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01-05-2007 | Original Article

Genetic analysis of three US population groups using an X-chromosomal STR decaplex

Authors: Iva Gomes, Mechthild Prinz, Rui Pereira, Carole Meyers, Rebecca S. Mikulasovich, António Amorim, Angel Carracedo, Leonor Gusmão

Published in: International Journal of Legal Medicine | Issue 3/2007

Abstract

An X-chromosomal multiplex amplifying ten short tandem repeats (STRs) in one single PCR reaction was developed and optimized in this work. The X-STRs included were DXS8378, DXS9898, DXS8377, HPRTB, GATA172D05, DXS7423, DXS6809, DXS7132, DXS101, and DXS6789. Decaplex performance was tested on 377 male samples from three United States population groups, namely, 130 African Americans, 104 Asians, and 143 Hispanics. DXS8377 was the most polymorphic locus across all three populations, whereas DXS7423 was the least informative marker. Genetic distance analysis (R _ST and F _ST) performed for the three populations residing in New York showed significant genetic distances between population groups for most pairwise comparisons, except for HPRTB, DXS6809, and DXS7132. When testing linkage disequilibrium for all pairs of loci in the three groups, no significant association was found between any pair of the loci studied, after applying Bonferroni correction. The high values for the average probability of excluding a random man obtained in all three populations when both mother and daughter are tested or when father/daughter relationships are evaluated support the potential of this decaplex system in kinship analysis. Also, the overall high power of discrimination values for samples of female and male origin, confirms the usefulness of this decaplex system in identification analysis. As expected, results also support the use of independent databases comprising these ten X-linked loci for the three US populations evaluated.

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Title: Genetic analysis of three US population groups using an X-chromosomal STR decaplex
Authors: Iva Gomes
Mechthild Prinz
Rui Pereira
Carole Meyers
Rebecca S. Mikulasovich
António Amorim
Angel Carracedo
Leonor Gusmão
Publication date: 01-05-2007
Publisher: Springer-Verlag
Published in: International Journal of Legal Medicine / Issue 3/2007
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-006-0146-2

At a glance: The STEP trials

Springer Medicine

Genetic analysis of three US population groups using an X-chromosomal STR decaplex

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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