Top

Published in:

01-09-2006 | Short Communication

Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample

Authors: C. Robino, A. Giolitti, S. Gino, C. Torre

Published in: International Journal of Legal Medicine | Issue 5/2006

Abstract

Two multiplex polymerase chain reaction systems for the automated profiling of 12 X-chromosomal short tandem repeat (STR) markers were developed. Multiplex A consisted of DXS6789, DXS6809, GATA172D05, DXS101, DXS8378, and DXS8377. Multiplex B consisted of DXS7132, DXS6800, DXS6801, DXS7424, HPRTB, and DXS10011. The set of amplified X-STRs was designed to include groups of closely linked markers (DXS101–DXS7424 and DXS6789–DXS6801–DXS6809) to generate highly informative haplotypes for kinship testing. A population genetics study of the 12 X-STRs was conducted in a northwestern Italian population sample (n=160, 80 women and 80 men). A diallelic pattern at locus DXS6789 was observed in one man.

Available only for authorised users

Szibor R, Krawczak M, Hering S, Edelmann J, Kuhlisch E, Krause D (2003) Use of X-linked markers for forensic purposes. Int J Legal Med 117:67–74PubMed

Bini C, Ceccardi S, Ferri G et al (2005) Development of a heptaplex PCR system to analyse X-chromosome STR loci from five Italian population samples. A collaborative study. Forensic Sci Int 153:231–236PubMedCrossRef

Tabbada KA, De Ungria MC, Faustino LP, Athanasiadou D, Stradmann-Bellinghausen B, Schneider PM (2005) Development of a pentaplex X-chromosomal short tandem repeat typing system and population genetics studies. Forensic Sci Int 154:173–180PubMedCrossRef

Poetsch M, Petersmann H, Repenning A, Lignitz E (2005) Development of two pentaplex systems with X-chromosomal STR loci and their allele frequencies in a northeast German population. Forensic Sci Int 155:71–76PubMedCrossRef

Edelmann J, Hering S, Kuhlisch E, Szibor R (2002) Validation of the STR DXS7424 and the linkage situation on the X-chromosome. Forensic Sci Int 125:217–222PubMedCrossRef

Szibor R, Hering S, Kuhlisch E, Plate I, Demberger S, Krawczak M, Edelmann J (2005) Haplotyping of STR cluster DXS6801–DXS6809–DXS6789 on Xq21 provides a powerful tool for kinship testing. Int J Legal Med 119:363–369PubMedCrossRef

Kong A, Gudbjartsson DF, Sainz J et al (2002) A high-resolution recombination map of the human genome. Nat Genet 31:241–247PubMed

Szibor R, Hering S, Edelmann J (2005) A new Web site compiling forensic chromosome X research is now online. Int J Legal Med 120(4):252–254 DOI 10.1007/s00414-005-0029-y

Szibor R, Edelmann J, Hering S et al (2003) Cell line DNA typing in forensic genetics—the necessity of reliable standards. Forensic Sci Int 138:37–43PubMedCrossRef

10.

Edelmann J, Szibor R (2005) Validation of the X-linked STR DXS6801. Forensic Sci Int 148:219–220PubMedCrossRef

11.

Hering S, Brundirs N, Kuhlisch E et al (2004) DXS10011: studies on structure, allele distribution in three populations and genetic linkage to further q-telomeric chromosome X markers. Int J Legal Med 118:313–319CrossRef

12.

Bär W, Brinkmann B, Budowle B et al (1997) DNA recommendations. Further report of the DNA Commission of the ISFH regarding the use of short tandem repeat systems. International Society for Forensic Haemogenetics. Int J Legal Med 110:175–176PubMedCrossRef

13.

Edelmann J, Hering S, Michael M et al (2001) 16 X-chromosome STR loci frequency data from a German population. Forensic Sci Int 124:215–218CrossRef

14.

Edelmann J, Deichsel D, Plate I, Käser M, Szibor R (2003) Validation of the X-chromosomal STR DXS6809. Int J Legal Med 117:241–244PubMedCrossRef

15.

Edelmann J, Deichsel D, Hering S, Plate I, Szibor R (2002) Sequence variation and allele nomenclature for the X-linked STRs DXS9895, DXS8378, DXS7132, DXS6800, DXS7133, GATA172D05, DXS7423 and DXS8377. Forensic Sci Int 129:99–103PubMedCrossRef

16.

Schneider S, Roessli D, Excoffier L (2000) Arlequin version 2.000: A software for population genetics data analysis. Genetics and Biometry Laboratory, University of Geneva, Switzerland

17.

Nei M, Roychoudhury AK (1974) Sampling variances of heterozygosity and genetic distance. Genetics 76:379–390PubMed

18.

Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331PubMed

19.

Desmarais D, Zhong Y, Chakraborty R, Perreault C, Busque L (1998) Development of a highly polymorphic STR marker for identity testing purposes at the human androgen receptor gene (HUMARA). J Forensic Sci 43:1046–1049PubMed

20.

Krüger J, Fuhrmann W, Lichte KH, Steffens C (1968) Zur Verwendung der sauren Erythrocytenphosphatase bei der Vaterschaftsbegutachtung. Dtsch Z Gesamte Gerichtl Med 64:127–146PubMedCrossRef

21.

Kishida T, Tamaki Y (1997) Japanese population data on X-chromosomal STR locus AR. Nippon Hoigaku Zasshi 51:376–379PubMed

22.

Wiegand P, Berger B, Edelmann J, Parson W (2003) Population genetic comparisons of three X-chromosomal STRs. Int J Legal Med 117:62–65PubMed

23.

Lee HY, Park MJ, Jeong CK et al (2004) Genetic characteristics and population study of 4 X-chromosomal STRs in Koreans: evidence for a null allele at DXS9898. Int J Legal Med 118:355–360PubMedCrossRef

24.

Turrina S, De Leo D (2003) Population data of three X-chromosomal STRs: DXS7132, DXS7133 and GATA172D05 in north Italy. J Forensic Sci 48:1428–1429PubMed

25.

Toni C, Presciuttini S, Spinetti I, Domenici R (2003) Population data of four X-chromosome markers in Tuscany, and their use in a deficiency paternity case. Forensic Sci Int 137:215–216PubMed

26.

Iafrate AJ, Feuk L, Rivera MN et al (2004) Detection of large-scale variation in the human genome. Nat Genet 36:949–951PubMedCrossRef

27.

Shaw CJ, Lupski JR (2004) Implications of human genome architecture for rearrangement-based disorders: the genomic basis of disease. Hum Mol Genet 13(Spec No 1):R57–R64PubMedCrossRef

Title: Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample
Authors: C. Robino
A. Giolitti
S. Gino
C. Torre
Publication date: 01-09-2006
Publisher: Springer-Verlag
Published in: International Journal of Legal Medicine / Issue 5/2006
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-006-0115-9

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Development of two multiplex PCR systems for the analysis of 12 X-chromosomal STR loci in a northwestern Italian population sample

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 5/2006

Announcement

Japanese population data for eight X-STR loci using two new quadruplex systems

Margaret M. Stark (ed), Clinical forensic medicine—a physician's guide, 2nd edn Humana Press, Totowa, New Jersey, 2005 ISBN 1-588-29-368-8

An immunohistochemical study in a fatality due to ovarian hyperstimulation syndrome

Application of a quasi-median network analysis for the visualization of character conflicts to a population sample of mitochondrial DNA control region sequences from southern Germany (Ulm)

Gunshot-related transport of micro-organisms from the skin of the entrance region into the bullet path