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01-04-2019 | Tuberculosis | Original Investigation

Diagnostic performance in active TB of QFT-Plus assay and co-expression of CD25/CD134 in response to new antigens of Mycobacterium tuberculosis

Authors: Ilaria Sauzullo, Fabio Mengoni, Claudia Mascia, Paolo Pavone, Giulia Savelloni, Anna Paola Massetti, Miriam Lichtner, Vincenzo Vullo, Claudio M. Mastroianni

Published in: Medical Microbiology and Immunology | Issue 2/2019

Abstract

The new QuantiFERON-TB Gold Plus employs modified peptides optimized to elicit an IFNγ response from CD8⁺ cytotoxic T lymphocytes in addition to CD4⁺ T cells. With a view to improve the difficult identification of TB cases, we assessed the combination of two specific immunological markers comprising IFNγ secretion and T cells co-expression of CD25 and CD134 in response to Mycobacterium tuberculosis-specific antigens. A total of 34 subjects with suspected TB and 10 age-matched HD were prospectively enrolled. Assessing the performance of QFT-Plus in terms of the TB1 and TB2 results, we found that in TB patients, the quantitative IFNγ value in TB2 was similar to that in TB1, and we did not find any differences irrespective of the disease (pulmonary or extra-pulmonary). The flow cytometric CD25/CD134 assay, allowed a more accurate differentiation between M. tuberculosis-infected and uninfected patients, with a better combination of sensitivity and specificity, especially by evaluation of CD4⁺ T-cell subset. All individuals with negative QFT-Plus results displayed a positive CD25/CD134 response. Overall, a positive correlation was found between T cells co-expressing CD25/CD134 and IFNγ levels in response to both QFT-Plus TB antigen tubes, as well as between the QFT-Plus TB1 and TB2 tubes. We demonstrated that both TB1 and TB2 induce a higher expression of CD25⁺CD134⁺ markers on CD4⁺ T cells among infected TB subjects, compared to the lower degree of CD8⁺ T cells, mainly induced to TB2 stimulation. We suggest that a combined use of classic QFT-Plus and specific CD25/CD134 response may be a useful means in the diagnostic workup for active TB.

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Title: Diagnostic performance in active TB of QFT-Plus assay and co-expression of CD25/CD134 in response to new antigens of Mycobacterium tuberculosis
Authors: Ilaria Sauzullo
Fabio Mengoni
Claudia Mascia
Paolo Pavone
Giulia Savelloni
Anna Paola Massetti
Miriam Lichtner
Vincenzo Vullo
Claudio M. Mastroianni
Publication date: 01-04-2019
Publisher: Springer Berlin Heidelberg
Keywords: Tuberculosis
Mycobacterium Tuberculosis
Tuberculosis
Published in: Medical Microbiology and Immunology / Issue 2/2019
Print ISSN: 0300-8584
Electronic ISSN: 1432-1831
DOI: https://doi.org/10.1007/s00430-018-00576-4

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