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01-01-2010 | Original Paper

Progranulin expression is upregulated after spinal contusion in mice

Authors: Swati B. Naphade, Kristina A. Kigerl, Lyn B. Jakeman, Sandra K. Kostyk, Phillip G. Popovich, Jeff Kuret

Published in: Acta Neuropathologica | Issue 1/2010

Abstract

Progranulin (proepithelin) is a pleiotropic growth-factor associated with inflammation and wound repair in peripheral tissues. It also has been implicated in the response to acute traumatic brain injury as well as to chronic neurodegenerative diseases. To determine whether changes in progranulin expression also accompany acute spinal cord injury, C57BL/6 mice were subjected to mid-thoracic (T9 level) contusion spinal cord injury and analyzed by immunohistochemical and biochemical methods. Whereas spinal cord sections prepared from non-injured laminectomy control animals contained low basal levels of progranulin immunoreactivity in gray matter, sections from injured animals contained intense immunoreactivity throughout the injury epicenter that peaked 7–14 days post injury. Progranulin immunoreactivity colocalized with myeloid cell markers CD11b and CD68, indicating that expression increased primarily in activated microglia and macrophages. Immunoblot analysis confirmed that progranulin protein levels rose after injury. On the basis of quantitative polymerase chain reaction analysis, increased protein levels resulted from a tenfold rise in progranulin transcripts. These data demonstrate that progranulin is dramatically induced in myeloid cells after experimental spinal cord injury and is positioned appropriately both spatially and temporally to influence recovery after injury.

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Title: Progranulin expression is upregulated after spinal contusion in mice
Authors: Swati B. Naphade
Kristina A. Kigerl
Lyn B. Jakeman
Sandra K. Kostyk
Phillip G. Popovich
Jeff Kuret
Publication date: 01-01-2010
Publisher: Springer-Verlag
Published in: Acta Neuropathologica / Issue 1/2010
Print ISSN: 0001-6322
Electronic ISSN: 1432-0533
DOI: https://doi.org/10.1007/s00401-009-0616-y

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Progranulin expression is upregulated after spinal contusion in mice

Abstract

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