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Journal of Cancer Research and Clinical Oncology

Published in:

01-09-2009 | Original Paper

A novel role for DYX1C1, a chaperone protein for both Hsp70 and Hsp90, in breast cancer

Authors: Yuxin Chen, Muzi Zhao, Saiqun Wang, Jie Chen, Yun Wang, Qinhong Cao, Wenbin Zhou, Jin Liu, Zhiyang Xu, Guoqing Tong, Jianmin Li

Published in: Journal of Cancer Research and Clinical Oncology | Issue 9/2009

Abstract

Aims

With three consecutive tetratricopeptide repeat (TPR) motifs at its C-terminus essential for neuronal migration, and a p23 domain at its N-terminus, DYX1C1 was the first gene proposed to have a role in developmental dyslexia. In this study, we attempted to identify the potential interaction of DYX1C1 and heat shock protein, and the role of DYX1C1 in breast cancer.

Main methods

GST pull-down, a yeast two-hybrid system, RT-PCR, site-directed mutagenesis approach.

Key findings

Our study initially confirmed DYX1C1, a dyslexia related protein, could interact with Hsp70 and Hsp90 via GST pull-down and a yeast two-hybrid system. And we verified that EEVD, the C-terminal residues of DYX1C1, is responsible for the identified association. Further, DYX1C1 mRNA was significantly overexpressed in malignant breast tumor, linking with the up-regulated expression of Hsp70 and Hsp90.

Significance

These results suggest that DYX1C1 is a novel Hsp70 and Hsp90-interacting co-chaperone protein and its expression is associated with malignancy.

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Title: A novel role for DYX1C1, a chaperone protein for both Hsp70 and Hsp90, in breast cancer
Authors: Yuxin Chen
Muzi Zhao
Saiqun Wang
Jie Chen
Yun Wang
Qinhong Cao
Wenbin Zhou
Jin Liu
Zhiyang Xu
Guoqing Tong
Jianmin Li
Publication date: 01-09-2009
Publisher: Springer-Verlag
Published in: Journal of Cancer Research and Clinical Oncology / Issue 9/2009
Print ISSN: 0171-5216
Electronic ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-009-0568-6

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A novel role for DYX1C1, a chaperone protein for both Hsp70 and Hsp90, in breast cancer

Abstract

Aims

Main methods

Key findings

Significance

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Abstract

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Main methods

Key findings

Significance

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