich Keller
Thomas Licht
Publication date: 01-03-2010
Publisher: Springer-Verlag
Published in: Journal of Cancer Research and Clinical Oncology / Issue 3/2010
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-009-0670-9

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Expression of multidrug resistance-associated ABC transporters in B-CLL is independent of ZAP70 status

Abstract

Purpose

Materials and methods

Results

Conclusion

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