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01-06-2018 | Original Contribution

miR-124a expression contributes to the monophasic pattern of insulin secretion in islets from pregnant rats submitted to a low-protein diet

Authors: Kariny Cassia de Siqueira, Faena Moura de Lima, Fernanda Souza Lima, Marina Satie Taki, Clarissa Felfili da Cunha, Sílvia Regina de Lima Reis, Rafael Ludemann Camargo, Thiago Martins Batista, Emerielle Cristine Vanzela, Tarlliza Romanna Nardelli, Everardo Magalhães Carneiro, Silvana Bordin, Letícia Martins Ignácio-Souza, Márcia Queiroz Latorraca

Published in: European Journal of Nutrition | Issue 4/2018

Abstract

Purpose

To evaluate the role of miR-124a in the regulation of genes involved in insulin exocytosis and its effects on the kinetics of insulin secretion in pancreatic islets from pregnant rats submitted to a low-protein diet.

Methods

Adult control non-pregnant (CNP) and control pregnant (CP) rats were fed a normal protein diet (17%), whereas low-protein non-pregnant (LPNP) and low-protein pregnant (LPP) rats were fed a low-protein diet (6%) from days 1 to 15 of pregnancy. Kinetics of the glucose-induced insulin release and measurement of [Ca²⁺]_i in pancreatic islets were assessed by standard protocols. The miR-124a expression and gene transcriptions from pancreatic islets were determined by real-time polymerase chain reaction.

Results

In islets from LPP rats, the first phase of insulin release was abrogated. The AUC [Ca²⁺]_i from the LPP group was lower compared with the other groups. miR-124a expression was reduced by a low-protein diet. SNAP-25 mRNA, protein expression, and Rab3A protein content were lower in the LPP rats than in CP rats. Syntaxin 1A and Kir6.2 mRNA levels were decreased in islets from low-protein rats compared with control rats, whereas their protein content was reduced in islets from pregnant rats.

Conclusions

Loss of biphasic insulin secretion in islets from LPP rats appears to have resulted from reduced [Ca²⁺]_i due, at least in part, to Kir6.2 underexpression and from the changes in exocytotic elements that are influenced either directly or indirectly by miR-124a.

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Title: miR-124a expression contributes to the monophasic pattern of insulin secretion in islets from pregnant rats submitted to a low-protein diet
Authors: Kariny Cassia de Siqueira
Faena Moura de Lima
Fernanda Souza Lima
Marina Satie Taki
Clarissa Felfili da Cunha
Sílvia Regina de Lima Reis
Rafael Ludemann Camargo
Thiago Martins Batista
Emerielle Cristine Vanzela
Tarlliza Romanna Nardelli
Everardo Magalhães Carneiro
Silvana Bordin
Letícia Martins Ignácio-Souza
Márcia Queiroz Latorraca
Publication date: 01-06-2018
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Nutrition / Issue 4/2018
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-017-1425-z

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miR-124a expression contributes to the monophasic pattern of insulin secretion in islets from pregnant rats submitted to a low-protein diet

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

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Conclusions

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