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01-10-2003 | Original Article

Rapid screening of mtDNA coding region SNPs for the identification of west European Caucasian haplogroups

Authors: Anita Brandstätter, Thomas J. Parsons, Walther Parson

Published in: International Journal of Legal Medicine | Issue 5/2003

Abstract

This work presents a selection of 16 SNPs from the coding region of the human mitochondrial DNA. The selected markers are used for the assignment of individuals to one of the nine major European Caucasian mitochondrial haplogroups. The selected SNPs are targeted in two multiplex systems, via the application of the SNaPshot kit, a multiplex method based on the dideoxy single-base extension of unlabeled oligonucleotide primers. The method is conceived as a rapid screening technique prior to sequencing analysis, in order to eliminate multiple suspects from an inquiry or to discriminate between stains in a high volume casework example. Moreover, the ability to assign an unknown sample to an mtDNA type of known Caucasian origin could be of probative value in some investigations. A database of 277 Austrian Caucasians has been constructed, and the probability of a chance match between two unrelated individuals is calculated as 11.4%. This novel multiplex PCR amplification and typing system for mtDNA coding region SNPs promises to be a convenient and informative new DNA profiling system in the forensic field.

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Title: Rapid screening of mtDNA coding region SNPs for the identification of west European Caucasian haplogroups
Authors: Anita Brandstätter
Thomas J. Parsons
Walther Parson
Publication date: 01-10-2003
Publisher: Springer-Verlag
Published in: International Journal of Legal Medicine / Issue 5/2003
Print ISSN: 0937-9827
Electronic ISSN: 1437-1596
DOI: https://doi.org/10.1007/s00414-003-0395-2

At a glance: The STEP trials

Springer Medicine

Rapid screening of mtDNA coding region SNPs for the identification of west European Caucasian haplogroups

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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