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European Journal of Nutrition
Published in: European Journal of Nutrition 1/2018

01-02-2018 | Original Contribution

Rice bran protein hydrolysates reduce arterial stiffening, vascular remodeling and oxidative stress in rats fed a high-carbohydrate and high-fat diet

Authors: Ketmanee Senaphan, Weerapon Sangartit, Poungrat Pakdeechote, Veerapol Kukongviriyapan, Patchareewan Pannangpetch, Supawan Thawornchinsombut, Stephen E. Greenwald, Upa Kukongviriyapan

Published in: European Journal of Nutrition | Issue 1/2018

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Abstract

Purpose

Rice bran protein hydrolysates (RBPH) contain highly nutritional proteins and antioxidant compounds which show benefits against metabolic syndrome (MetS). Increased arterial stiffness and the components of MetS have been shown to be associated with an increased risk of cardiovascular disease. This study aimed to investigate whether RBPH could alleviate the metabolic disorders, arterial stiffening, vascular remodeling, and oxidative stress in rats fed a high-carbohydrate and high-fat (HCHF) diet.

Methods

Male Sprague–Dawley rats were fed either a standard chow and tap water or a HCHF diet and 15 % fructose solution for 16 weeks. HCHF rats were treated orally with RBPH (250 or 500 mg/kg/day) for the final 6 weeks of the experimental period.

Results

Rats fed with HCHF diet had hyperglycemia, insulin resistance, dyslipidemia, hypertension, increased aortic pulse wave velocity, aortic wall hypertrophy and vascular remodeling with increased MMP-2 and MMP-9 expression. RBPH supplementation significantly alleviated these alterations (P < 0.05). Moreover, RBPH reduced the levels of angiotensin-converting enzyme (ACE) and tumor necrosis factor-alpha in plasma. Oxidative stress was also alleviated after RBPH treatment by decreasing plasma malondialdehyde, reducing superoxide production and suppressing p47phox NADPH oxidase expression in the vascular tissues of HCHF rats. RBPH increased plasma nitrate/nitrite level and up-regulated eNOS expression in the aortas of HCHF-diet-fed rats, indicating that RBPH increased NO production.

Conclusion

RBPH mitigate the deleterious effects of HCHF through potential mechanisms involving enhanced NO bioavailability, anti-ACE, anti-inflammatory and antioxidant properties. RBPH could be used as dietary supplements to minimize oxidative stress and vascular alterations triggered by MetS.
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Metadata
Title
Rice bran protein hydrolysates reduce arterial stiffening, vascular remodeling and oxidative stress in rats fed a high-carbohydrate and high-fat diet
Authors
Ketmanee Senaphan
Weerapon Sangartit
Poungrat Pakdeechote
Veerapol Kukongviriyapan
Patchareewan Pannangpetch
Supawan Thawornchinsombut
Stephen E. Greenwald
Upa Kukongviriyapan
Publication date
01-02-2018
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Nutrition / Issue 1/2018
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-016-1311-0

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