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01-01-2007 | Technical Note

Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip

Authors: Sabine Lutz-Bonengel, Timo Sänger, Marielle Heinrich, Ulrike Schön, Ulrike Schmidt

Published in: International Journal of Legal Medicine | Issue 1/2007

Abstract

Low volume (LV) amplification (1 μL) of nuclear DNA (nucDNA) on a chemically structured chip is an appropriate and highly sensitive method to simultaneously amplify amelogenin and 15 forensically relevant short tandem repeats (STR). In this study, a combined method using on-chip LV amplification of mitochondrial DNA (mtDNA) and subsequent on-chip LV cycle sequencing was established to obtain a method, which is sensitive and robust enough to allow reliable analysis of DNA amounts representing the single cell level. All the necessary steps of the procedure—except for the purification of the sequencing products—were accomplished within the same final 2-μL reaction volume.

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Title: Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip
Authors: Sabine Lutz-Bonengel
Timo Sänger
Marielle Heinrich
Ulrike Schön
Ulrike Schmidt
Publication date: 01-01-2007
Publisher: Springer-Verlag
Published in: International Journal of Legal Medicine / Issue 1/2007
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-006-0125-7

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