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European Journal of Nutrition
Published in: European Journal of Nutrition 7/2019

01-10-2019 | Original Contribution

Effects of probiotics, prebiotics or synbiotics on jawbone in obese-insulin resistant rats

Authors: Sathima Eaimworawuthikul, Wannipa Tunapong, Titikorn Chunchai, Sakawdaurn Yasom, Keerati Wanchai, Panan Suntornsaratoon, Narattaphol Charoenphandhu, Parameth Thiennimitr, Nipon Chattipakorn, Siriporn C. Chattipakorn

Published in: European Journal of Nutrition | Issue 7/2019

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Abstract

Purpose

Chronic high-fat diet (HFD) consumption results in gut dysbiosis, systemic inflammation, obese-insulin resistance, and osteoporosis of the jawbones. The probiotics, prebiotics or synbiotics alleviated gut dysbiosis and the metabolic disturbance in HFD-induced obesity. However, the effects on jawbone properties have not been investigated. This study aimed to investigate the effects of probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on the jawbone properties along with metabolic parameters, gut and systemic inflammation in HFD-fed rats.

Methods

Forty-eight male Wistar rats were fed with either a HFD or normal diet for 12 weeks. Rats in each group were subdivided into four subgroups to be treated with either vehicle, probiotics, prebiotics, or synbiotics for the additional 12 weeks. Blood samples, gut, bone marrows, and jawbones were collected to determine metabolic parameters, inflammation, and bone properties.

Results

The HFD-fed rats developed obese-insulin resistance, as indicated by increased body weight, dyslipidemia and decreased insulin sensitivity. Serum lipopolysaccharide levels and interleukin-6 mRNA expression in the ileum and bone marrows were elevated. Altered bone metabolism and the impaired jawbone properties were evident as indicated by decreased bone mineral density with increased trabecular separation. Reduced ultimate load and stiffness were observed in HFD-fed rats. Treatments with probiotics, prebiotics or synbiotics in HFD-fed rats improved metabolic parameters and reduced inflammation. However, no alterations in jawbone properties were found in all treatments.

Conclusion

The osteoporosis of the jawbone occurred in obese-insulin resistance, and treatments with probiotics, prebiotics and synbiotics were not sufficient to improve the jawbone properties.
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Metadata
Title
Effects of probiotics, prebiotics or synbiotics on jawbone in obese-insulin resistant rats
Authors
Sathima Eaimworawuthikul
Wannipa Tunapong
Titikorn Chunchai
Sakawdaurn Yasom
Keerati Wanchai
Panan Suntornsaratoon
Narattaphol Charoenphandhu
Parameth Thiennimitr
Nipon Chattipakorn
Siriporn C. Chattipakorn
Publication date
01-10-2019
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Nutrition / Issue 7/2019
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-018-1829-4

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