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European Journal of Nutrition
Published in: European Journal of Nutrition 2/2020

Open Access 01-03-2020 | Melanoma | Original Contribution

Allyl isothiocyanate regulates lysine acetylation and methylation marks in an experimental model of malignant melanoma

Authors: Melina Mitsiogianni, Theodora Mantso, Dimitrios T. Trafalis, H. P. Vasantha Rupasinghe, Vasilis Zoumpourlis, Rodrigo Franco, Sotiris Botaitis, Aglaia Pappa, Mihalis I. Panayiotidis

Published in: European Journal of Nutrition | Issue 2/2020

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Abstract

Objective(s)

Isothiocyanates (ITCs) are biologically active plant secondary metabolites capable of mediating various biological effects including modulation of the epigenome. Our aim was to characterize the effect of allyl isothiocyanate (AITC) on lysine acetylation and methylation marks as a potential epigenetic-induced anti-melanoma strategy.

Methods

Our malignant melanoma model consisted of (1) human (A375) and murine (B16-F10) malignant melanoma as well as of human; (2) brain (VMM1) and lymph node (Hs 294T) metastatic melanoma; (3) non-melanoma epidermoid carcinoma (A431) and (4) immortalized keratinocyte (HaCaT) cells subjected to AITC. Cell viability, histone deacetylases (HDACs) and acetyltransferases (HATs) activities were evaluated by the Alamar blue, Epigenase HDAC Activity/Inhibition and EpiQuik HAT Activity/Inhibition assay kits, respectively, while their expression levels together with those of lysine acetylation and methylation marks by western immunoblotting. Finally, apoptotic gene expression was assessed by an RT-PCR-based gene expression profiling methodology.

Results

AITC reduces cell viability, decreases HDACs and HATs activities and causes changes in protein expression levels of various HDACs, HATs, and histone methyl transferases (HMTs) all of which have a profound effect on specific lysine acetylation and methylation marks. Moreover, AITC regulates the expression of a number of genes participating in various apoptotic cascades thus indicating its involvement in apoptotic induction.

Conclusions

AITC exerts a potent epigenetic effect suggesting its potential involvement as a promising epigenetic-induced bioactive for the treatment of malignant melanoma.
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Metadata
Title
Allyl isothiocyanate regulates lysine acetylation and methylation marks in an experimental model of malignant melanoma
Authors
Melina Mitsiogianni
Theodora Mantso
Dimitrios T. Trafalis
H. P. Vasantha Rupasinghe
Vasilis Zoumpourlis
Rodrigo Franco
Sotiris Botaitis
Aglaia Pappa
Mihalis I. Panayiotidis
Publication date
01-03-2020
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Nutrition / Issue 2/2020
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-019-01925-6

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